New properties of Drosophila scs and scs' insulators.

Insulators are defined as a class of regulatory elements that delimit independent transcriptional domains within eukaryotic genomes. The first insulators to be identified were scs and scs', which flank the domain including two heat shock 70 genes. Zw5 and BEAF bind to scs and scs', respect...

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Autores principales:	Olga Kyrchanova, Dmitry Leman, Alexander Parshikov, Anna Fedotova, Vasily Studitsky, Oksana Maksimenko, Pavel Georgiev
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/79329b799cf9494aa2c2ffaa4d7b0181
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spelling	oai:doaj.org-article:79329b799cf9494aa2c2ffaa4d7b01812021-11-18T07:47:56ZNew properties of Drosophila scs and scs' insulators.1932-620310.1371/journal.pone.0062690https://doaj.org/article/79329b799cf9494aa2c2ffaa4d7b01812013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23638134/?tool=EBIhttps://doaj.org/toc/1932-6203Insulators are defined as a class of regulatory elements that delimit independent transcriptional domains within eukaryotic genomes. The first insulators to be identified were scs and scs', which flank the domain including two heat shock 70 genes. Zw5 and BEAF bind to scs and scs', respectively, and are responsible for the interaction between these insulators. Using the regulatory regions of yellow and white reporter genes, we have found that the interaction between scs and scs' improves the enhancer-blocking activity of the weak scs' insulator. The sequences of scs and scs' insulators include the promoters of genes that are strongly active in S2 cells but not in the eyes, in which the enhancer-blocking activity of these insulators has been extensively examined. Only the promoter of the Cad87A gene located at the end of the scs insulator drives white expression in the eyes, and the white enhancer can slightly stimulate this promoter. The scs insulator contains polyadenylation signals that may be important for preventing transcription through the insulator. As shown previously, scs and scs' can insulate transcription of the white transgene from the enhancing effects of the surrounding genome, a phenomenon known as the chromosomal position effect (CPE). After analyzing many independent transgenic lines, we have concluded that transgenes carrying the scs insulator are rarely inserted into genomic regions that stimulate the white reporter expression in the eyes.Olga KyrchanovaDmitry LemanAlexander ParshikovAnna FedotovaVasily StuditskyOksana MaksimenkoPavel GeorgievPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 4, p e62690 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Olga Kyrchanova Dmitry Leman Alexander Parshikov Anna Fedotova Vasily Studitsky Oksana Maksimenko Pavel Georgiev New properties of Drosophila scs and scs' insulators.
description	Insulators are defined as a class of regulatory elements that delimit independent transcriptional domains within eukaryotic genomes. The first insulators to be identified were scs and scs', which flank the domain including two heat shock 70 genes. Zw5 and BEAF bind to scs and scs', respectively, and are responsible for the interaction between these insulators. Using the regulatory regions of yellow and white reporter genes, we have found that the interaction between scs and scs' improves the enhancer-blocking activity of the weak scs' insulator. The sequences of scs and scs' insulators include the promoters of genes that are strongly active in S2 cells but not in the eyes, in which the enhancer-blocking activity of these insulators has been extensively examined. Only the promoter of the Cad87A gene located at the end of the scs insulator drives white expression in the eyes, and the white enhancer can slightly stimulate this promoter. The scs insulator contains polyadenylation signals that may be important for preventing transcription through the insulator. As shown previously, scs and scs' can insulate transcription of the white transgene from the enhancing effects of the surrounding genome, a phenomenon known as the chromosomal position effect (CPE). After analyzing many independent transgenic lines, we have concluded that transgenes carrying the scs insulator are rarely inserted into genomic regions that stimulate the white reporter expression in the eyes.
format	article
author	Olga Kyrchanova Dmitry Leman Alexander Parshikov Anna Fedotova Vasily Studitsky Oksana Maksimenko Pavel Georgiev
author_facet	Olga Kyrchanova Dmitry Leman Alexander Parshikov Anna Fedotova Vasily Studitsky Oksana Maksimenko Pavel Georgiev
author_sort	Olga Kyrchanova
title	New properties of Drosophila scs and scs' insulators.
title_short	New properties of Drosophila scs and scs' insulators.
title_full	New properties of Drosophila scs and scs' insulators.
title_fullStr	New properties of Drosophila scs and scs' insulators.
title_full_unstemmed	New properties of Drosophila scs and scs' insulators.
title_sort	new properties of drosophila scs and scs' insulators.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/79329b799cf9494aa2c2ffaa4d7b0181
work_keys_str_mv	AT olgakyrchanova newpropertiesofdrosophilascsandscsinsulators AT dmitryleman newpropertiesofdrosophilascsandscsinsulators AT alexanderparshikov newpropertiesofdrosophilascsandscsinsulators AT annafedotova newpropertiesofdrosophilascsandscsinsulators AT vasilystuditsky newpropertiesofdrosophilascsandscsinsulators AT oksanamaksimenko newpropertiesofdrosophilascsandscsinsulators AT pavelgeorgiev newpropertiesofdrosophilascsandscsinsulators
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New properties of Drosophila scs and scs' insulators.

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