Effect of microbial immunostimulants on WSSV infection percentage and the expression of immune-related genes in white shrimp (Litopenaeus vannamei)

Background: The white spot syndrome virus (WSSV) causes high mortalities in aquaculture. The use of immunostimulants increases animal resistance. Objective: To evaluate the WSSV infection percentage and the immunostimulant effect of lactic acid bacteria and yeast (MI= microbial immunostimulants) on...

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Autores principales:	Jesús A. Fierro Coronado, Antonio Luna González, Carlos J. Caceres Martínez, Píndaro Álvarez Ruiz, Ruth Escamilla-Montes, Héctor A. González Ocampo, Viridiana Peraza-Gómez
Formato:	article
Lenguaje:	EN
Publicado:	Universidad de Antioquia 2019
Materias:	candida parapsilosis gene expression immune system litopenaeus vannamei pediococcus parvulus shrimp white shrimp Animal culture SF1-1100
Acceso en línea:	https://doaj.org/article/7961b8360340458285453a3555ce516d
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spelling	oai:doaj.org-article:7961b8360340458285453a3555ce516d2021-12-01T18:49:32ZEffect of microbial immunostimulants on WSSV infection percentage and the expression of immune-related genes in white shrimp (Litopenaeus vannamei)2256-295810.17533/udea.rccp.v32n3a07https://doaj.org/article/7961b8360340458285453a3555ce516d2019-07-01T00:00:00Zhttps://revistas.udea.edu.co/index.php/rccp/article/view/334865https://doaj.org/toc/2256-2958Background: The white spot syndrome virus (WSSV) causes high mortalities in aquaculture. The use of immunostimulants increases animal resistance. Objective: To evaluate the WSSV infection percentage and the immunostimulant effect of lactic acid bacteria and yeast (MI= microbial immunostimulants) on WSSV infected Litopenaeus vannamei. Methods: A bioassay was performed for 33 d, with treatments in triplicate. The MI was added to the feed at 8.5 mg/kg feed and offered to shrimp (9.9 ± 3.1 g) daily, every 2 days, or every 3 days. Shrimp were infected with WSSV at 9 and 19 days. The expression of four immune system-related genes was studied using qRT-PCR. Results: No significant differences were observed in growth and survival among treatments. At the end of the bioassay, WSSV infection percentage (low viral load) decreased 8.3 and 25% in treatments III and IV as compared to the control group. Treatments with MI showed significant differences in the relative expression of LvToll, transglutaminase, and prophenoloxidase genes when MI was offered daily as compared to the control group. The MI did not regulate the expression of the superoxide dismutase gene. The WSSV infection percentage decreased when feed with MI was offered every 3 d. Conclusion: The MI decrease WSSV infection percentage in L. vannamei infected with low viral load when it is offered every three days. The MI up-regulates LvToll, Tgase, and proPO genes when it is offered daily. Further research is needed regarding prophylactic treatment with microbial immunostimulants against WSSV in commercial shrimp farms.Jesús A. Fierro CoronadoAntonio Luna GonzálezCarlos J. Caceres MartínezPíndaro Álvarez RuizRuth Escamilla-MontesHéctor A. González OcampoViridiana Peraza-GómezUniversidad de Antioquiaarticlecandida parapsilosisgene expressionimmune systemlitopenaeus vannameipediococcus parvulusshrimpwhite shrimpAnimal cultureSF1-1100ENRevista Colombiana de Ciencias Pecuarias, Vol 32, Iss 3, Pp 221-231 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	candida parapsilosis gene expression immune system litopenaeus vannamei pediococcus parvulus shrimp white shrimp Animal culture SF1-1100
spellingShingle	candida parapsilosis gene expression immune system litopenaeus vannamei pediococcus parvulus shrimp white shrimp Animal culture SF1-1100 Jesús A. Fierro Coronado Antonio Luna González Carlos J. Caceres Martínez Píndaro Álvarez Ruiz Ruth Escamilla-Montes Héctor A. González Ocampo Viridiana Peraza-Gómez Effect of microbial immunostimulants on WSSV infection percentage and the expression of immune-related genes in white shrimp (Litopenaeus vannamei)
description	Background: The white spot syndrome virus (WSSV) causes high mortalities in aquaculture. The use of immunostimulants increases animal resistance. Objective: To evaluate the WSSV infection percentage and the immunostimulant effect of lactic acid bacteria and yeast (MI= microbial immunostimulants) on WSSV infected Litopenaeus vannamei. Methods: A bioassay was performed for 33 d, with treatments in triplicate. The MI was added to the feed at 8.5 mg/kg feed and offered to shrimp (9.9 ± 3.1 g) daily, every 2 days, or every 3 days. Shrimp were infected with WSSV at 9 and 19 days. The expression of four immune system-related genes was studied using qRT-PCR. Results: No significant differences were observed in growth and survival among treatments. At the end of the bioassay, WSSV infection percentage (low viral load) decreased 8.3 and 25% in treatments III and IV as compared to the control group. Treatments with MI showed significant differences in the relative expression of LvToll, transglutaminase, and prophenoloxidase genes when MI was offered daily as compared to the control group. The MI did not regulate the expression of the superoxide dismutase gene. The WSSV infection percentage decreased when feed with MI was offered every 3 d. Conclusion: The MI decrease WSSV infection percentage in L. vannamei infected with low viral load when it is offered every three days. The MI up-regulates LvToll, Tgase, and proPO genes when it is offered daily. Further research is needed regarding prophylactic treatment with microbial immunostimulants against WSSV in commercial shrimp farms.
format	article
author	Jesús A. Fierro Coronado Antonio Luna González Carlos J. Caceres Martínez Píndaro Álvarez Ruiz Ruth Escamilla-Montes Héctor A. González Ocampo Viridiana Peraza-Gómez
author_facet	Jesús A. Fierro Coronado Antonio Luna González Carlos J. Caceres Martínez Píndaro Álvarez Ruiz Ruth Escamilla-Montes Héctor A. González Ocampo Viridiana Peraza-Gómez
author_sort	Jesús A. Fierro Coronado
title	Effect of microbial immunostimulants on WSSV infection percentage and the expression of immune-related genes in white shrimp (Litopenaeus vannamei)
title_short	Effect of microbial immunostimulants on WSSV infection percentage and the expression of immune-related genes in white shrimp (Litopenaeus vannamei)
title_full	Effect of microbial immunostimulants on WSSV infection percentage and the expression of immune-related genes in white shrimp (Litopenaeus vannamei)
title_fullStr	Effect of microbial immunostimulants on WSSV infection percentage and the expression of immune-related genes in white shrimp (Litopenaeus vannamei)
title_full_unstemmed	Effect of microbial immunostimulants on WSSV infection percentage and the expression of immune-related genes in white shrimp (Litopenaeus vannamei)
title_sort	effect of microbial immunostimulants on wssv infection percentage and the expression of immune-related genes in white shrimp (litopenaeus vannamei)
publisher	Universidad de Antioquia
publishDate	2019
url	https://doaj.org/article/7961b8360340458285453a3555ce516d
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Effect of microbial immunostimulants on WSSV infection percentage and the expression of immune-related genes in white shrimp (Litopenaeus vannamei)

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