Overexpression of the PAP1 transcription factor reveals a complex regulation of flavonoid and phenylpropanoid metabolism in Nicotiana tabacum plants attacked by Spodoptera litura.

Anthocyanin pigments and associated flavonoids have demonstrated antioxidant properties and benefits for human health. Consequently, current plant bioengineers have focused on how to modify flavonoid metabolism in plants. Most of that research, however, does not consider the role of natural biotic s...

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Autores principales:	Tomoko Mitsunami, Masahiro Nishihara, Ivan Galis, Kabir Md Alamgir, Yuko Hojo, Kohei Fujita, Nobuhiro Sasaki, Keichiro Nemoto, Tatsuya Sawasaki, Gen-ichiro Arimura
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2014
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/7965793661d54a8692c7fd72af577ccd
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spelling	oai:doaj.org-article:7965793661d54a8692c7fd72af577ccd2021-11-25T05:58:47ZOverexpression of the PAP1 transcription factor reveals a complex regulation of flavonoid and phenylpropanoid metabolism in Nicotiana tabacum plants attacked by Spodoptera litura.1932-620310.1371/journal.pone.0108849https://doaj.org/article/7965793661d54a8692c7fd72af577ccd2014-01-01T00:00:00Zhttps://doi.org/10.1371/journal.pone.0108849https://doaj.org/toc/1932-6203Anthocyanin pigments and associated flavonoids have demonstrated antioxidant properties and benefits for human health. Consequently, current plant bioengineers have focused on how to modify flavonoid metabolism in plants. Most of that research, however, does not consider the role of natural biotic stresses (e.g., herbivore attack). To understand the influence of herbivore attack on the metabolic engineering of flavonoids, we examined tobacco plants overexpressing the Arabidopsis PAP1 gene (encoding an MYB transcription factor), which accumulated anthocyanin pigments and other flavonoids/phenylpropanoids. In comparison to wild-type and control plants, transgenic plants exhibited greater resistance to Spodoptera litura. Moreover, herbivory suppressed the PAP1-induced increase of transcripts of flavonoid/phenylpropanoid biosynthetic genes (e.g., F3H) and the subsequent accumulation of these genes' metabolites, despite the unaltered PAP1 mRNA levels after herbivory. The instances of down-regulation were independent of the signaling pathways mediated by defense-related jasmonates but were relevant to the levels of PAP1-induced and herbivory-suppressed transcription factors, An1a and An1b. Although initially F3H transcripts were suppressed by herbivory, after the S. litura feeding was interrupted, F3H transcripts increased. We hypothesize that in transgenic plants responding to herbivory, there is a complex mechanism regulating enriched flavonoid/phenylpropanoid compounds, via biotic stress signals.Tomoko MitsunamiMasahiro NishiharaIvan GalisKabir Md AlamgirYuko HojoKohei FujitaNobuhiro SasakiKeichiro NemotoTatsuya SawasakiGen-ichiro ArimuraPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 9, Iss 9, p e108849 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Tomoko Mitsunami Masahiro Nishihara Ivan Galis Kabir Md Alamgir Yuko Hojo Kohei Fujita Nobuhiro Sasaki Keichiro Nemoto Tatsuya Sawasaki Gen-ichiro Arimura Overexpression of the PAP1 transcription factor reveals a complex regulation of flavonoid and phenylpropanoid metabolism in Nicotiana tabacum plants attacked by Spodoptera litura.
description	Anthocyanin pigments and associated flavonoids have demonstrated antioxidant properties and benefits for human health. Consequently, current plant bioengineers have focused on how to modify flavonoid metabolism in plants. Most of that research, however, does not consider the role of natural biotic stresses (e.g., herbivore attack). To understand the influence of herbivore attack on the metabolic engineering of flavonoids, we examined tobacco plants overexpressing the Arabidopsis PAP1 gene (encoding an MYB transcription factor), which accumulated anthocyanin pigments and other flavonoids/phenylpropanoids. In comparison to wild-type and control plants, transgenic plants exhibited greater resistance to Spodoptera litura. Moreover, herbivory suppressed the PAP1-induced increase of transcripts of flavonoid/phenylpropanoid biosynthetic genes (e.g., F3H) and the subsequent accumulation of these genes' metabolites, despite the unaltered PAP1 mRNA levels after herbivory. The instances of down-regulation were independent of the signaling pathways mediated by defense-related jasmonates but were relevant to the levels of PAP1-induced and herbivory-suppressed transcription factors, An1a and An1b. Although initially F3H transcripts were suppressed by herbivory, after the S. litura feeding was interrupted, F3H transcripts increased. We hypothesize that in transgenic plants responding to herbivory, there is a complex mechanism regulating enriched flavonoid/phenylpropanoid compounds, via biotic stress signals.
format	article
author	Tomoko Mitsunami Masahiro Nishihara Ivan Galis Kabir Md Alamgir Yuko Hojo Kohei Fujita Nobuhiro Sasaki Keichiro Nemoto Tatsuya Sawasaki Gen-ichiro Arimura
author_facet	Tomoko Mitsunami Masahiro Nishihara Ivan Galis Kabir Md Alamgir Yuko Hojo Kohei Fujita Nobuhiro Sasaki Keichiro Nemoto Tatsuya Sawasaki Gen-ichiro Arimura
author_sort	Tomoko Mitsunami
title	Overexpression of the PAP1 transcription factor reveals a complex regulation of flavonoid and phenylpropanoid metabolism in Nicotiana tabacum plants attacked by Spodoptera litura.
title_short	Overexpression of the PAP1 transcription factor reveals a complex regulation of flavonoid and phenylpropanoid metabolism in Nicotiana tabacum plants attacked by Spodoptera litura.
title_full	Overexpression of the PAP1 transcription factor reveals a complex regulation of flavonoid and phenylpropanoid metabolism in Nicotiana tabacum plants attacked by Spodoptera litura.
title_fullStr	Overexpression of the PAP1 transcription factor reveals a complex regulation of flavonoid and phenylpropanoid metabolism in Nicotiana tabacum plants attacked by Spodoptera litura.
title_full_unstemmed	Overexpression of the PAP1 transcription factor reveals a complex regulation of flavonoid and phenylpropanoid metabolism in Nicotiana tabacum plants attacked by Spodoptera litura.
title_sort	overexpression of the pap1 transcription factor reveals a complex regulation of flavonoid and phenylpropanoid metabolism in nicotiana tabacum plants attacked by spodoptera litura.
publisher	Public Library of Science (PLoS)
publishDate	2014
url	https://doaj.org/article/7965793661d54a8692c7fd72af577ccd
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Overexpression of the PAP1 transcription factor reveals a complex regulation of flavonoid and phenylpropanoid metabolism in Nicotiana tabacum plants attacked by Spodoptera litura.

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