Descemet’s Membrane Supports Corneal Endothelial Cell Regeneration in Rabbits

Abstract Descemet’s membrane (DM) helps maintain phenotype and function of corneal endothelial cells under physiological conditions, while little is known about the function of DM in corneal endothelial wound healing process. In the current study, we performed in vivo rabbit corneal endothelial cell...

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Autores principales:	Jingyao Chen, Zhiyuan Li, Liying Zhang, Shangkun Ou, Yanzi Wang, Xin He, Dulei Zou, Changkai Jia, Qianqian Hu, Shu Yang, Xian Li, Juan Li, Junqi Wang, Huimin Sun, Yongxiong Chen, Ying-Ting Zhu, Scheffer C. G. Tseng, Zuguo Liu, Wei Li
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/797519f7b29a44628ed3c0dc83b2fcb1
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spelling	oai:doaj.org-article:797519f7b29a44628ed3c0dc83b2fcb12021-12-02T16:07:04ZDescemet’s Membrane Supports Corneal Endothelial Cell Regeneration in Rabbits10.1038/s41598-017-07557-22045-2322https://doaj.org/article/797519f7b29a44628ed3c0dc83b2fcb12017-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-07557-2https://doaj.org/toc/2045-2322Abstract Descemet’s membrane (DM) helps maintain phenotype and function of corneal endothelial cells under physiological conditions, while little is known about the function of DM in corneal endothelial wound healing process. In the current study, we performed in vivo rabbit corneal endothelial cell (CEC) injury via CEC scraping, in which DM remained intact after CECs removal, or via DM stripping, in which DM was removed together with CECs. We found rabbit corneas in the CEC scraping group healed with transparency restoration, while there was posterior fibrosis tissue formation in the corneas after DM stripping on day 14. Following CEC scraping on day 3, cells that had migrated toward the central cornea underwent a transient fibrotic endothelial-mesenchymal transition (EMT) which was reversed back to an endothelial phenotype on day 14. However, in the corneas injured via DM stripping, most of the cells in the posterior fibrosis tissue did not originate from the corneal endothelium, and they maintained fibroblastic phenotype on day 14. We concluded that corneal endothelial wound healing in rabbits has different outcomes depending upon the presence or absence of Descemet’s membrane. Descemet’s membrane supports corneal endothelial cell regeneration in rabbits after endothelial injury.Jingyao ChenZhiyuan LiLiying ZhangShangkun OuYanzi WangXin HeDulei ZouChangkai JiaQianqian HuShu YangXian LiJuan LiJunqi WangHuimin SunYongxiong ChenYing-Ting ZhuScheffer C. G. TsengZuguo LiuWei LiNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-13 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Jingyao Chen Zhiyuan Li Liying Zhang Shangkun Ou Yanzi Wang Xin He Dulei Zou Changkai Jia Qianqian Hu Shu Yang Xian Li Juan Li Junqi Wang Huimin Sun Yongxiong Chen Ying-Ting Zhu Scheffer C. G. Tseng Zuguo Liu Wei Li Descemet’s Membrane Supports Corneal Endothelial Cell Regeneration in Rabbits
description	Abstract Descemet’s membrane (DM) helps maintain phenotype and function of corneal endothelial cells under physiological conditions, while little is known about the function of DM in corneal endothelial wound healing process. In the current study, we performed in vivo rabbit corneal endothelial cell (CEC) injury via CEC scraping, in which DM remained intact after CECs removal, or via DM stripping, in which DM was removed together with CECs. We found rabbit corneas in the CEC scraping group healed with transparency restoration, while there was posterior fibrosis tissue formation in the corneas after DM stripping on day 14. Following CEC scraping on day 3, cells that had migrated toward the central cornea underwent a transient fibrotic endothelial-mesenchymal transition (EMT) which was reversed back to an endothelial phenotype on day 14. However, in the corneas injured via DM stripping, most of the cells in the posterior fibrosis tissue did not originate from the corneal endothelium, and they maintained fibroblastic phenotype on day 14. We concluded that corneal endothelial wound healing in rabbits has different outcomes depending upon the presence or absence of Descemet’s membrane. Descemet’s membrane supports corneal endothelial cell regeneration in rabbits after endothelial injury.
format	article
author	Jingyao Chen Zhiyuan Li Liying Zhang Shangkun Ou Yanzi Wang Xin He Dulei Zou Changkai Jia Qianqian Hu Shu Yang Xian Li Juan Li Junqi Wang Huimin Sun Yongxiong Chen Ying-Ting Zhu Scheffer C. G. Tseng Zuguo Liu Wei Li
author_facet	Jingyao Chen Zhiyuan Li Liying Zhang Shangkun Ou Yanzi Wang Xin He Dulei Zou Changkai Jia Qianqian Hu Shu Yang Xian Li Juan Li Junqi Wang Huimin Sun Yongxiong Chen Ying-Ting Zhu Scheffer C. G. Tseng Zuguo Liu Wei Li
author_sort	Jingyao Chen
title	Descemet’s Membrane Supports Corneal Endothelial Cell Regeneration in Rabbits
title_short	Descemet’s Membrane Supports Corneal Endothelial Cell Regeneration in Rabbits
title_full	Descemet’s Membrane Supports Corneal Endothelial Cell Regeneration in Rabbits
title_fullStr	Descemet’s Membrane Supports Corneal Endothelial Cell Regeneration in Rabbits
title_full_unstemmed	Descemet’s Membrane Supports Corneal Endothelial Cell Regeneration in Rabbits
title_sort	descemet’s membrane supports corneal endothelial cell regeneration in rabbits
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/797519f7b29a44628ed3c0dc83b2fcb1
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Descemet’s Membrane Supports Corneal Endothelial Cell Regeneration in Rabbits

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