Broad scale proteomic analysis of heat-destabilised symbiosis in the hard coral Acropora millepora

Abstract Coral reefs across the globe are threatened by warming oceans. The last few years have seen the worst mass coral bleaching events recorded, with more than one quarter of all reefs irreversibly impacted. Considering the widespread devastation, we need to increase our efforts to understanding...

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Autores principales:	K. Petrou, B. L. Nunn, M. P. Padula, D. J. Miller, D. A. Nielsen
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/7985aae22b5741aa9bb5977c63ac9aca
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spelling	oai:doaj.org-article:7985aae22b5741aa9bb5977c63ac9aca2021-12-02T18:13:45ZBroad scale proteomic analysis of heat-destabilised symbiosis in the hard coral Acropora millepora10.1038/s41598-021-98548-x2045-2322https://doaj.org/article/7985aae22b5741aa9bb5977c63ac9aca2021-09-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-98548-xhttps://doaj.org/toc/2045-2322Abstract Coral reefs across the globe are threatened by warming oceans. The last few years have seen the worst mass coral bleaching events recorded, with more than one quarter of all reefs irreversibly impacted. Considering the widespread devastation, we need to increase our efforts to understanding the physiological and metabolic shifts underlying the breakdown of this important symbiotic ecosystem. Here, we investigated the proteome (PRIDE accession # PXD011668) of both host and symbionts of the reef-building coral Acropora millepora exposed to ambient (~ 28 °C) and elevated temperature (~ 32 °C for 2 days, following a five-day incremental increase) and explored associated biomolecular changes in the symbiont, with the aim of gaining new insights into the mechanisms underpinning the collapse of the coral symbiosis. We identified 1,230 unique proteins (774 host and 456 symbiont) in the control and thermally stressed corals, of which 107 significantly increased and 125 decreased in abundance under elevated temperature relative to the control. Proteins involved in oxidative stress and proteolysis constituted 29% of the host proteins that increased in abundance, with evidence of impairment to endoplasmic reticulum and cytoskeletal regulation proteins. In the symbiont, we detected a decrease in proteins responsible for photosynthesis and energy production (33% of proteins decreased in abundance), yet minimal signs of oxidative stress or proteolysis. Lipid stores increased > twofold despite reduction in photosynthesis, suggesting reduced translocation of carbon to the host. There were significant changes in proteins related to symbiotic state, including proteins linked to nitrogen metabolism in the host and the V-ATPase (-0.6 fold change) known to control symbiosome acidity. These results highlight key differences in host and symbiont proteomic adjustments under elevated temperature and identify two key proteins directly involved in bilateral nutrient exchange as potential indicators of symbiosis breakdown.K. PetrouB. L. NunnM. P. PadulaD. J. MillerD. A. NielsenNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-16 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q K. Petrou B. L. Nunn M. P. Padula D. J. Miller D. A. Nielsen Broad scale proteomic analysis of heat-destabilised symbiosis in the hard coral Acropora millepora
description	Abstract Coral reefs across the globe are threatened by warming oceans. The last few years have seen the worst mass coral bleaching events recorded, with more than one quarter of all reefs irreversibly impacted. Considering the widespread devastation, we need to increase our efforts to understanding the physiological and metabolic shifts underlying the breakdown of this important symbiotic ecosystem. Here, we investigated the proteome (PRIDE accession # PXD011668) of both host and symbionts of the reef-building coral Acropora millepora exposed to ambient (~ 28 °C) and elevated temperature (~ 32 °C for 2 days, following a five-day incremental increase) and explored associated biomolecular changes in the symbiont, with the aim of gaining new insights into the mechanisms underpinning the collapse of the coral symbiosis. We identified 1,230 unique proteins (774 host and 456 symbiont) in the control and thermally stressed corals, of which 107 significantly increased and 125 decreased in abundance under elevated temperature relative to the control. Proteins involved in oxidative stress and proteolysis constituted 29% of the host proteins that increased in abundance, with evidence of impairment to endoplasmic reticulum and cytoskeletal regulation proteins. In the symbiont, we detected a decrease in proteins responsible for photosynthesis and energy production (33% of proteins decreased in abundance), yet minimal signs of oxidative stress or proteolysis. Lipid stores increased > twofold despite reduction in photosynthesis, suggesting reduced translocation of carbon to the host. There were significant changes in proteins related to symbiotic state, including proteins linked to nitrogen metabolism in the host and the V-ATPase (-0.6 fold change) known to control symbiosome acidity. These results highlight key differences in host and symbiont proteomic adjustments under elevated temperature and identify two key proteins directly involved in bilateral nutrient exchange as potential indicators of symbiosis breakdown.
format	article
author	K. Petrou B. L. Nunn M. P. Padula D. J. Miller D. A. Nielsen
author_facet	K. Petrou B. L. Nunn M. P. Padula D. J. Miller D. A. Nielsen
author_sort	K. Petrou
title	Broad scale proteomic analysis of heat-destabilised symbiosis in the hard coral Acropora millepora
title_short	Broad scale proteomic analysis of heat-destabilised symbiosis in the hard coral Acropora millepora
title_full	Broad scale proteomic analysis of heat-destabilised symbiosis in the hard coral Acropora millepora
title_fullStr	Broad scale proteomic analysis of heat-destabilised symbiosis in the hard coral Acropora millepora
title_full_unstemmed	Broad scale proteomic analysis of heat-destabilised symbiosis in the hard coral Acropora millepora
title_sort	broad scale proteomic analysis of heat-destabilised symbiosis in the hard coral acropora millepora
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/7985aae22b5741aa9bb5977c63ac9aca
work_keys_str_mv	AT kpetrou broadscaleproteomicanalysisofheatdestabilisedsymbiosisinthehardcoralacroporamillepora AT blnunn broadscaleproteomicanalysisofheatdestabilisedsymbiosisinthehardcoralacroporamillepora AT mppadula broadscaleproteomicanalysisofheatdestabilisedsymbiosisinthehardcoralacroporamillepora AT djmiller broadscaleproteomicanalysisofheatdestabilisedsymbiosisinthehardcoralacroporamillepora AT danielsen broadscaleproteomicanalysisofheatdestabilisedsymbiosisinthehardcoralacroporamillepora
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Broad scale proteomic analysis of heat-destabilised symbiosis in the hard coral Acropora millepora

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