Considerations for future environmental DNA accreditation and proficiency testing schemes

Abstract Environmental DNA (eDNA) monitoring has revolutionized the way biodiversity is surveyed and has been proposed as a reliable method to inform management decisions. Recognition of eDNA techniques as reliable tools to inform management and biosecurity require stringent standards to assess samp...

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Autores principales:	Alejandro Trujillo‐González, Cecilia Villacorta‐Rath, Nicole E. White, Elise M. Furlan, Mark Sykes, Geoff Grossel, Uday K. Divi, Dianne Gleeson
Formato:	article
Lenguaje:	EN
Publicado:	Wiley 2021
Materias:	accreditation International standard International Organization for Accreditation monitoring surveillance Environmental sciences GE1-350 Microbial ecology QR100-130
Acceso en línea:	https://doaj.org/article/799068d8969941fbbd1f1e870723348b
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spelling	oai:doaj.org-article:799068d8969941fbbd1f1e870723348b2021-11-23T16:15:24ZConsiderations for future environmental DNA accreditation and proficiency testing schemes2637-494310.1002/edn3.243https://doaj.org/article/799068d8969941fbbd1f1e870723348b2021-11-01T00:00:00Zhttps://doi.org/10.1002/edn3.243https://doaj.org/toc/2637-4943Abstract Environmental DNA (eDNA) monitoring has revolutionized the way biodiversity is surveyed and has been proposed as a reliable method to inform management decisions. Recognition of eDNA techniques as reliable tools to inform management and biosecurity require stringent standards to assess sample quality and reliability of results. Laboratories can have their workflows assessed and certified through accreditation and be involved in proficiency testing schemes provided to test the accuracy and precision of molecular methods. Currently, there is only one eDNA‐based proficiency testing scheme designed to test competency of laboratories in amplifying eDNA from the Great Crested Newt, Triturus cristatus (Laurenti, 1768) in water samples. This test, however, is a closed scheme currently run by invitation only to laboratories in the United Kingdom. Given the paucity of eDNA‐based proficiency schemes to ensure high‐quality services, this commentary discusses how future proficiency testing schemes could be designed to assess eDNA sample quality and reliability on detection results for environmental management and biosecurity applications. We discuss the use of tissue‐derived and synthetic oligonucleotides as reference materials, the need for proficiency testing schemes to assess the capacity of analytical facilities to determine sample quality as well as accurately detecting trace eDNA in blind samples and discuss the context in which fit for purpose eDNA testing schemes could be designed. To complement the future development of eDNA proficiency testing schemes, we provide firsthand accounts and lessons learned while developing the current Great Crested Newt eDNA proficiency scheme. Lastly, we highlight current limitations in standardizing rapidly improving eDNA‐based techniques and discuss disadvantages to accreditation and standardization from an Australian‐centered perspective as a means to promote an active debate on the topic of future eDNA accreditation and proficiency testing.Alejandro Trujillo‐GonzálezCecilia Villacorta‐RathNicole E. WhiteElise M. FurlanMark SykesGeoff GrosselUday K. DiviDianne GleesonWileyarticleaccreditationInternational standardInternational Organization for AccreditationmonitoringsurveillanceEnvironmental sciencesGE1-350Microbial ecologyQR100-130ENEnvironmental DNA, Vol 3, Iss 6, Pp 1049-1058 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	accreditation International standard International Organization for Accreditation monitoring surveillance Environmental sciences GE1-350 Microbial ecology QR100-130
spellingShingle	accreditation International standard International Organization for Accreditation monitoring surveillance Environmental sciences GE1-350 Microbial ecology QR100-130 Alejandro Trujillo‐González Cecilia Villacorta‐Rath Nicole E. White Elise M. Furlan Mark Sykes Geoff Grossel Uday K. Divi Dianne Gleeson Considerations for future environmental DNA accreditation and proficiency testing schemes
description	Abstract Environmental DNA (eDNA) monitoring has revolutionized the way biodiversity is surveyed and has been proposed as a reliable method to inform management decisions. Recognition of eDNA techniques as reliable tools to inform management and biosecurity require stringent standards to assess sample quality and reliability of results. Laboratories can have their workflows assessed and certified through accreditation and be involved in proficiency testing schemes provided to test the accuracy and precision of molecular methods. Currently, there is only one eDNA‐based proficiency testing scheme designed to test competency of laboratories in amplifying eDNA from the Great Crested Newt, Triturus cristatus (Laurenti, 1768) in water samples. This test, however, is a closed scheme currently run by invitation only to laboratories in the United Kingdom. Given the paucity of eDNA‐based proficiency schemes to ensure high‐quality services, this commentary discusses how future proficiency testing schemes could be designed to assess eDNA sample quality and reliability on detection results for environmental management and biosecurity applications. We discuss the use of tissue‐derived and synthetic oligonucleotides as reference materials, the need for proficiency testing schemes to assess the capacity of analytical facilities to determine sample quality as well as accurately detecting trace eDNA in blind samples and discuss the context in which fit for purpose eDNA testing schemes could be designed. To complement the future development of eDNA proficiency testing schemes, we provide firsthand accounts and lessons learned while developing the current Great Crested Newt eDNA proficiency scheme. Lastly, we highlight current limitations in standardizing rapidly improving eDNA‐based techniques and discuss disadvantages to accreditation and standardization from an Australian‐centered perspective as a means to promote an active debate on the topic of future eDNA accreditation and proficiency testing.
format	article
author	Alejandro Trujillo‐González Cecilia Villacorta‐Rath Nicole E. White Elise M. Furlan Mark Sykes Geoff Grossel Uday K. Divi Dianne Gleeson
author_facet	Alejandro Trujillo‐González Cecilia Villacorta‐Rath Nicole E. White Elise M. Furlan Mark Sykes Geoff Grossel Uday K. Divi Dianne Gleeson
author_sort	Alejandro Trujillo‐González
title	Considerations for future environmental DNA accreditation and proficiency testing schemes
title_short	Considerations for future environmental DNA accreditation and proficiency testing schemes
title_full	Considerations for future environmental DNA accreditation and proficiency testing schemes
title_fullStr	Considerations for future environmental DNA accreditation and proficiency testing schemes
title_full_unstemmed	Considerations for future environmental DNA accreditation and proficiency testing schemes
title_sort	considerations for future environmental dna accreditation and proficiency testing schemes
publisher	Wiley
publishDate	2021
url	https://doaj.org/article/799068d8969941fbbd1f1e870723348b
work_keys_str_mv	AT alejandrotrujillogonzalez considerationsforfutureenvironmentaldnaaccreditationandproficiencytestingschemes AT ceciliavillacortarath considerationsforfutureenvironmentaldnaaccreditationandproficiencytestingschemes AT nicoleewhite considerationsforfutureenvironmentaldnaaccreditationandproficiencytestingschemes AT elisemfurlan considerationsforfutureenvironmentaldnaaccreditationandproficiencytestingschemes AT marksykes considerationsforfutureenvironmentaldnaaccreditationandproficiencytestingschemes AT geoffgrossel considerationsforfutureenvironmentaldnaaccreditationandproficiencytestingschemes AT udaykdivi considerationsforfutureenvironmentaldnaaccreditationandproficiencytestingschemes AT diannegleeson considerationsforfutureenvironmentaldnaaccreditationandproficiencytestingschemes
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Considerations for future environmental DNA accreditation and proficiency testing schemes

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