Antiviral Properties of Water Extracts of Mycelium <i>Inonotus rheades</i> (Pers.) P. Karst. (1882) against the Virus of Tick-Borne Encephalitis Virus <i>in vitro</i>

Background. Tick-borne encephalitis virus is dangerous and widespread pathogen that is transmitted to humans through the bites of hard ticks. Wild fungi, such as xylotrophic basidiomycetes, are widely used in traditional medicine to treat the infectious diseases and are promising natural sources of...

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Autores principales:	M. A. Khasnatinov, T. G. Gornostai, I. S. Solovarov, M. S. Polyakova, G. A. Danchinova, G. V. Borovskii
Formato:	article
Lenguaje:	RU
Publicado:	Scientific Сentre for Family Health and Human Reproduction Problems 2021
Materias:	antiviral activity inonotus rheades tick-borne encephalitis virus birch wood Science Q
Acceso en línea:	https://doaj.org/article/79671431ee014d36a098312e8e02bf77
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spelling	oai:doaj.org-article:79671431ee014d36a098312e8e02bf772021-11-23T06:14:45ZAntiviral Properties of Water Extracts of Mycelium <i>Inonotus rheades</i> (Pers.) P. Karst. (1882) against the Virus of Tick-Borne Encephalitis Virus <i>in vitro</i>2541-94202587-959610.29413/ABS.2021-6.1.8https://doaj.org/article/79671431ee014d36a098312e8e02bf772021-04-01T00:00:00Zhttps://www.actabiomedica.ru/jour/article/view/2615https://doaj.org/toc/2541-9420https://doaj.org/toc/2587-9596Background. Tick-borne encephalitis virus is dangerous and widespread pathogen that is transmitted to humans through the bites of hard ticks. Wild fungi, such as xylotrophic basidiomycetes, are widely used in traditional medicine to treat the infectious diseases and are promising natural sources of new antiviral agents. It was previously shown that aqueous extracts from the mycelium of the Inonotus rheades (Pers.) P. Karst. (1882) fungus exhibit significant antiviral activity against tick-borne encephalitis virus, however, the mechanisms of this activity remain unclear.Aim. To analyze the relationship between the virucidal properties of I. rheades extract and the substrate on which the cultivation was carried out.Materials and methods. The mycelium was grown either in a standard liquid medium with wort or on wooden disks from birch. Extracts of water-soluble polysaccharides were prepared from both mycelium samples. The concentration of infectious tick-borne encephalitis virus was determined using the method of titration of plaque-forming components (PFU). Approximately 30 000 PFU of tick-borne encephalitis virus was mixed with an equal volume of corresponding I. rheades extract at concentration of 8 mg/mL and incubated for 30 min at 37 °C. Afterwards, the residual infectivity of tick-borne encephalitis virus was determined using the identical virus sample incubated with sterile water as a reference.Results. It was found that treatment of tick-borne encephalitis virus with extracts from I. rheades mycelium resulted in inhibition of the infectivity of the virus in the cell culture. However, the same strain of I. rheades, grown on medium with wort, did not exhibit antiviral properties.Conclusions. Virucidal substances are likely to be not the main metabolites of the mycelium of I. rheades, but are rather metabolized wood polysaccharides. Further research is needed to more accurately identify the active ingredients and assess their antiviral activity.M. A. KhasnatinovT. G. GornostaiI. S. SolovarovM. S. PolyakovaG. A. DanchinovaG. V. BorovskiiScientific Сentre for Family Health and Human Reproduction Problemsarticleantiviral activityinonotus rheadestick-borne encephalitis virusbirch woodScienceQRUActa Biomedica Scientifica, Vol 6, Iss 1, Pp 55-59 (2021)
institution	DOAJ
collection	DOAJ
language	RU
topic	antiviral activity inonotus rheades tick-borne encephalitis virus birch wood Science Q
spellingShingle	antiviral activity inonotus rheades tick-borne encephalitis virus birch wood Science Q M. A. Khasnatinov T. G. Gornostai I. S. Solovarov M. S. Polyakova G. A. Danchinova G. V. Borovskii Antiviral Properties of Water Extracts of Mycelium <i>Inonotus rheades</i> (Pers.) P. Karst. (1882) against the Virus of Tick-Borne Encephalitis Virus <i>in vitro</i>
description	Background. Tick-borne encephalitis virus is dangerous and widespread pathogen that is transmitted to humans through the bites of hard ticks. Wild fungi, such as xylotrophic basidiomycetes, are widely used in traditional medicine to treat the infectious diseases and are promising natural sources of new antiviral agents. It was previously shown that aqueous extracts from the mycelium of the Inonotus rheades (Pers.) P. Karst. (1882) fungus exhibit significant antiviral activity against tick-borne encephalitis virus, however, the mechanisms of this activity remain unclear.Aim. To analyze the relationship between the virucidal properties of I. rheades extract and the substrate on which the cultivation was carried out.Materials and methods. The mycelium was grown either in a standard liquid medium with wort or on wooden disks from birch. Extracts of water-soluble polysaccharides were prepared from both mycelium samples. The concentration of infectious tick-borne encephalitis virus was determined using the method of titration of plaque-forming components (PFU). Approximately 30 000 PFU of tick-borne encephalitis virus was mixed with an equal volume of corresponding I. rheades extract at concentration of 8 mg/mL and incubated for 30 min at 37 °C. Afterwards, the residual infectivity of tick-borne encephalitis virus was determined using the identical virus sample incubated with sterile water as a reference.Results. It was found that treatment of tick-borne encephalitis virus with extracts from I. rheades mycelium resulted in inhibition of the infectivity of the virus in the cell culture. However, the same strain of I. rheades, grown on medium with wort, did not exhibit antiviral properties.Conclusions. Virucidal substances are likely to be not the main metabolites of the mycelium of I. rheades, but are rather metabolized wood polysaccharides. Further research is needed to more accurately identify the active ingredients and assess their antiviral activity.
format	article
author	M. A. Khasnatinov T. G. Gornostai I. S. Solovarov M. S. Polyakova G. A. Danchinova G. V. Borovskii
author_facet	M. A. Khasnatinov T. G. Gornostai I. S. Solovarov M. S. Polyakova G. A. Danchinova G. V. Borovskii
author_sort	M. A. Khasnatinov
title	Antiviral Properties of Water Extracts of Mycelium <i>Inonotus rheades</i> (Pers.) P. Karst. (1882) against the Virus of Tick-Borne Encephalitis Virus <i>in vitro</i>
title_short	Antiviral Properties of Water Extracts of Mycelium <i>Inonotus rheades</i> (Pers.) P. Karst. (1882) against the Virus of Tick-Borne Encephalitis Virus <i>in vitro</i>
title_full	Antiviral Properties of Water Extracts of Mycelium <i>Inonotus rheades</i> (Pers.) P. Karst. (1882) against the Virus of Tick-Borne Encephalitis Virus <i>in vitro</i>
title_fullStr	Antiviral Properties of Water Extracts of Mycelium <i>Inonotus rheades</i> (Pers.) P. Karst. (1882) against the Virus of Tick-Borne Encephalitis Virus <i>in vitro</i>
title_full_unstemmed	Antiviral Properties of Water Extracts of Mycelium <i>Inonotus rheades</i> (Pers.) P. Karst. (1882) against the Virus of Tick-Borne Encephalitis Virus <i>in vitro</i>
title_sort	antiviral properties of water extracts of mycelium <i>inonotus rheades</i> (pers.) p. karst. (1882) against the virus of tick-borne encephalitis virus <i>in vitro</i>
publisher	Scientific Сentre for Family Health and Human Reproduction Problems
publishDate	2021
url	https://doaj.org/article/79671431ee014d36a098312e8e02bf77
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Antiviral Properties of Water Extracts of Mycelium <i>Inonotus rheades</i> (Pers.) P. Karst. (1882) against the Virus of Tick-Borne Encephalitis Virus <i>in vitro</i>

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