Global analysis of glycoproteins identifies markers of endotoxin tolerant monocytes and GPR84 as a modulator of TNFα expression

Global analysis of glycoproteins identifies markers of endotoxin tolerant monocytes and GPR84 as a modulator of TNFα expression

Abstract Exposure of human monocytes to lipopolysaccharide (LPS) induces a temporary insensitivity to subsequent LPS challenges, a cellular state called endotoxin tolerance. In this study, we investigated the LPS-induced global glycoprotein expression changes of tolerant human monocytes and THP-1 ce...

Descripción completa

Guardado en:
Detalles Bibliográficos
Autores principales: Mario M. Müller, Roland Lehmann, Tilman E. Klassert, Stella Reifenstein, Theresia Conrad, Christoph Moore, Anna Kuhn, Andrea Behnert, Reinhard Guthke, Dominik Driesch, Hortense Slevogt
Formato: article
Lenguaje:EN
Publicado: Nature Portfolio 2017
Materias:
Medicine
R
Science
Q
Acceso en línea:https://doaj.org/article/7933dd5b970a4a05a24a95607778d7de
Etiquetas: Agregar Etiqueta
Sin Etiquetas, Sea el primero en etiquetar este registro!
id oai:doaj.org-article:7933dd5b970a4a05a24a95607778d7de
record_format dspace
spelling oai:doaj.org-article:7933dd5b970a4a05a24a95607778d7de2021-12-02T11:52:25ZGlobal analysis of glycoproteins identifies markers of endotoxin tolerant monocytes and GPR84 as a modulator of TNFα expression10.1038/s41598-017-00828-y2045-2322https://doaj.org/article/7933dd5b970a4a05a24a95607778d7de2017-04-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-00828-yhttps://doaj.org/toc/2045-2322Abstract Exposure of human monocytes to lipopolysaccharide (LPS) induces a temporary insensitivity to subsequent LPS challenges, a cellular state called endotoxin tolerance. In this study, we investigated the LPS-induced global glycoprotein expression changes of tolerant human monocytes and THP-1 cells to identify markers and glycoprotein targets capable to modulate the immunosuppressive state. Using hydrazide chemistry and LC-MS/MS analysis, we analyzed glycoprotein expression changes during a 48 h LPS time course. The cellular snapshots at different time points identified 1491 glycoproteins expressed by monocytes and THP-1 cells. Label-free quantitative analysis revealed transient or long-lasting LPS-induced expression changes of secreted or membrane-anchored glycoproteins derived from intracellular membrane coated organelles or from the plasma membrane. Monocytes and THP-1 cells demonstrated marked differences in glycoproteins differentially expressed in the tolerant state. Among the shared differentially expressed glycoproteins G protein-coupled receptor 84 (GPR84) was identified as being capable of modulating pro-inflammatory TNFα mRNA expression in the tolerant cell state when activated with its ligand Decanoic acid.Mario M. MüllerRoland LehmannTilman E. KlassertStella ReifensteinTheresia ConradChristoph MooreAnna KuhnAndrea BehnertReinhard GuthkeDominik DrieschHortense SlevogtNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-13 (2017)
institution DOAJ
collection DOAJ
language EN
topic Medicine
R
Science
Q
spellingShingle Medicine
R
Science
Q
Mario M. Müller
Roland Lehmann
Tilman E. Klassert
Stella Reifenstein
Theresia Conrad
Christoph Moore
Anna Kuhn
Andrea Behnert
Reinhard Guthke
Dominik Driesch
Hortense Slevogt
Global analysis of glycoproteins identifies markers of endotoxin tolerant monocytes and GPR84 as a modulator of TNFα expression
description Abstract Exposure of human monocytes to lipopolysaccharide (LPS) induces a temporary insensitivity to subsequent LPS challenges, a cellular state called endotoxin tolerance. In this study, we investigated the LPS-induced global glycoprotein expression changes of tolerant human monocytes and THP-1 cells to identify markers and glycoprotein targets capable to modulate the immunosuppressive state. Using hydrazide chemistry and LC-MS/MS analysis, we analyzed glycoprotein expression changes during a 48 h LPS time course. The cellular snapshots at different time points identified 1491 glycoproteins expressed by monocytes and THP-1 cells. Label-free quantitative analysis revealed transient or long-lasting LPS-induced expression changes of secreted or membrane-anchored glycoproteins derived from intracellular membrane coated organelles or from the plasma membrane. Monocytes and THP-1 cells demonstrated marked differences in glycoproteins differentially expressed in the tolerant state. Among the shared differentially expressed glycoproteins G protein-coupled receptor 84 (GPR84) was identified as being capable of modulating pro-inflammatory TNFα mRNA expression in the tolerant cell state when activated with its ligand Decanoic acid.
format article
author Mario M. Müller
Roland Lehmann
Tilman E. Klassert
Stella Reifenstein
Theresia Conrad
Christoph Moore
Anna Kuhn
Andrea Behnert
Reinhard Guthke
Dominik Driesch
Hortense Slevogt
author_facet Mario M. Müller
Roland Lehmann
Tilman E. Klassert
Stella Reifenstein
Theresia Conrad
Christoph Moore
Anna Kuhn
Andrea Behnert
Reinhard Guthke
Dominik Driesch
Hortense Slevogt
author_sort Mario M. Müller
title Global analysis of glycoproteins identifies markers of endotoxin tolerant monocytes and GPR84 as a modulator of TNFα expression
title_short Global analysis of glycoproteins identifies markers of endotoxin tolerant monocytes and GPR84 as a modulator of TNFα expression
title_full Global analysis of glycoproteins identifies markers of endotoxin tolerant monocytes and GPR84 as a modulator of TNFα expression
title_fullStr Global analysis of glycoproteins identifies markers of endotoxin tolerant monocytes and GPR84 as a modulator of TNFα expression
title_full_unstemmed Global analysis of glycoproteins identifies markers of endotoxin tolerant monocytes and GPR84 as a modulator of TNFα expression
title_sort global analysis of glycoproteins identifies markers of endotoxin tolerant monocytes and gpr84 as a modulator of tnfα expression
publisher Nature Portfolio
publishDate 2017
url https://doaj.org/article/7933dd5b970a4a05a24a95607778d7de
work_keys_str_mv AT mariommuller globalanalysisofglycoproteinsidentifiesmarkersofendotoxintolerantmonocytesandgpr84asamodulatoroftnfaexpression
AT rolandlehmann globalanalysisofglycoproteinsidentifiesmarkersofendotoxintolerantmonocytesandgpr84asamodulatoroftnfaexpression
AT tilmaneklassert globalanalysisofglycoproteinsidentifiesmarkersofendotoxintolerantmonocytesandgpr84asamodulatoroftnfaexpression
AT stellareifenstein globalanalysisofglycoproteinsidentifiesmarkersofendotoxintolerantmonocytesandgpr84asamodulatoroftnfaexpression
AT theresiaconrad globalanalysisofglycoproteinsidentifiesmarkersofendotoxintolerantmonocytesandgpr84asamodulatoroftnfaexpression
AT christophmoore globalanalysisofglycoproteinsidentifiesmarkersofendotoxintolerantmonocytesandgpr84asamodulatoroftnfaexpression
AT annakuhn globalanalysisofglycoproteinsidentifiesmarkersofendotoxintolerantmonocytesandgpr84asamodulatoroftnfaexpression
AT andreabehnert globalanalysisofglycoproteinsidentifiesmarkersofendotoxintolerantmonocytesandgpr84asamodulatoroftnfaexpression
AT reinhardguthke globalanalysisofglycoproteinsidentifiesmarkersofendotoxintolerantmonocytesandgpr84asamodulatoroftnfaexpression
AT dominikdriesch globalanalysisofglycoproteinsidentifiesmarkersofendotoxintolerantmonocytesandgpr84asamodulatoroftnfaexpression
AT hortenseslevogt globalanalysisofglycoproteinsidentifiesmarkersofendotoxintolerantmonocytesandgpr84asamodulatoroftnfaexpression
_version_ 1718395053469073408

Ejemplares similares