Transcriptomic Profile of New Gene Markers Encoding Proteins Responsible for Structure of Porcine Ovarian Granulosa Cells

The extracellular matrix (ECM) in granulosa cells is functionally very important, and it is involved in many processes related to ovarian follicle growth and ovulation. The aim of this study was to describe the expression profile of genes within granulosa cells that are associated with extracellular...

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Autores principales:	Jakub Kulus, Magdalena Kulus, Wiesława Kranc, Karol Jopek, Maciej Zdun, Małgorzata Józkowiak, Jędrzej M. Jaśkowski, Hanna Piotrowska-Kempisty, Dorota Bukowska, Paweł Antosik, Paul Mozdziak, Bartosz Kempisty
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	porcine granulosa cells extracellular matrix gene expression profile folliculogenesis reproductive physiology Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/79475c0ed5e84f93a7df46b33492d1f2
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spelling	oai:doaj.org-article:79475c0ed5e84f93a7df46b33492d1f22021-11-25T16:48:11ZTranscriptomic Profile of New Gene Markers Encoding Proteins Responsible for Structure of Porcine Ovarian Granulosa Cells10.3390/biology101112142079-7737https://doaj.org/article/79475c0ed5e84f93a7df46b33492d1f22021-11-01T00:00:00Zhttps://www.mdpi.com/2079-7737/10/11/1214https://doaj.org/toc/2079-7737The extracellular matrix (ECM) in granulosa cells is functionally very important, and it is involved in many processes related to ovarian follicle growth and ovulation. The aim of this study was to describe the expression profile of genes within granulosa cells that are associated with extracellular matrix formation, intercellular signaling, and cell–cell fusion. The material for this study was ovaries of sexually mature pigs obtained from a commercial slaughterhouse. Laboratory-derived granulosa cells (GCs) from ovarian follicles were cultured in a primary in vitro culture model. The extracted genetic material (0, 48, 96, and 144 h) were subjected to microarray expression analysis. Among 81 genes, 66 showed increased expression and only 15 showed decreased expression were assigned to 7 gene ontology groups “extracellular matrix binding”, “extracellular matrix structural constituent”, “binding, bridging”, “cadherin binding”, “cell adhesion molecule binding”, “collagen binding” and “cadherin binding involved in cell-cell adhesion”. The 10 genes with the highest expression (POSTN, ITGA2, FN1, LAMB1, ITGB3, CHI3L1, PCOLCE2, CAV1, DCN, COL14A1) and 10 of the most down-regulated (SPP1, IRS1, CNTLN, TMPO, PAICS, ANK2, ADAM23, ABI3BP, DNAJB1, IGF1) were selected for further analysis. The results were validated by RT-qPCR. The current results may serve as preliminary data for further analyses using in vitro granulosa cell cultures in assisted reproduction technologies, studies of pathological processes in the ovary as well as in the use of the stemness potential of GCs.Jakub KulusMagdalena KulusWiesława KrancKarol JopekMaciej ZdunMałgorzata JózkowiakJędrzej M. JaśkowskiHanna Piotrowska-KempistyDorota BukowskaPaweł AntosikPaul MozdziakBartosz KempistyMDPI AGarticleporcine granulosa cellsextracellular matrixgene expression profilefolliculogenesisreproductive physiologyBiology (General)QH301-705.5ENBiology, Vol 10, Iss 1214, p 1214 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	porcine granulosa cells extracellular matrix gene expression profile folliculogenesis reproductive physiology Biology (General) QH301-705.5
spellingShingle	porcine granulosa cells extracellular matrix gene expression profile folliculogenesis reproductive physiology Biology (General) QH301-705.5 Jakub Kulus Magdalena Kulus Wiesława Kranc Karol Jopek Maciej Zdun Małgorzata Józkowiak Jędrzej M. Jaśkowski Hanna Piotrowska-Kempisty Dorota Bukowska Paweł Antosik Paul Mozdziak Bartosz Kempisty Transcriptomic Profile of New Gene Markers Encoding Proteins Responsible for Structure of Porcine Ovarian Granulosa Cells
description	The extracellular matrix (ECM) in granulosa cells is functionally very important, and it is involved in many processes related to ovarian follicle growth and ovulation. The aim of this study was to describe the expression profile of genes within granulosa cells that are associated with extracellular matrix formation, intercellular signaling, and cell–cell fusion. The material for this study was ovaries of sexually mature pigs obtained from a commercial slaughterhouse. Laboratory-derived granulosa cells (GCs) from ovarian follicles were cultured in a primary in vitro culture model. The extracted genetic material (0, 48, 96, and 144 h) were subjected to microarray expression analysis. Among 81 genes, 66 showed increased expression and only 15 showed decreased expression were assigned to 7 gene ontology groups “extracellular matrix binding”, “extracellular matrix structural constituent”, “binding, bridging”, “cadherin binding”, “cell adhesion molecule binding”, “collagen binding” and “cadherin binding involved in cell-cell adhesion”. The 10 genes with the highest expression (POSTN, ITGA2, FN1, LAMB1, ITGB3, CHI3L1, PCOLCE2, CAV1, DCN, COL14A1) and 10 of the most down-regulated (SPP1, IRS1, CNTLN, TMPO, PAICS, ANK2, ADAM23, ABI3BP, DNAJB1, IGF1) were selected for further analysis. The results were validated by RT-qPCR. The current results may serve as preliminary data for further analyses using in vitro granulosa cell cultures in assisted reproduction technologies, studies of pathological processes in the ovary as well as in the use of the stemness potential of GCs.
format	article
author	Jakub Kulus Magdalena Kulus Wiesława Kranc Karol Jopek Maciej Zdun Małgorzata Józkowiak Jędrzej M. Jaśkowski Hanna Piotrowska-Kempisty Dorota Bukowska Paweł Antosik Paul Mozdziak Bartosz Kempisty
author_facet	Jakub Kulus Magdalena Kulus Wiesława Kranc Karol Jopek Maciej Zdun Małgorzata Józkowiak Jędrzej M. Jaśkowski Hanna Piotrowska-Kempisty Dorota Bukowska Paweł Antosik Paul Mozdziak Bartosz Kempisty
author_sort	Jakub Kulus
title	Transcriptomic Profile of New Gene Markers Encoding Proteins Responsible for Structure of Porcine Ovarian Granulosa Cells
title_short	Transcriptomic Profile of New Gene Markers Encoding Proteins Responsible for Structure of Porcine Ovarian Granulosa Cells
title_full	Transcriptomic Profile of New Gene Markers Encoding Proteins Responsible for Structure of Porcine Ovarian Granulosa Cells
title_fullStr	Transcriptomic Profile of New Gene Markers Encoding Proteins Responsible for Structure of Porcine Ovarian Granulosa Cells
title_full_unstemmed	Transcriptomic Profile of New Gene Markers Encoding Proteins Responsible for Structure of Porcine Ovarian Granulosa Cells
title_sort	transcriptomic profile of new gene markers encoding proteins responsible for structure of porcine ovarian granulosa cells
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/79475c0ed5e84f93a7df46b33492d1f2
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Transcriptomic Profile of New Gene Markers Encoding Proteins Responsible for Structure of Porcine Ovarian Granulosa Cells

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