Distinct Physiological Roles of Three Phospholipid:Diacylglycerol Acyltransferase Genes in Olive Fruit with Respect to Oil Accumulation and the Response to Abiotic Stress

Three different cDNA sequences, designated OepPDAT1-1, OepPDAT1-2, and OepPDAT2, encoding three phospholipid:diacylglycerol acyltransferases (PDAT) have been isolated from olive (Olea europaea cv. Picual). Sequence analysis showed the distinctive features typical of the PDAT family and together with...

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Autores principales:	M. Luisa Hernández, Samuele Moretti, M. Dolores Sicardo, Úrsula García, Ana Pérez, Luca Sebastiani, José M. Martínez-Rivas
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	Olea europaea olive fruit oil content PDAT gene expression triacylglycerol synthesis Plant culture SB1-1110
Acceso en línea:	https://doaj.org/article/794fe482f4c647928ed765752262714b
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spelling	oai:doaj.org-article:794fe482f4c647928ed765752262714b2021-11-12T04:45:19ZDistinct Physiological Roles of Three Phospholipid:Diacylglycerol Acyltransferase Genes in Olive Fruit with Respect to Oil Accumulation and the Response to Abiotic Stress1664-462X10.3389/fpls.2021.751959https://doaj.org/article/794fe482f4c647928ed765752262714b2021-11-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fpls.2021.751959/fullhttps://doaj.org/toc/1664-462XThree different cDNA sequences, designated OepPDAT1-1, OepPDAT1-2, and OepPDAT2, encoding three phospholipid:diacylglycerol acyltransferases (PDAT) have been isolated from olive (Olea europaea cv. Picual). Sequence analysis showed the distinctive features typical of the PDAT family and together with phylogenetic analysis indicated that they encode PDAT. Gene expression analysis in different olive tissues showed that transcript levels of these three PDAT genes are spatially and temporally regulated and suggested that, in addition to acyl-CoA:diacylglycerol acyltransferase, OePDAT1-1 may contribute to the biosynthesis of triacylglycerols in the seed, whereas OePDAT1-2 could be involved in the triacylglycerols content in the mesocarp and, therefore, in the olive oil. The relative contribution of PDAT and acyl-CoA:diacylglycerol acyltransferase enzymes to the triacylglycerols content in olive appears to be tissue-dependent. Furthermore, water regime, temperature, light, and wounding regulate PDAT genes at transcriptional level in the olive fruit mesocarp, indicating that PDAT could be involved in the response to abiotic stresses. Altogether, this study represents an advance in our knowledge on the regulation of oil accumulation in oil fruit.M. Luisa HernándezSamuele MorettiSamuele MorettiM. Dolores SicardoÚrsula GarcíaAna PérezLuca SebastianiJosé M. Martínez-RivasFrontiers Media S.A.articleOlea europaeaolive fruitoil contentPDATgene expressiontriacylglycerol synthesisPlant cultureSB1-1110ENFrontiers in Plant Science, Vol 12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Olea europaea olive fruit oil content PDAT gene expression triacylglycerol synthesis Plant culture SB1-1110
spellingShingle	Olea europaea olive fruit oil content PDAT gene expression triacylglycerol synthesis Plant culture SB1-1110 M. Luisa Hernández Samuele Moretti Samuele Moretti M. Dolores Sicardo Úrsula García Ana Pérez Luca Sebastiani José M. Martínez-Rivas Distinct Physiological Roles of Three Phospholipid:Diacylglycerol Acyltransferase Genes in Olive Fruit with Respect to Oil Accumulation and the Response to Abiotic Stress
description	Three different cDNA sequences, designated OepPDAT1-1, OepPDAT1-2, and OepPDAT2, encoding three phospholipid:diacylglycerol acyltransferases (PDAT) have been isolated from olive (Olea europaea cv. Picual). Sequence analysis showed the distinctive features typical of the PDAT family and together with phylogenetic analysis indicated that they encode PDAT. Gene expression analysis in different olive tissues showed that transcript levels of these three PDAT genes are spatially and temporally regulated and suggested that, in addition to acyl-CoA:diacylglycerol acyltransferase, OePDAT1-1 may contribute to the biosynthesis of triacylglycerols in the seed, whereas OePDAT1-2 could be involved in the triacylglycerols content in the mesocarp and, therefore, in the olive oil. The relative contribution of PDAT and acyl-CoA:diacylglycerol acyltransferase enzymes to the triacylglycerols content in olive appears to be tissue-dependent. Furthermore, water regime, temperature, light, and wounding regulate PDAT genes at transcriptional level in the olive fruit mesocarp, indicating that PDAT could be involved in the response to abiotic stresses. Altogether, this study represents an advance in our knowledge on the regulation of oil accumulation in oil fruit.
format	article
author	M. Luisa Hernández Samuele Moretti Samuele Moretti M. Dolores Sicardo Úrsula García Ana Pérez Luca Sebastiani José M. Martínez-Rivas
author_facet	M. Luisa Hernández Samuele Moretti Samuele Moretti M. Dolores Sicardo Úrsula García Ana Pérez Luca Sebastiani José M. Martínez-Rivas
author_sort	M. Luisa Hernández
title	Distinct Physiological Roles of Three Phospholipid:Diacylglycerol Acyltransferase Genes in Olive Fruit with Respect to Oil Accumulation and the Response to Abiotic Stress
title_short	Distinct Physiological Roles of Three Phospholipid:Diacylglycerol Acyltransferase Genes in Olive Fruit with Respect to Oil Accumulation and the Response to Abiotic Stress
title_full	Distinct Physiological Roles of Three Phospholipid:Diacylglycerol Acyltransferase Genes in Olive Fruit with Respect to Oil Accumulation and the Response to Abiotic Stress
title_fullStr	Distinct Physiological Roles of Three Phospholipid:Diacylglycerol Acyltransferase Genes in Olive Fruit with Respect to Oil Accumulation and the Response to Abiotic Stress
title_full_unstemmed	Distinct Physiological Roles of Three Phospholipid:Diacylglycerol Acyltransferase Genes in Olive Fruit with Respect to Oil Accumulation and the Response to Abiotic Stress
title_sort	distinct physiological roles of three phospholipid:diacylglycerol acyltransferase genes in olive fruit with respect to oil accumulation and the response to abiotic stress
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/794fe482f4c647928ed765752262714b
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Distinct Physiological Roles of Three Phospholipid:Diacylglycerol Acyltransferase Genes in Olive Fruit with Respect to Oil Accumulation and the Response to Abiotic Stress

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