Lack of detection of XMRV in seminal plasma from HIV-1 infected men in The Netherlands.
<h4>Background</h4>Xenotropic murine leukaemia virus-related virus (XMRV) is a recently discovered human gammaretrovirus with yet unknown prevalence and transmission route(s). Its presence in prostate stromal fibroblasts and prostatic secretions suggests that XMRV might be sexually trans...
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2010
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oai:doaj.org-article:793c1288ac53447db3479d300d90804a2021-11-18T06:36:15ZLack of detection of XMRV in seminal plasma from HIV-1 infected men in The Netherlands.1932-620310.1371/journal.pone.0012040https://doaj.org/article/793c1288ac53447db3479d300d90804a2010-08-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/20706581/?tool=EBIhttps://doaj.org/toc/1932-6203<h4>Background</h4>Xenotropic murine leukaemia virus-related virus (XMRV) is a recently discovered human gammaretrovirus with yet unknown prevalence and transmission route(s). Its presence in prostate stromal fibroblasts and prostatic secretions suggests that XMRV might be sexually transmitted. We chose to study a compartment closely connected to the prostate, a location where XMRV was detected in independent studies. Seminal plasma samples from HIV-1 infected men were examined as they have an increased probability of acquiring sexually transmitted pathogens.<h4>Methodology/principal findings</h4>We studied the prevalence of XMRV in 93 seminal plasma samples of 54 HIV-1 infected men living in The Netherlands with a nested PCR amplification specifically targeting the XMRV gag gene. As a control for the presence and integrity of retrovirus particles, HIV-1 was amplified from the same samples with a PCR amplification targeting the env gene of the virus, or HIV-1 was quantified with a real-time PCR amplifying part of the pol gene.<h4>Conclusions/significance</h4>Although HIV-1 was amplified from 25% of the seminal plasma samples, no XMRV was detected, suggesting that either the prevalence of XMRV is very low in The Netherlands, or that XMRV is not naturally present in the seminal plasma.Marion CornelissenFokla ZorgdragerPetra BlomSuzanne JurriaansSjoerd ReppingElisabeth van LeeuwenMargreet BakkerBen BerkhoutAntoinette C van der KuylPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 5, Iss 8, p e12040 (2010) |
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Medicine R Science Q Marion Cornelissen Fokla Zorgdrager Petra Blom Suzanne Jurriaans Sjoerd Repping Elisabeth van Leeuwen Margreet Bakker Ben Berkhout Antoinette C van der Kuyl Lack of detection of XMRV in seminal plasma from HIV-1 infected men in The Netherlands. |
| description |
<h4>Background</h4>Xenotropic murine leukaemia virus-related virus (XMRV) is a recently discovered human gammaretrovirus with yet unknown prevalence and transmission route(s). Its presence in prostate stromal fibroblasts and prostatic secretions suggests that XMRV might be sexually transmitted. We chose to study a compartment closely connected to the prostate, a location where XMRV was detected in independent studies. Seminal plasma samples from HIV-1 infected men were examined as they have an increased probability of acquiring sexually transmitted pathogens.<h4>Methodology/principal findings</h4>We studied the prevalence of XMRV in 93 seminal plasma samples of 54 HIV-1 infected men living in The Netherlands with a nested PCR amplification specifically targeting the XMRV gag gene. As a control for the presence and integrity of retrovirus particles, HIV-1 was amplified from the same samples with a PCR amplification targeting the env gene of the virus, or HIV-1 was quantified with a real-time PCR amplifying part of the pol gene.<h4>Conclusions/significance</h4>Although HIV-1 was amplified from 25% of the seminal plasma samples, no XMRV was detected, suggesting that either the prevalence of XMRV is very low in The Netherlands, or that XMRV is not naturally present in the seminal plasma. |
| format |
article |
| author |
Marion Cornelissen Fokla Zorgdrager Petra Blom Suzanne Jurriaans Sjoerd Repping Elisabeth van Leeuwen Margreet Bakker Ben Berkhout Antoinette C van der Kuyl |
| author_facet |
Marion Cornelissen Fokla Zorgdrager Petra Blom Suzanne Jurriaans Sjoerd Repping Elisabeth van Leeuwen Margreet Bakker Ben Berkhout Antoinette C van der Kuyl |
| author_sort |
Marion Cornelissen |
| title |
Lack of detection of XMRV in seminal plasma from HIV-1 infected men in The Netherlands. |
| title_short |
Lack of detection of XMRV in seminal plasma from HIV-1 infected men in The Netherlands. |
| title_full |
Lack of detection of XMRV in seminal plasma from HIV-1 infected men in The Netherlands. |
| title_fullStr |
Lack of detection of XMRV in seminal plasma from HIV-1 infected men in The Netherlands. |
| title_full_unstemmed |
Lack of detection of XMRV in seminal plasma from HIV-1 infected men in The Netherlands. |
| title_sort |
lack of detection of xmrv in seminal plasma from hiv-1 infected men in the netherlands. |
| publisher |
Public Library of Science (PLoS) |
| publishDate |
2010 |
| url |
https://doaj.org/article/793c1288ac53447db3479d300d90804a |
| work_keys_str_mv |
AT marioncornelissen lackofdetectionofxmrvinseminalplasmafromhiv1infectedmeninthenetherlands AT foklazorgdrager lackofdetectionofxmrvinseminalplasmafromhiv1infectedmeninthenetherlands AT petrablom lackofdetectionofxmrvinseminalplasmafromhiv1infectedmeninthenetherlands AT suzannejurriaans lackofdetectionofxmrvinseminalplasmafromhiv1infectedmeninthenetherlands AT sjoerdrepping lackofdetectionofxmrvinseminalplasmafromhiv1infectedmeninthenetherlands AT elisabethvanleeuwen lackofdetectionofxmrvinseminalplasmafromhiv1infectedmeninthenetherlands AT margreetbakker lackofdetectionofxmrvinseminalplasmafromhiv1infectedmeninthenetherlands AT benberkhout lackofdetectionofxmrvinseminalplasmafromhiv1infectedmeninthenetherlands AT antoinettecvanderkuyl lackofdetectionofxmrvinseminalplasmafromhiv1infectedmeninthenetherlands |
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