In vitro synergy of isavuconazole in combination with colistin against Candida auris

Abstract The in vitro interactions of isavuconazole with colistin were evaluated against 15 clinical Candida auris isolates by a microdilution checkerboard technique based on the EUCAST reference method for antifungal susceptibility testing and by agar diffusion using isavuconazole gradient concentr...

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Autores principales: Patrick Schwarz, Anne-Laure Bidaud, Eric Dannaoui
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Publicado: Nature Portfolio 2020
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Acceso en línea:https://doaj.org/article/79501d61e37e46c0982f9ca343d90f6a
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spelling oai:doaj.org-article:79501d61e37e46c0982f9ca343d90f6a2021-12-02T12:33:04ZIn vitro synergy of isavuconazole in combination with colistin against Candida auris10.1038/s41598-020-78588-52045-2322https://doaj.org/article/79501d61e37e46c0982f9ca343d90f6a2020-12-01T00:00:00Zhttps://doi.org/10.1038/s41598-020-78588-5https://doaj.org/toc/2045-2322Abstract The in vitro interactions of isavuconazole with colistin were evaluated against 15 clinical Candida auris isolates by a microdilution checkerboard technique based on the EUCAST reference method for antifungal susceptibility testing and by agar diffusion using isavuconazole gradient concentration strips with or without colistin incorporated RPMI agar. Interpretation of the checkerboard results was done by the fractional inhibitory concentration index and by response surface analysis based on the Bliss model. By checkerboard, combination was synergistic for 93% of the isolates when interpretation of the data was done by fractional inhibitory concentration index, and for 80% of the isolates by response surface analysis interpretation. By agar diffusion test, although all MICs in combination decreased compared to isavuconazole alone, only 13% of the isolates met the definition of synergy. Essential agreement of EUCAST and gradient concentration strip MICs at +/− 2 log2 dilutions was 93.3%. Antagonistic interactions were never observed for any technique or interpretation model used.Patrick SchwarzAnne-Laure BidaudEric DannaouiNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 10, Iss 1, Pp 1-8 (2020)
institution DOAJ
collection DOAJ
language EN
topic Medicine
R
Science
Q
spellingShingle Medicine
R
Science
Q
Patrick Schwarz
Anne-Laure Bidaud
Eric Dannaoui
In vitro synergy of isavuconazole in combination with colistin against Candida auris
description Abstract The in vitro interactions of isavuconazole with colistin were evaluated against 15 clinical Candida auris isolates by a microdilution checkerboard technique based on the EUCAST reference method for antifungal susceptibility testing and by agar diffusion using isavuconazole gradient concentration strips with or without colistin incorporated RPMI agar. Interpretation of the checkerboard results was done by the fractional inhibitory concentration index and by response surface analysis based on the Bliss model. By checkerboard, combination was synergistic for 93% of the isolates when interpretation of the data was done by fractional inhibitory concentration index, and for 80% of the isolates by response surface analysis interpretation. By agar diffusion test, although all MICs in combination decreased compared to isavuconazole alone, only 13% of the isolates met the definition of synergy. Essential agreement of EUCAST and gradient concentration strip MICs at +/− 2 log2 dilutions was 93.3%. Antagonistic interactions were never observed for any technique or interpretation model used.
format article
author Patrick Schwarz
Anne-Laure Bidaud
Eric Dannaoui
author_facet Patrick Schwarz
Anne-Laure Bidaud
Eric Dannaoui
author_sort Patrick Schwarz
title In vitro synergy of isavuconazole in combination with colistin against Candida auris
title_short In vitro synergy of isavuconazole in combination with colistin against Candida auris
title_full In vitro synergy of isavuconazole in combination with colistin against Candida auris
title_fullStr In vitro synergy of isavuconazole in combination with colistin against Candida auris
title_full_unstemmed In vitro synergy of isavuconazole in combination with colistin against Candida auris
title_sort in vitro synergy of isavuconazole in combination with colistin against candida auris
publisher Nature Portfolio
publishDate 2020
url https://doaj.org/article/79501d61e37e46c0982f9ca343d90f6a
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AT annelaurebidaud invitrosynergyofisavuconazoleincombinationwithcolistinagainstcandidaauris
AT ericdannaoui invitrosynergyofisavuconazoleincombinationwithcolistinagainstcandidaauris
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