Detection of Low Density Lipoprotein—Comparison of Electrochemical Immuno- and Aptasensor

An elevated level of low density lipoprotein (LDL) can lead to the cardiovascular system-related diseases, such as atherosclerosis and others. Therefore, fast, simple, and accurate methods for LDL detection are very desirable. In this work, the parameters characterizing the electrochemical immuno-an...

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Auteurs principaux:	Daria Rudewicz-Kowalczyk, Iwona Grabowska
Format:	article
Langue:	EN
Publié:	MDPI AG 2021
Sujets:	low-density lipoprotein (LDL) aptasensor immunosensor electrochemical detection Chemical technology TP1-1185
Accès en ligne:	https://doaj.org/article/79901b11146744449a1b10d8e7eda2f6
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spelling	oai:doaj.org-article:79901b11146744449a1b10d8e7eda2f62021-11-25T18:58:52ZDetection of Low Density Lipoprotein—Comparison of Electrochemical Immuno- and Aptasensor10.3390/s212277331424-8220https://doaj.org/article/79901b11146744449a1b10d8e7eda2f62021-11-01T00:00:00Zhttps://www.mdpi.com/1424-8220/21/22/7733https://doaj.org/toc/1424-8220An elevated level of low density lipoprotein (LDL) can lead to the cardiovascular system-related diseases, such as atherosclerosis and others. Therefore, fast, simple, and accurate methods for LDL detection are very desirable. In this work, the parameters characterizing the electrochemical immuno-and aptasensor for detection of LDL have been compared for the first time. An immunosensor has been designed, for which the anti-apolipoprotein B-100 antibody was covalently attached to 4-aminothiophenol (4-ATP) on the surface of the gold electrode. In the case of an aptasensor, the gold electrode was modified in a mixture of ssDNA aptamer specific for LDL modified with –SH group and 6-mercaptohexanol. Square-wave voltammetry has been used for detection of LDL in PBS containing redox active marker, [Fe(CN)<sub>6</sub>]<sup>3−/4−</sup>. Our results show the linear dependence of [Fe(CN)<sub>6</sub>]<sup>3−/4−</sup> redox signal changes on LDL concentration for both biosensors, in the range from 0.01 ng/mL to 1.0 ng/mL. The limit of detection was 0.31 and 0.25 ng/mL, for immuno- and aptasensor, respectively. Whereas slightly better selectivity toward human serum albumin (HSA), high density lipoprotein (HDL), and malondialdehyde modified low density lipoprotein (MDA-LDL) has been observed for aptasensor. Moreover, the other components of human blood serum samples did not influence aptasensor sensitivity.Daria Rudewicz-KowalczykIwona GrabowskaMDPI AGarticlelow-density lipoprotein (LDL)aptasensorimmunosensorelectrochemical detectionChemical technologyTP1-1185ENSensors, Vol 21, Iss 7733, p 7733 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	low-density lipoprotein (LDL) aptasensor immunosensor electrochemical detection Chemical technology TP1-1185
spellingShingle	low-density lipoprotein (LDL) aptasensor immunosensor electrochemical detection Chemical technology TP1-1185 Daria Rudewicz-Kowalczyk Iwona Grabowska Detection of Low Density Lipoprotein—Comparison of Electrochemical Immuno- and Aptasensor
description	An elevated level of low density lipoprotein (LDL) can lead to the cardiovascular system-related diseases, such as atherosclerosis and others. Therefore, fast, simple, and accurate methods for LDL detection are very desirable. In this work, the parameters characterizing the electrochemical immuno-and aptasensor for detection of LDL have been compared for the first time. An immunosensor has been designed, for which the anti-apolipoprotein B-100 antibody was covalently attached to 4-aminothiophenol (4-ATP) on the surface of the gold electrode. In the case of an aptasensor, the gold electrode was modified in a mixture of ssDNA aptamer specific for LDL modified with –SH group and 6-mercaptohexanol. Square-wave voltammetry has been used for detection of LDL in PBS containing redox active marker, [Fe(CN)<sub>6</sub>]<sup>3−/4−</sup>. Our results show the linear dependence of [Fe(CN)<sub>6</sub>]<sup>3−/4−</sup> redox signal changes on LDL concentration for both biosensors, in the range from 0.01 ng/mL to 1.0 ng/mL. The limit of detection was 0.31 and 0.25 ng/mL, for immuno- and aptasensor, respectively. Whereas slightly better selectivity toward human serum albumin (HSA), high density lipoprotein (HDL), and malondialdehyde modified low density lipoprotein (MDA-LDL) has been observed for aptasensor. Moreover, the other components of human blood serum samples did not influence aptasensor sensitivity.
format	article
author	Daria Rudewicz-Kowalczyk Iwona Grabowska
author_facet	Daria Rudewicz-Kowalczyk Iwona Grabowska
author_sort	Daria Rudewicz-Kowalczyk
title	Detection of Low Density Lipoprotein—Comparison of Electrochemical Immuno- and Aptasensor
title_short	Detection of Low Density Lipoprotein—Comparison of Electrochemical Immuno- and Aptasensor
title_full	Detection of Low Density Lipoprotein—Comparison of Electrochemical Immuno- and Aptasensor
title_fullStr	Detection of Low Density Lipoprotein—Comparison of Electrochemical Immuno- and Aptasensor
title_full_unstemmed	Detection of Low Density Lipoprotein—Comparison of Electrochemical Immuno- and Aptasensor
title_sort	detection of low density lipoprotein—comparison of electrochemical immuno- and aptasensor
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/79901b11146744449a1b10d8e7eda2f6
work_keys_str_mv	AT dariarudewiczkowalczyk detectionoflowdensitylipoproteincomparisonofelectrochemicalimmunoandaptasensor AT iwonagrabowska detectionoflowdensitylipoproteincomparisonofelectrochemicalimmunoandaptasensor
_version_	1718410470691438592

Detection of Low Density Lipoprotein—Comparison of Electrochemical Immuno- and Aptasensor

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