Weigh Biomaterials by Quantifying Species-specific DNA with Real-time PCR

Abstract What’s on the label is not what’s in the bottle, from food products to herbal medicinal products (HMPs), economically-motivated biomaterials adulteration is a long-term problem affecting the food and drug industry. Accurate identification of the biomaterial ingredients in processed commodit...

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Autores principales:	Rong Chen, Jiayu Wang, Yi Yuan, Yun Deng, Xianrong Lai, Feng Du, Juan Dong, Xin Huang, Xin Cui, Zhuo Tang
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/79650c5db58b4a03b4fc27299dbdd8ce
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spelling	oai:doaj.org-article:79650c5db58b4a03b4fc27299dbdd8ce2021-12-02T12:32:38ZWeigh Biomaterials by Quantifying Species-specific DNA with Real-time PCR10.1038/s41598-017-05083-92045-2322https://doaj.org/article/79650c5db58b4a03b4fc27299dbdd8ce2017-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-05083-9https://doaj.org/toc/2045-2322Abstract What’s on the label is not what’s in the bottle, from food products to herbal medicinal products (HMPs), economically-motivated biomaterials adulteration is a long-term problem affecting the food and drug industry. Accurate identification of the biomaterial ingredients in processed commodities is highly desirable. In this field, DNA-based techniques have proved to be powerful tools to overcome qualitative challenges. However, is it possible to quantify the weight of biological materials with PCR? Therefore, a basic scientific question needs to be answered: what’s the relationship between DNA content and the mass of biological materials? Is DNA content directly proportional to the mass of biological materials as most of the researchers previously thought? In this study, we firstly found that there exists a linear relation between DNA contents and the weight of biomaterials indeed when the analytical practices are fully controlled. In this case, the mass of targeted biomaterials in the highly processed commercial products can also be calculated by quantifying the species-specific DNA through classic real-time PCR with a good reproducibility.Rong ChenJiayu WangYi YuanYun DengXianrong LaiFeng DuJuan DongXin HuangXin CuiZhuo TangNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-7 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Rong Chen Jiayu Wang Yi Yuan Yun Deng Xianrong Lai Feng Du Juan Dong Xin Huang Xin Cui Zhuo Tang Weigh Biomaterials by Quantifying Species-specific DNA with Real-time PCR
description	Abstract What’s on the label is not what’s in the bottle, from food products to herbal medicinal products (HMPs), economically-motivated biomaterials adulteration is a long-term problem affecting the food and drug industry. Accurate identification of the biomaterial ingredients in processed commodities is highly desirable. In this field, DNA-based techniques have proved to be powerful tools to overcome qualitative challenges. However, is it possible to quantify the weight of biological materials with PCR? Therefore, a basic scientific question needs to be answered: what’s the relationship between DNA content and the mass of biological materials? Is DNA content directly proportional to the mass of biological materials as most of the researchers previously thought? In this study, we firstly found that there exists a linear relation between DNA contents and the weight of biomaterials indeed when the analytical practices are fully controlled. In this case, the mass of targeted biomaterials in the highly processed commercial products can also be calculated by quantifying the species-specific DNA through classic real-time PCR with a good reproducibility.
format	article
author	Rong Chen Jiayu Wang Yi Yuan Yun Deng Xianrong Lai Feng Du Juan Dong Xin Huang Xin Cui Zhuo Tang
author_facet	Rong Chen Jiayu Wang Yi Yuan Yun Deng Xianrong Lai Feng Du Juan Dong Xin Huang Xin Cui Zhuo Tang
author_sort	Rong Chen
title	Weigh Biomaterials by Quantifying Species-specific DNA with Real-time PCR
title_short	Weigh Biomaterials by Quantifying Species-specific DNA with Real-time PCR
title_full	Weigh Biomaterials by Quantifying Species-specific DNA with Real-time PCR
title_fullStr	Weigh Biomaterials by Quantifying Species-specific DNA with Real-time PCR
title_full_unstemmed	Weigh Biomaterials by Quantifying Species-specific DNA with Real-time PCR
title_sort	weigh biomaterials by quantifying species-specific dna with real-time pcr
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/79650c5db58b4a03b4fc27299dbdd8ce
work_keys_str_mv	AT rongchen weighbiomaterialsbyquantifyingspeciesspecificdnawithrealtimepcr AT jiayuwang weighbiomaterialsbyquantifyingspeciesspecificdnawithrealtimepcr AT yiyuan weighbiomaterialsbyquantifyingspeciesspecificdnawithrealtimepcr AT yundeng weighbiomaterialsbyquantifyingspeciesspecificdnawithrealtimepcr AT xianronglai weighbiomaterialsbyquantifyingspeciesspecificdnawithrealtimepcr AT fengdu weighbiomaterialsbyquantifyingspeciesspecificdnawithrealtimepcr AT juandong weighbiomaterialsbyquantifyingspeciesspecificdnawithrealtimepcr AT xinhuang weighbiomaterialsbyquantifyingspeciesspecificdnawithrealtimepcr AT xincui weighbiomaterialsbyquantifyingspeciesspecificdnawithrealtimepcr AT zhuotang weighbiomaterialsbyquantifyingspeciesspecificdnawithrealtimepcr
_version_	1718393988034068480

Weigh Biomaterials by Quantifying Species-specific DNA with Real-time PCR

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