Rapid detection of avian leukosis virus subgroup J by cross-priming amplification

Abstract Avian leukosis virus subgroup J (ALV-J) causes oncogenic disease in chickens in China, resulting in great harm to poultry production, and remains widespread in China. Herein, we employed a cross-priming amplification (CPA) approach and a nucleic acid detection device to establish a visual r...

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Autores principales:	Yong Xiang, Lizhen Li, Peng Liu, Ling Yan, Zeng Jiang, Yun Yu, Yu Li, Xiaoyan Chen, Weisheng Cao
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/791e6b7e8f714403b561277d9ba55970
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spelling	oai:doaj.org-article:791e6b7e8f714403b561277d9ba559702021-12-02T14:47:31ZRapid detection of avian leukosis virus subgroup J by cross-priming amplification10.1038/s41598-021-90479-x2045-2322https://doaj.org/article/791e6b7e8f714403b561277d9ba559702021-05-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-90479-xhttps://doaj.org/toc/2045-2322Abstract Avian leukosis virus subgroup J (ALV-J) causes oncogenic disease in chickens in China, resulting in great harm to poultry production, and remains widespread in China. Herein, we employed a cross-priming amplification (CPA) approach and a nucleic acid detection device to establish a visual rapid detection method for ALV-J. The sensitivity of CPA, polymerase chain reaction (PCR) and real-time PCR (RT-PCR) was compared, and the three methods were used to detect ALV-J in the cell cultures which inoculated with clinical plasma. The result showed when the amplification reaction was carried out at 60 °C for just 60 min, the sensitivity of CPA was 10 times higher than conventional PCR, with high specificity, which was comparable with RT-PCR, based on detection of 123 cell cultures which inoculated with clinical plasma, the coincidence rate with real-time PCR was 97.3% (71/73). CPA detection of ALV-J does not require an expensive PCR instrument; a simple water bath or incubator is sufficient for complete DNA amplification, and the closed nucleic acid detection device avoids aerosol pollution, making judgment of results more intuitive and objective. The CPA assay would be a promising simple, rapid and sensitive method for identification of ALV-J.Yong XiangLizhen LiPeng LiuLing YanZeng JiangYun YuYu LiXiaoyan ChenWeisheng CaoNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-10 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Yong Xiang Lizhen Li Peng Liu Ling Yan Zeng Jiang Yun Yu Yu Li Xiaoyan Chen Weisheng Cao Rapid detection of avian leukosis virus subgroup J by cross-priming amplification
description	Abstract Avian leukosis virus subgroup J (ALV-J) causes oncogenic disease in chickens in China, resulting in great harm to poultry production, and remains widespread in China. Herein, we employed a cross-priming amplification (CPA) approach and a nucleic acid detection device to establish a visual rapid detection method for ALV-J. The sensitivity of CPA, polymerase chain reaction (PCR) and real-time PCR (RT-PCR) was compared, and the three methods were used to detect ALV-J in the cell cultures which inoculated with clinical plasma. The result showed when the amplification reaction was carried out at 60 °C for just 60 min, the sensitivity of CPA was 10 times higher than conventional PCR, with high specificity, which was comparable with RT-PCR, based on detection of 123 cell cultures which inoculated with clinical plasma, the coincidence rate with real-time PCR was 97.3% (71/73). CPA detection of ALV-J does not require an expensive PCR instrument; a simple water bath or incubator is sufficient for complete DNA amplification, and the closed nucleic acid detection device avoids aerosol pollution, making judgment of results more intuitive and objective. The CPA assay would be a promising simple, rapid and sensitive method for identification of ALV-J.
format	article
author	Yong Xiang Lizhen Li Peng Liu Ling Yan Zeng Jiang Yun Yu Yu Li Xiaoyan Chen Weisheng Cao
author_facet	Yong Xiang Lizhen Li Peng Liu Ling Yan Zeng Jiang Yun Yu Yu Li Xiaoyan Chen Weisheng Cao
author_sort	Yong Xiang
title	Rapid detection of avian leukosis virus subgroup J by cross-priming amplification
title_short	Rapid detection of avian leukosis virus subgroup J by cross-priming amplification
title_full	Rapid detection of avian leukosis virus subgroup J by cross-priming amplification
title_fullStr	Rapid detection of avian leukosis virus subgroup J by cross-priming amplification
title_full_unstemmed	Rapid detection of avian leukosis virus subgroup J by cross-priming amplification
title_sort	rapid detection of avian leukosis virus subgroup j by cross-priming amplification
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/791e6b7e8f714403b561277d9ba55970
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Rapid detection of avian leukosis virus subgroup J by cross-priming amplification

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