Synthesis and Biodistribution of <sup>99m</sup>Tc-Labeled PLGA Nanoparticles by Microfluidic Technique

The aim of present study was to develop radiolabeled NPs to overcome the limitations of fluorescence with theranostic potential. Synthesis of PLGA-NPs loaded with technetium-99m was based on a Dean-Vortex-Bifurcation Mixer (DVBM) using an innovative microfluidic technique with high batch-to-batch re...

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Autores principales:	Michela Varani, Giuseppe Campagna, Valeria Bentivoglio, Matteo Serafinelli, Maria Luisa Martini, Filippo Galli, Alberto Signore
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	radiolabeled nanoparticles poly (lactic-co-glycolic acid) (PLGA) nuclear medicine microfluidics Pharmacy and materia medica RS1-441
Acceso en línea:	https://doaj.org/article/792f0433986c4bbeb8b8858b6f2d7efd
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spelling	oai:doaj.org-article:792f0433986c4bbeb8b8858b6f2d7efd2021-11-25T18:40:33ZSynthesis and Biodistribution of <sup>99m</sup>Tc-Labeled PLGA Nanoparticles by Microfluidic Technique10.3390/pharmaceutics131117691999-4923https://doaj.org/article/792f0433986c4bbeb8b8858b6f2d7efd2021-10-01T00:00:00Zhttps://www.mdpi.com/1999-4923/13/11/1769https://doaj.org/toc/1999-4923The aim of present study was to develop radiolabeled NPs to overcome the limitations of fluorescence with theranostic potential. Synthesis of PLGA-NPs loaded with technetium-99m was based on a Dean-Vortex-Bifurcation Mixer (DVBM) using an innovative microfluidic technique with high batch-to-batch reproducibility and tailored-made size of NPs. Eighteen different formulations were tested and characterized for particle size, zeta potential, polydispersity index, labeling efficiency, and in vitro stability. Overall, physical characterization by dynamic light scattering (DLS) showed an increase in particle size after radiolabeling probably due to the incorporation of the isotope into the PLGA-NPs shell. NPs of 60 nm (obtained by 5:1 PVA:PLGA ratio and 15 mL/min TFR with <sup>99m</sup>Tc included in PVA) had high labeling efficiency (94.20 ± 5.83%) and >80% stability after 24 h and showed optimal biodistribution in BALB/c mice. In conclusion, we confirmed the possibility of radiolabeling NPs with <sup>99m</sup>Tc using the microfluidics and provide best formulation for tumor targeting studies.Michela VaraniGiuseppe CampagnaValeria BentivoglioMatteo SerafinelliMaria Luisa MartiniFilippo GalliAlberto SignoreMDPI AGarticleradiolabeled nanoparticlespoly (lactic-co-glycolic acid) (PLGA)nuclear medicinemicrofluidicsPharmacy and materia medicaRS1-441ENPharmaceutics, Vol 13, Iss 1769, p 1769 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	radiolabeled nanoparticles poly (lactic-co-glycolic acid) (PLGA) nuclear medicine microfluidics Pharmacy and materia medica RS1-441
spellingShingle	radiolabeled nanoparticles poly (lactic-co-glycolic acid) (PLGA) nuclear medicine microfluidics Pharmacy and materia medica RS1-441 Michela Varani Giuseppe Campagna Valeria Bentivoglio Matteo Serafinelli Maria Luisa Martini Filippo Galli Alberto Signore Synthesis and Biodistribution of <sup>99m</sup>Tc-Labeled PLGA Nanoparticles by Microfluidic Technique
description	The aim of present study was to develop radiolabeled NPs to overcome the limitations of fluorescence with theranostic potential. Synthesis of PLGA-NPs loaded with technetium-99m was based on a Dean-Vortex-Bifurcation Mixer (DVBM) using an innovative microfluidic technique with high batch-to-batch reproducibility and tailored-made size of NPs. Eighteen different formulations were tested and characterized for particle size, zeta potential, polydispersity index, labeling efficiency, and in vitro stability. Overall, physical characterization by dynamic light scattering (DLS) showed an increase in particle size after radiolabeling probably due to the incorporation of the isotope into the PLGA-NPs shell. NPs of 60 nm (obtained by 5:1 PVA:PLGA ratio and 15 mL/min TFR with <sup>99m</sup>Tc included in PVA) had high labeling efficiency (94.20 ± 5.83%) and >80% stability after 24 h and showed optimal biodistribution in BALB/c mice. In conclusion, we confirmed the possibility of radiolabeling NPs with <sup>99m</sup>Tc using the microfluidics and provide best formulation for tumor targeting studies.
format	article
author	Michela Varani Giuseppe Campagna Valeria Bentivoglio Matteo Serafinelli Maria Luisa Martini Filippo Galli Alberto Signore
author_facet	Michela Varani Giuseppe Campagna Valeria Bentivoglio Matteo Serafinelli Maria Luisa Martini Filippo Galli Alberto Signore
author_sort	Michela Varani
title	Synthesis and Biodistribution of <sup>99m</sup>Tc-Labeled PLGA Nanoparticles by Microfluidic Technique
title_short	Synthesis and Biodistribution of <sup>99m</sup>Tc-Labeled PLGA Nanoparticles by Microfluidic Technique
title_full	Synthesis and Biodistribution of <sup>99m</sup>Tc-Labeled PLGA Nanoparticles by Microfluidic Technique
title_fullStr	Synthesis and Biodistribution of <sup>99m</sup>Tc-Labeled PLGA Nanoparticles by Microfluidic Technique
title_full_unstemmed	Synthesis and Biodistribution of <sup>99m</sup>Tc-Labeled PLGA Nanoparticles by Microfluidic Technique
title_sort	synthesis and biodistribution of <sup>99m</sup>tc-labeled plga nanoparticles by microfluidic technique
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/792f0433986c4bbeb8b8858b6f2d7efd
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Synthesis and Biodistribution of <sup>99m</sup>Tc-Labeled PLGA Nanoparticles by Microfluidic Technique

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