Draft whole genome sequence of groundnut stem rot fungus Athelia rolfsii revealing genetic architect of its pathogenicity and virulence

Abstract Groundnut (Arachis hypogaea L.) is an important oil seed crop having major biotic constraint in production due to stem rot disease caused by fungus, Athelia rolfsii causing 25–80% loss in productivity. As chemical and biological combating strategies of this fungus are not very effective, th...

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Autores principales:	M. A. Iquebal, Rukam S. Tomar, M. V. Parakhia, Deepak Singla, Sarika Jaiswal, V. M. Rathod, S. M. Padhiyar, Neeraj Kumar, Anil Rai, Dinesh Kumar
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Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/794f132c9141419289132b537a9fd4de
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spelling	oai:doaj.org-article:794f132c9141419289132b537a9fd4de2021-12-02T15:04:53ZDraft whole genome sequence of groundnut stem rot fungus Athelia rolfsii revealing genetic architect of its pathogenicity and virulence10.1038/s41598-017-05478-82045-2322https://doaj.org/article/794f132c9141419289132b537a9fd4de2017-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-05478-8https://doaj.org/toc/2045-2322Abstract Groundnut (Arachis hypogaea L.) is an important oil seed crop having major biotic constraint in production due to stem rot disease caused by fungus, Athelia rolfsii causing 25–80% loss in productivity. As chemical and biological combating strategies of this fungus are not very effective, thus genome sequencing can reveal virulence and pathogenicity related genes for better understanding of the host-parasite interaction. We report draft assembly of Athelia rolfsii genome of ~73 Mb having 8919 contigs. Annotation analysis revealed 16830 genes which are involved in fungicide resistance, virulence and pathogenicity along with putative effector and lethal genes. Secretome analysis revealed CAZY genes representing 1085 enzymatic genes, glycoside hydrolases, carbohydrate esterases, carbohydrate-binding modules, auxillary activities, glycosyl transferases and polysaccharide lyases. Repeat analysis revealed 11171 SSRs, LTR, GYPSY and COPIA elements. Comparative analysis with other existing ascomycotina genome predicted conserved domain family of WD40, CYP450, Pkinase and ABC transporter revealing insight of evolution of pathogenicity and virulence. This study would help in understanding pathogenicity and virulence at molecular level and development of new combating strategies. Such approach is imperative in endeavour of genome based solution in stem rot disease management leading to better productivity of groundnut crop in tropical region of world.M. A. IquebalRukam S. TomarM. V. ParakhiaDeepak SinglaSarika JaiswalV. M. RathodS. M. PadhiyarNeeraj KumarAnil RaiDinesh KumarNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-10 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q M. A. Iquebal Rukam S. Tomar M. V. Parakhia Deepak Singla Sarika Jaiswal V. M. Rathod S. M. Padhiyar Neeraj Kumar Anil Rai Dinesh Kumar Draft whole genome sequence of groundnut stem rot fungus Athelia rolfsii revealing genetic architect of its pathogenicity and virulence
description	Abstract Groundnut (Arachis hypogaea L.) is an important oil seed crop having major biotic constraint in production due to stem rot disease caused by fungus, Athelia rolfsii causing 25–80% loss in productivity. As chemical and biological combating strategies of this fungus are not very effective, thus genome sequencing can reveal virulence and pathogenicity related genes for better understanding of the host-parasite interaction. We report draft assembly of Athelia rolfsii genome of ~73 Mb having 8919 contigs. Annotation analysis revealed 16830 genes which are involved in fungicide resistance, virulence and pathogenicity along with putative effector and lethal genes. Secretome analysis revealed CAZY genes representing 1085 enzymatic genes, glycoside hydrolases, carbohydrate esterases, carbohydrate-binding modules, auxillary activities, glycosyl transferases and polysaccharide lyases. Repeat analysis revealed 11171 SSRs, LTR, GYPSY and COPIA elements. Comparative analysis with other existing ascomycotina genome predicted conserved domain family of WD40, CYP450, Pkinase and ABC transporter revealing insight of evolution of pathogenicity and virulence. This study would help in understanding pathogenicity and virulence at molecular level and development of new combating strategies. Such approach is imperative in endeavour of genome based solution in stem rot disease management leading to better productivity of groundnut crop in tropical region of world.
format	article
author	M. A. Iquebal Rukam S. Tomar M. V. Parakhia Deepak Singla Sarika Jaiswal V. M. Rathod S. M. Padhiyar Neeraj Kumar Anil Rai Dinesh Kumar
author_facet	M. A. Iquebal Rukam S. Tomar M. V. Parakhia Deepak Singla Sarika Jaiswal V. M. Rathod S. M. Padhiyar Neeraj Kumar Anil Rai Dinesh Kumar
author_sort	M. A. Iquebal
title	Draft whole genome sequence of groundnut stem rot fungus Athelia rolfsii revealing genetic architect of its pathogenicity and virulence
title_short	Draft whole genome sequence of groundnut stem rot fungus Athelia rolfsii revealing genetic architect of its pathogenicity and virulence
title_full	Draft whole genome sequence of groundnut stem rot fungus Athelia rolfsii revealing genetic architect of its pathogenicity and virulence
title_fullStr	Draft whole genome sequence of groundnut stem rot fungus Athelia rolfsii revealing genetic architect of its pathogenicity and virulence
title_full_unstemmed	Draft whole genome sequence of groundnut stem rot fungus Athelia rolfsii revealing genetic architect of its pathogenicity and virulence
title_sort	draft whole genome sequence of groundnut stem rot fungus athelia rolfsii revealing genetic architect of its pathogenicity and virulence
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/794f132c9141419289132b537a9fd4de
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Draft whole genome sequence of groundnut stem rot fungus Athelia rolfsii revealing genetic architect of its pathogenicity and virulence

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