The In Vitro Immunomodulatory Effects Of Gold Nanoparticles Synthesized From Hypoxis hemerocallidea Aqueous Extract And Hypoxoside On Macrophage And Natural Killer Cells

Abdulrahman M Elbagory,1 Ahmed A Hussein,2 Mervin Meyer1 1DST/Mintek Nanotechnology Innovation Centre, Department of Biotechnology, University of the Western Cape, Bellville 7535, South Africa; 2Chemistry Department, Cape Peninsula University of Technology, Bellville 7535, South AfricaCorrespondence...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Elbagory AM, Hussein AA, Meyer M
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2019
Materias:	green nanotechnology anti-inflammatory innate immune cells cytokines solid phase sandwich elisa Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/792242baee1349ceac2c559c61464ebd
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:792242baee1349ceac2c559c61464ebd
record_format	dspace
spelling	oai:doaj.org-article:792242baee1349ceac2c559c61464ebd2021-12-02T05:45:16ZThe In Vitro Immunomodulatory Effects Of Gold Nanoparticles Synthesized From Hypoxis hemerocallidea Aqueous Extract And Hypoxoside On Macrophage And Natural Killer Cells1178-2013https://doaj.org/article/792242baee1349ceac2c559c61464ebd2019-11-01T00:00:00Zhttps://www.dovepress.com/the-in-vitro-immunomodulatory-effects-of-gold-nanoparticles-synthesize-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Abdulrahman M Elbagory,1 Ahmed A Hussein,2 Mervin Meyer1 1DST/Mintek Nanotechnology Innovation Centre, Department of Biotechnology, University of the Western Cape, Bellville 7535, South Africa; 2Chemistry Department, Cape Peninsula University of Technology, Bellville 7535, South AfricaCorrespondence: Mervin MeyerDepartment of Biotechnology, University of the Western Cape, Private Bag X17, Bellville 7535, South AfricaTel +27 21 959 2032Fax +27 21 959 3505Email memeyer@uwc.ac.zaBackground: Macrophages and Natural Killer (NK) cells are an integral part of the innate immune system. These cells produce pro-inflammatory cytokines in response to bacterial infections. However, prolonged inflammation can be a contributing factor in the etiology of several diseases such as rheumatoid arthritis, inflammatory bowel disease, multiple sclerosis, psoriasis and eczema. Reducing the secretion of pro-inflammatory cytokines is an effective treatment strategy for these conditions. Gold nanoparticles (AuNPs) have been shown to have immunosuppressive effects. Extracts of the Hypoxis hemerocallidea plant have also been shown to have immunomodulatory effects. It has been demonstrated previously that extracts of the H. hemerocallidea can be used to synthesize AuNPs.Purpose: This study aimed to investigate whether AuNPs synthesized using H. hemerocallidea extract and its major secondary metabolite, hypoxoside, have any immunomodulatory effects in macrophages and NK cells.Methodology: AuNPs derived from the H. hemerocallidea extract were synthesized as previously described. Using similar methodologies, this study shows for the first time the synthesis of AuNPs from hypoxoside. The AuNPs were characterized using several optical and spectroscopic techniques. The immunomodulatory effects of the aqueous extract of H. hemerocallidea, hypoxoside, as well as the AuNPs produced from the extract and hypoxoside, were investigated by measuring the cytokine levels in macrophages (IL-1β, IL-6 and TNF-α) and NK cells (IFN-γ) using solid phase sandwich ELISA technique.Results: The results show that spherical AuNPs (average size 26 ± 2 nm) were synthesized from hypoxoside. The results also show that the four treatments (H. hemerocallidea extract, hypoxoside and their respective AuNPs can lower the pro-inflammatory cytokine levels in the macrophages cells, while only AuNPs produced from hypoxoside can reduce cytokine responses in NK cells.Conclusion: This study shows that all four treatments investigated here could be further explored for the development of anti-inflammatory therapies.Keywords: green nanotechnology, gold nanoparticles, Hypoxis hemerocallidea, hypoxoside, anti-inflammatory, innate immune cells, cytokines, ELISAElbagory AMHussein AAMeyer MDove Medical Pressarticlegreen nanotechnologyanti-inflammatoryinnate immune cellscytokinessolid phase sandwich elisaMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 14, Pp 9007-9018 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	green nanotechnology anti-inflammatory innate immune cells cytokines solid phase sandwich elisa Medicine (General) R5-920
spellingShingle	green nanotechnology anti-inflammatory innate immune cells cytokines solid phase sandwich elisa Medicine (General) R5-920 Elbagory AM Hussein AA Meyer M The In Vitro Immunomodulatory Effects Of Gold Nanoparticles Synthesized From Hypoxis hemerocallidea Aqueous Extract And Hypoxoside On Macrophage And Natural Killer Cells
description	Abdulrahman M Elbagory,1 Ahmed A Hussein,2 Mervin Meyer1 1DST/Mintek Nanotechnology Innovation Centre, Department of Biotechnology, University of the Western Cape, Bellville 7535, South Africa; 2Chemistry Department, Cape Peninsula University of Technology, Bellville 7535, South AfricaCorrespondence: Mervin MeyerDepartment of Biotechnology, University of the Western Cape, Private Bag X17, Bellville 7535, South AfricaTel +27 21 959 2032Fax +27 21 959 3505Email memeyer@uwc.ac.zaBackground: Macrophages and Natural Killer (NK) cells are an integral part of the innate immune system. These cells produce pro-inflammatory cytokines in response to bacterial infections. However, prolonged inflammation can be a contributing factor in the etiology of several diseases such as rheumatoid arthritis, inflammatory bowel disease, multiple sclerosis, psoriasis and eczema. Reducing the secretion of pro-inflammatory cytokines is an effective treatment strategy for these conditions. Gold nanoparticles (AuNPs) have been shown to have immunosuppressive effects. Extracts of the Hypoxis hemerocallidea plant have also been shown to have immunomodulatory effects. It has been demonstrated previously that extracts of the H. hemerocallidea can be used to synthesize AuNPs.Purpose: This study aimed to investigate whether AuNPs synthesized using H. hemerocallidea extract and its major secondary metabolite, hypoxoside, have any immunomodulatory effects in macrophages and NK cells.Methodology: AuNPs derived from the H. hemerocallidea extract were synthesized as previously described. Using similar methodologies, this study shows for the first time the synthesis of AuNPs from hypoxoside. The AuNPs were characterized using several optical and spectroscopic techniques. The immunomodulatory effects of the aqueous extract of H. hemerocallidea, hypoxoside, as well as the AuNPs produced from the extract and hypoxoside, were investigated by measuring the cytokine levels in macrophages (IL-1β, IL-6 and TNF-α) and NK cells (IFN-γ) using solid phase sandwich ELISA technique.Results: The results show that spherical AuNPs (average size 26 ± 2 nm) were synthesized from hypoxoside. The results also show that the four treatments (H. hemerocallidea extract, hypoxoside and their respective AuNPs can lower the pro-inflammatory cytokine levels in the macrophages cells, while only AuNPs produced from hypoxoside can reduce cytokine responses in NK cells.Conclusion: This study shows that all four treatments investigated here could be further explored for the development of anti-inflammatory therapies.Keywords: green nanotechnology, gold nanoparticles, Hypoxis hemerocallidea, hypoxoside, anti-inflammatory, innate immune cells, cytokines, ELISA
format	article
author	Elbagory AM Hussein AA Meyer M
author_facet	Elbagory AM Hussein AA Meyer M
author_sort	Elbagory AM
title	The In Vitro Immunomodulatory Effects Of Gold Nanoparticles Synthesized From Hypoxis hemerocallidea Aqueous Extract And Hypoxoside On Macrophage And Natural Killer Cells
title_short	The In Vitro Immunomodulatory Effects Of Gold Nanoparticles Synthesized From Hypoxis hemerocallidea Aqueous Extract And Hypoxoside On Macrophage And Natural Killer Cells
title_full	The In Vitro Immunomodulatory Effects Of Gold Nanoparticles Synthesized From Hypoxis hemerocallidea Aqueous Extract And Hypoxoside On Macrophage And Natural Killer Cells
title_fullStr	The In Vitro Immunomodulatory Effects Of Gold Nanoparticles Synthesized From Hypoxis hemerocallidea Aqueous Extract And Hypoxoside On Macrophage And Natural Killer Cells
title_full_unstemmed	The In Vitro Immunomodulatory Effects Of Gold Nanoparticles Synthesized From Hypoxis hemerocallidea Aqueous Extract And Hypoxoside On Macrophage And Natural Killer Cells
title_sort	in vitro immunomodulatory effects of gold nanoparticles synthesized from hypoxis hemerocallidea aqueous extract and hypoxoside on macrophage and natural killer cells
publisher	Dove Medical Press
publishDate	2019
url	https://doaj.org/article/792242baee1349ceac2c559c61464ebd
work_keys_str_mv	AT elbagoryam theinvitroimmunomodulatoryeffectsofgoldnanoparticlessynthesizedfromhypoxishemerocallideaaqueousextractandhypoxosideonmacrophageandnaturalkillercells AT husseinaa theinvitroimmunomodulatoryeffectsofgoldnanoparticlessynthesizedfromhypoxishemerocallideaaqueousextractandhypoxosideonmacrophageandnaturalkillercells AT meyerm theinvitroimmunomodulatoryeffectsofgoldnanoparticlessynthesizedfromhypoxishemerocallideaaqueousextractandhypoxosideonmacrophageandnaturalkillercells AT elbagoryam invitroimmunomodulatoryeffectsofgoldnanoparticlessynthesizedfromhypoxishemerocallideaaqueousextractandhypoxosideonmacrophageandnaturalkillercells AT husseinaa invitroimmunomodulatoryeffectsofgoldnanoparticlessynthesizedfromhypoxishemerocallideaaqueousextractandhypoxosideonmacrophageandnaturalkillercells AT meyerm invitroimmunomodulatoryeffectsofgoldnanoparticlessynthesizedfromhypoxishemerocallideaaqueousextractandhypoxosideonmacrophageandnaturalkillercells
_version_	1718400238257963008

The In Vitro Immunomodulatory Effects Of Gold Nanoparticles Synthesized From Hypoxis hemerocallidea Aqueous Extract And Hypoxoside On Macrophage And Natural Killer Cells

Ejemplares similares