New insights into the genomic organization and splicing of the doublesex gene, a terminal regulator of sexual differentiation in the silkworm Bombyx mori.

Sex-determination mechanisms differ among organisms. The primary mechanism is diverse, whereas the terminal regulator is relatively-conserved. We analyzed the transcripts of the Bombyx mori doublesex gene (Bmdsx), and reported novel results concerning the genomic organization and expression of Bmdsx...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Jianping Duan, Hanfu Xu, Huizhen Guo, David A O'Brochta, Feng Wang, Sanyuan Ma, Liying Zhang, Xingfu Zha, Ping Zhao, Qingyou Xia
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/792a5c99cac6456888ffc05bec4156ab
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:792a5c99cac6456888ffc05bec4156ab
record_format	dspace
spelling	oai:doaj.org-article:792a5c99cac6456888ffc05bec4156ab2021-11-18T08:47:53ZNew insights into the genomic organization and splicing of the doublesex gene, a terminal regulator of sexual differentiation in the silkworm Bombyx mori.1932-620310.1371/journal.pone.0079703https://doaj.org/article/792a5c99cac6456888ffc05bec4156ab2013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24244545/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203Sex-determination mechanisms differ among organisms. The primary mechanism is diverse, whereas the terminal regulator is relatively-conserved. We analyzed the transcripts of the Bombyx mori doublesex gene (Bmdsx), and reported novel results concerning the genomic organization and expression of Bmdsx. Bmdsx consists of nine exons and eight introns, of which two exons are novel and have not been reported previously. Bmdsx transcripts are spliced to generate seventeen alternatively-spliced forms and eleven putative trans-spliced variants. Thirteen of the alternatively-spliced forms and five of the putative trans-spliced forms are reported here for the first time. Sequence analysis predicts that ten female-specific, six male-specific splice forms and one splice form found in males and females will result in four female-specific, two male-specific Dsx proteins and one Dsx protein common to males and females. The Dsx proteins are expected to be functional and regulate downstream target genes. Some of the predicted Dsx proteins are described here for the first time. Therefore the expression of the dsx gene in B. mori results in a variety of cis- and trans-spliced transcripts and multiple Dsx proteins. These findings show that in B. mori there is a complicated pattern of dsx splicing, and that the regulation of splicing and sex-specific functions of lepidopteran dsx have evolved complexity.Jianping DuanHanfu XuHuizhen GuoDavid A O'BrochtaFeng WangSanyuan MaLiying ZhangXingfu ZhaPing ZhaoQingyou XiaPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 11, p e79703 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Jianping Duan Hanfu Xu Huizhen Guo David A O'Brochta Feng Wang Sanyuan Ma Liying Zhang Xingfu Zha Ping Zhao Qingyou Xia New insights into the genomic organization and splicing of the doublesex gene, a terminal regulator of sexual differentiation in the silkworm Bombyx mori.
description	Sex-determination mechanisms differ among organisms. The primary mechanism is diverse, whereas the terminal regulator is relatively-conserved. We analyzed the transcripts of the Bombyx mori doublesex gene (Bmdsx), and reported novel results concerning the genomic organization and expression of Bmdsx. Bmdsx consists of nine exons and eight introns, of which two exons are novel and have not been reported previously. Bmdsx transcripts are spliced to generate seventeen alternatively-spliced forms and eleven putative trans-spliced variants. Thirteen of the alternatively-spliced forms and five of the putative trans-spliced forms are reported here for the first time. Sequence analysis predicts that ten female-specific, six male-specific splice forms and one splice form found in males and females will result in four female-specific, two male-specific Dsx proteins and one Dsx protein common to males and females. The Dsx proteins are expected to be functional and regulate downstream target genes. Some of the predicted Dsx proteins are described here for the first time. Therefore the expression of the dsx gene in B. mori results in a variety of cis- and trans-spliced transcripts and multiple Dsx proteins. These findings show that in B. mori there is a complicated pattern of dsx splicing, and that the regulation of splicing and sex-specific functions of lepidopteran dsx have evolved complexity.
format	article
author	Jianping Duan Hanfu Xu Huizhen Guo David A O'Brochta Feng Wang Sanyuan Ma Liying Zhang Xingfu Zha Ping Zhao Qingyou Xia
author_facet	Jianping Duan Hanfu Xu Huizhen Guo David A O'Brochta Feng Wang Sanyuan Ma Liying Zhang Xingfu Zha Ping Zhao Qingyou Xia
author_sort	Jianping Duan
title	New insights into the genomic organization and splicing of the doublesex gene, a terminal regulator of sexual differentiation in the silkworm Bombyx mori.
title_short	New insights into the genomic organization and splicing of the doublesex gene, a terminal regulator of sexual differentiation in the silkworm Bombyx mori.
title_full	New insights into the genomic organization and splicing of the doublesex gene, a terminal regulator of sexual differentiation in the silkworm Bombyx mori.
title_fullStr	New insights into the genomic organization and splicing of the doublesex gene, a terminal regulator of sexual differentiation in the silkworm Bombyx mori.
title_full_unstemmed	New insights into the genomic organization and splicing of the doublesex gene, a terminal regulator of sexual differentiation in the silkworm Bombyx mori.
title_sort	new insights into the genomic organization and splicing of the doublesex gene, a terminal regulator of sexual differentiation in the silkworm bombyx mori.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/792a5c99cac6456888ffc05bec4156ab
work_keys_str_mv	AT jianpingduan newinsightsintothegenomicorganizationandsplicingofthedoublesexgeneaterminalregulatorofsexualdifferentiationinthesilkwormbombyxmori AT hanfuxu newinsightsintothegenomicorganizationandsplicingofthedoublesexgeneaterminalregulatorofsexualdifferentiationinthesilkwormbombyxmori AT huizhenguo newinsightsintothegenomicorganizationandsplicingofthedoublesexgeneaterminalregulatorofsexualdifferentiationinthesilkwormbombyxmori AT davidaobrochta newinsightsintothegenomicorganizationandsplicingofthedoublesexgeneaterminalregulatorofsexualdifferentiationinthesilkwormbombyxmori AT fengwang newinsightsintothegenomicorganizationandsplicingofthedoublesexgeneaterminalregulatorofsexualdifferentiationinthesilkwormbombyxmori AT sanyuanma newinsightsintothegenomicorganizationandsplicingofthedoublesexgeneaterminalregulatorofsexualdifferentiationinthesilkwormbombyxmori AT liyingzhang newinsightsintothegenomicorganizationandsplicingofthedoublesexgeneaterminalregulatorofsexualdifferentiationinthesilkwormbombyxmori AT xingfuzha newinsightsintothegenomicorganizationandsplicingofthedoublesexgeneaterminalregulatorofsexualdifferentiationinthesilkwormbombyxmori AT pingzhao newinsightsintothegenomicorganizationandsplicingofthedoublesexgeneaterminalregulatorofsexualdifferentiationinthesilkwormbombyxmori AT qingyouxia newinsightsintothegenomicorganizationandsplicingofthedoublesexgeneaterminalregulatorofsexualdifferentiationinthesilkwormbombyxmori
_version_	1718421270564962304

New insights into the genomic organization and splicing of the doublesex gene, a terminal regulator of sexual differentiation in the silkworm Bombyx mori.

Ejemplares similares