Effect of Agrobacterium Infection Time, Co-Cultivation and Cell Density on in vitro Response in Hypocotyl of Eggplant (Solanum melongena L.)

The present study purports to assess the effect of Agrobacterium infection time, co-cultivation and cell density on in vitro response in hypocotyl explants of eggplant (brinjal) cv. Manjarigota. Agrobacterium(OD600 0.3-0.5) infection for 10-15 minutes (24.44±2.34%) was found to be optimum, while, hi...

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Autores principales:	D P Prakash, Y L Ramachandra, Vageeshbabu S Hanur
Formato:	article
Lenguaje:	EN
Publicado:	Society for Promotion of Horticulture - Indian Institute of Horticultural Research 2016
Materias:	eggplant manjarigota regeneration agrobacterium pcr Plant culture SB1-1110
Acceso en línea:	https://doaj.org/article/79299575034b46109fc2dba32c0ff209
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spelling	oai:doaj.org-article:79299575034b46109fc2dba32c0ff2092021-12-02T11:12:39ZEffect of Agrobacterium Infection Time, Co-Cultivation and Cell Density on in vitro Response in Hypocotyl of Eggplant (Solanum melongena L.)0973-354X2582-4899https://doaj.org/article/79299575034b46109fc2dba32c0ff2092016-06-01T00:00:00Zhttps://jhs.iihr.res.in/index.php/jhs/article/view/97https://doaj.org/toc/0973-354Xhttps://doaj.org/toc/2582-4899The present study purports to assess the effect of Agrobacterium infection time, co-cultivation and cell density on in vitro response in hypocotyl explants of eggplant (brinjal) cv. Manjarigota. Agrobacterium(OD600 0.3-0.5) infection for 10-15 minutes (24.44±2.34%) was found to be optimum, while, higher or lower infection-time resulted in reduced callus initiation, shoot regeneration and explant survival. Explants with no (only Agrobacterium infection) or short (1 day) co-cultivation, showed reduced callus-initiation response and turned yellow, with no regeneration. Callus-initiation response increased from Day 1 (96.66±03.33%), and reached a maximum on Day 2 and Day 3 (100±00.00%). It decreased on further increase in co-cultivation time. Explants co-cultivated for three days showed highest regeneration response (30.00±02.96%) which thereafter reduced with further increase in co-cultivation time. Explants infected with Agrobacterium culture at 0.05 OD600 showed hardly any regeneration, and turned yellow and necrotic on the selection medium. Highest regeneration response (28.33±02.33%) was obtained in explants infected with 0.1 OD600 culture, and this gradually reduced as celldensity increased (upto 1.0 OD600), becoming zero in explants treated with cultures at 1.5 OD600 or above. Agrobacterium overgrowth was noticed on explants infected with cultures of 0.5 OD600 and above. Exposure of hypocotyl explants to higher cell-density, longer infection-time and prolonged co-cultivation regime resulted in severe necrosis of explants; time taken for development of Agrobacterium overgrowth was less with increase in the level of these factors. Regenerated shoots were healthy, green, elongated and showed root induction on culture medium containing Kanamycin.D P PrakashY L RamachandraVageeshbabu S HanurSociety for Promotion of Horticulture - Indian Institute of Horticultural ResearcharticleeggplantmanjarigotaregenerationagrobacteriumpcrPlant cultureSB1-1110ENJournal of Horticultural Sciences, Vol 11, Iss 1, Pp 21-26 (2016)
institution	DOAJ
collection	DOAJ
language	EN
topic	eggplant manjarigota regeneration agrobacterium pcr Plant culture SB1-1110
spellingShingle	eggplant manjarigota regeneration agrobacterium pcr Plant culture SB1-1110 D P Prakash Y L Ramachandra Vageeshbabu S Hanur Effect of Agrobacterium Infection Time, Co-Cultivation and Cell Density on in vitro Response in Hypocotyl of Eggplant (Solanum melongena L.)
description	The present study purports to assess the effect of Agrobacterium infection time, co-cultivation and cell density on in vitro response in hypocotyl explants of eggplant (brinjal) cv. Manjarigota. Agrobacterium(OD600 0.3-0.5) infection for 10-15 minutes (24.44±2.34%) was found to be optimum, while, higher or lower infection-time resulted in reduced callus initiation, shoot regeneration and explant survival. Explants with no (only Agrobacterium infection) or short (1 day) co-cultivation, showed reduced callus-initiation response and turned yellow, with no regeneration. Callus-initiation response increased from Day 1 (96.66±03.33%), and reached a maximum on Day 2 and Day 3 (100±00.00%). It decreased on further increase in co-cultivation time. Explants co-cultivated for three days showed highest regeneration response (30.00±02.96%) which thereafter reduced with further increase in co-cultivation time. Explants infected with Agrobacterium culture at 0.05 OD600 showed hardly any regeneration, and turned yellow and necrotic on the selection medium. Highest regeneration response (28.33±02.33%) was obtained in explants infected with 0.1 OD600 culture, and this gradually reduced as celldensity increased (upto 1.0 OD600), becoming zero in explants treated with cultures at 1.5 OD600 or above. Agrobacterium overgrowth was noticed on explants infected with cultures of 0.5 OD600 and above. Exposure of hypocotyl explants to higher cell-density, longer infection-time and prolonged co-cultivation regime resulted in severe necrosis of explants; time taken for development of Agrobacterium overgrowth was less with increase in the level of these factors. Regenerated shoots were healthy, green, elongated and showed root induction on culture medium containing Kanamycin.
format	article
author	D P Prakash Y L Ramachandra Vageeshbabu S Hanur
author_facet	D P Prakash Y L Ramachandra Vageeshbabu S Hanur
author_sort	D P Prakash
title	Effect of Agrobacterium Infection Time, Co-Cultivation and Cell Density on in vitro Response in Hypocotyl of Eggplant (Solanum melongena L.)
title_short	Effect of Agrobacterium Infection Time, Co-Cultivation and Cell Density on in vitro Response in Hypocotyl of Eggplant (Solanum melongena L.)
title_full	Effect of Agrobacterium Infection Time, Co-Cultivation and Cell Density on in vitro Response in Hypocotyl of Eggplant (Solanum melongena L.)
title_fullStr	Effect of Agrobacterium Infection Time, Co-Cultivation and Cell Density on in vitro Response in Hypocotyl of Eggplant (Solanum melongena L.)
title_full_unstemmed	Effect of Agrobacterium Infection Time, Co-Cultivation and Cell Density on in vitro Response in Hypocotyl of Eggplant (Solanum melongena L.)
title_sort	effect of agrobacterium infection time, co-cultivation and cell density on in vitro response in hypocotyl of eggplant (solanum melongena l.)
publisher	Society for Promotion of Horticulture - Indian Institute of Horticultural Research
publishDate	2016
url	https://doaj.org/article/79299575034b46109fc2dba32c0ff209
work_keys_str_mv	AT dpprakash effectofagrobacteriuminfectiontimecocultivationandcelldensityoninvitroresponseinhypocotylofeggplantsolanummelongenal AT ylramachandra effectofagrobacteriuminfectiontimecocultivationandcelldensityoninvitroresponseinhypocotylofeggplantsolanummelongenal AT vageeshbabushanur effectofagrobacteriuminfectiontimecocultivationandcelldensityoninvitroresponseinhypocotylofeggplantsolanummelongenal
_version_	1718396114414075904

Effect of Agrobacterium Infection Time, Co-Cultivation and Cell Density on in vitro Response in Hypocotyl of Eggplant (Solanum melongena L.)

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