Unique distribution of ellagitannins in ripe strawberry fruit revealed by mass spectrometry imaging

Ellagitannins (ETs) are hydrolysable tannins composed of a polyol core, primarily glucose, which is esterified with hexahydroxydiphenic acid (HHDP), and in some cases, gallic acid. ETs are the major phenolic compounds found in strawberries and may contribute to the health-related properties of straw...

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Autor principal:	Hirofumi Enomoto
Formato:	article
Lenguaje:	EN
Publicado:	Elsevier 2021
Materias:	Ellagitannin Strawberry Distribution Mass spectrometry imaging Matrix-assisted laser desorption/ionization Liquid chromatography-mass spectrometry Nutrition. Foods and food supply TX341-641 Food processing and manufacture TP368-456
Acceso en línea:	https://doaj.org/article/79557024d8524c71ac76578ed25ffec2
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spelling	oai:doaj.org-article:79557024d8524c71ac76578ed25ffec22021-11-20T05:13:32ZUnique distribution of ellagitannins in ripe strawberry fruit revealed by mass spectrometry imaging2665-927110.1016/j.crfs.2021.11.006https://doaj.org/article/79557024d8524c71ac76578ed25ffec22021-01-01T00:00:00Zhttp://www.sciencedirect.com/science/article/pii/S2665927121000952https://doaj.org/toc/2665-9271Ellagitannins (ETs) are hydrolysable tannins composed of a polyol core, primarily glucose, which is esterified with hexahydroxydiphenic acid (HHDP), and in some cases, gallic acid. ETs are the major phenolic compounds found in strawberries and may contribute to the health-related properties of strawberries, because of their strong antioxidative activity. However, their distribution in the strawberry fruit remains unclear. In this study, matrix-assisted laser desorption/ionization-mass spectrometry imaging (MALDI–MSI) was used to visualize ETs in ripe strawberry fruits. Five peaks, corresponding to the m/z values of ET [M−H]− ions detected in the MALDI–MS spectrum of strawberry extracts, were identified as strictinin, pedunculagin, casuarictin, davuriicin M1, and an unknown ET using MALDI–tandem MS (MS/MS). In addition, liquid chromatography–electrospray ionization–MS/MS of the extracts revealed the presence of pedunculagin isomers and the unknown ET. Ion images of these five ETs were reconstructed using MALDI–MSI. Strictinin was widely distributed in and around the achene seed coats, while the other ETs were dispersed in and around the seed coats, and at the bottom of the receptacle; pedunculagin was distributed in the epidermis and pith, whereas casuarictin, the unknown ET, and davuriicin M1 were distributed in the pith. Moreover, MALDI–MSI of a casuarictin standard indicated that in-source fragmentation weakly affected the ion images. The results suggest that the distribution of ETs depends on the presence or absence of their constituents, namely galloyl units, HHDP, and bis-HHDP. To the best of my knowledge, this is the first report on the visualization of ETs in plant tissues using MSI, MALDI–MSI may be a useful tool for analyzing the distribution of ETs in the strawberry fruit.Hirofumi EnomotoElsevierarticleEllagitanninStrawberryDistributionMass spectrometry imagingMatrix-assisted laser desorption/ionizationLiquid chromatography-mass spectrometryNutrition. Foods and food supplyTX341-641Food processing and manufactureTP368-456ENCurrent Research in Food Science, Vol 4, Iss , Pp 821-828 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Ellagitannin Strawberry Distribution Mass spectrometry imaging Matrix-assisted laser desorption/ionization Liquid chromatography-mass spectrometry Nutrition. Foods and food supply TX341-641 Food processing and manufacture TP368-456
spellingShingle	Ellagitannin Strawberry Distribution Mass spectrometry imaging Matrix-assisted laser desorption/ionization Liquid chromatography-mass spectrometry Nutrition. Foods and food supply TX341-641 Food processing and manufacture TP368-456 Hirofumi Enomoto Unique distribution of ellagitannins in ripe strawberry fruit revealed by mass spectrometry imaging
description	Ellagitannins (ETs) are hydrolysable tannins composed of a polyol core, primarily glucose, which is esterified with hexahydroxydiphenic acid (HHDP), and in some cases, gallic acid. ETs are the major phenolic compounds found in strawberries and may contribute to the health-related properties of strawberries, because of their strong antioxidative activity. However, their distribution in the strawberry fruit remains unclear. In this study, matrix-assisted laser desorption/ionization-mass spectrometry imaging (MALDI–MSI) was used to visualize ETs in ripe strawberry fruits. Five peaks, corresponding to the m/z values of ET [M−H]− ions detected in the MALDI–MS spectrum of strawberry extracts, were identified as strictinin, pedunculagin, casuarictin, davuriicin M1, and an unknown ET using MALDI–tandem MS (MS/MS). In addition, liquid chromatography–electrospray ionization–MS/MS of the extracts revealed the presence of pedunculagin isomers and the unknown ET. Ion images of these five ETs were reconstructed using MALDI–MSI. Strictinin was widely distributed in and around the achene seed coats, while the other ETs were dispersed in and around the seed coats, and at the bottom of the receptacle; pedunculagin was distributed in the epidermis and pith, whereas casuarictin, the unknown ET, and davuriicin M1 were distributed in the pith. Moreover, MALDI–MSI of a casuarictin standard indicated that in-source fragmentation weakly affected the ion images. The results suggest that the distribution of ETs depends on the presence or absence of their constituents, namely galloyl units, HHDP, and bis-HHDP. To the best of my knowledge, this is the first report on the visualization of ETs in plant tissues using MSI, MALDI–MSI may be a useful tool for analyzing the distribution of ETs in the strawberry fruit.
format	article
author	Hirofumi Enomoto
author_facet	Hirofumi Enomoto
author_sort	Hirofumi Enomoto
title	Unique distribution of ellagitannins in ripe strawberry fruit revealed by mass spectrometry imaging
title_short	Unique distribution of ellagitannins in ripe strawberry fruit revealed by mass spectrometry imaging
title_full	Unique distribution of ellagitannins in ripe strawberry fruit revealed by mass spectrometry imaging
title_fullStr	Unique distribution of ellagitannins in ripe strawberry fruit revealed by mass spectrometry imaging
title_full_unstemmed	Unique distribution of ellagitannins in ripe strawberry fruit revealed by mass spectrometry imaging
title_sort	unique distribution of ellagitannins in ripe strawberry fruit revealed by mass spectrometry imaging
publisher	Elsevier
publishDate	2021
url	https://doaj.org/article/79557024d8524c71ac76578ed25ffec2
work_keys_str_mv	AT hirofumienomoto uniquedistributionofellagitanninsinripestrawberryfruitrevealedbymassspectrometryimaging
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Unique distribution of ellagitannins in ripe strawberry fruit revealed by mass spectrometry imaging

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