Resistance to 16-Membered Macrolides, Tiamulin and Lincomycin in a Swine Isolate of Acholeplasma laidlawii

Acholeplasma (A.) laidlawii is an opportunistic pathogen with the ability to disseminate resistance determinants to antibiotics; however, its resistance to macrolides has been less studied. The aim of the present study was to characterize the...

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Autores principales:	María M. Tavío, Ana S. Ramírez, Carlos Poveda, Rubén S. Rosales, Cristina F. Malla, José B. Poveda
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	macrolide tiamulin lincomycin antimicrobial resistance <i>Acholeplasma laidlawii</i> Therapeutics. Pharmacology RM1-950
Acceso en línea:	https://doaj.org/article/79a01b9db65c4b0787c6a01b0c81ad21
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spelling	oai:doaj.org-article:79a01b9db65c4b0787c6a01b0c81ad212021-11-25T16:25:01ZResistance to 16-Membered Macrolides, Tiamulin and Lincomycin in a Swine Isolate of <i>Acholeplasma laidlawii</i>10.3390/antibiotics101114152079-6382https://doaj.org/article/79a01b9db65c4b0787c6a01b0c81ad212021-11-01T00:00:00Zhttps://www.mdpi.com/2079-6382/10/11/1415https://doaj.org/toc/2079-6382<i>Acholeplasma</i> (<i>A.</i>) <i>laidlawii</i> is an opportunistic pathogen with the ability to disseminate resistance determinants to antibiotics; however, its resistance to macrolides has been less studied. The aim of the present study was to characterize the mechanisms responsible for the resistance to macrolides, tiamulin and lincomycin found in a strain of <i>A. laidlawii</i> isolated from a pig with pneumonia. MICs of erythromycin, 15- and 16-membered macrolides, tiamulin and lincomycin were determined by microdilution method with and without reserpine, an inhibitor of ABC efflux pumps and regions of the genome were sequenced. Reserpine only decreased lincomycin MIC but it did not change the MICs of macrolides and tiamulin. The analysis of the DNA sequence of 23S rRNA showed nucleotide substitutions at eight different positions, although none of them were at positions previously related to macrolide resistance. Five mutations were found in the L22 protein, one of them at the stop codon. In addition, two mutations were found in the amino acid sequence of L4. The combination of multiple mutations in the ribosomal proteins L22 and L4 together with substitutions in 23S rRNA DNA sequence was associated with the resistance to macrolides, the pleuromutilin and lincomycin in the studied <i>A. laidlawii</i> strain.María M. TavíoAna S. RamírezCarlos PovedaRubén S. RosalesCristina F. MallaJosé B. PovedaMDPI AGarticlemacrolidetiamulinlincomycinantimicrobial resistance<i>Acholeplasma laidlawii</i>Therapeutics. PharmacologyRM1-950ENAntibiotics, Vol 10, Iss 1415, p 1415 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	macrolide tiamulin lincomycin antimicrobial resistance <i>Acholeplasma laidlawii</i> Therapeutics. Pharmacology RM1-950
spellingShingle	macrolide tiamulin lincomycin antimicrobial resistance <i>Acholeplasma laidlawii</i> Therapeutics. Pharmacology RM1-950 María M. Tavío Ana S. Ramírez Carlos Poveda Rubén S. Rosales Cristina F. Malla José B. Poveda Resistance to 16-Membered Macrolides, Tiamulin and Lincomycin in a Swine Isolate of <i>Acholeplasma laidlawii</i>
description	<i>Acholeplasma</i> (<i>A.</i>) <i>laidlawii</i> is an opportunistic pathogen with the ability to disseminate resistance determinants to antibiotics; however, its resistance to macrolides has been less studied. The aim of the present study was to characterize the mechanisms responsible for the resistance to macrolides, tiamulin and lincomycin found in a strain of <i>A. laidlawii</i> isolated from a pig with pneumonia. MICs of erythromycin, 15- and 16-membered macrolides, tiamulin and lincomycin were determined by microdilution method with and without reserpine, an inhibitor of ABC efflux pumps and regions of the genome were sequenced. Reserpine only decreased lincomycin MIC but it did not change the MICs of macrolides and tiamulin. The analysis of the DNA sequence of 23S rRNA showed nucleotide substitutions at eight different positions, although none of them were at positions previously related to macrolide resistance. Five mutations were found in the L22 protein, one of them at the stop codon. In addition, two mutations were found in the amino acid sequence of L4. The combination of multiple mutations in the ribosomal proteins L22 and L4 together with substitutions in 23S rRNA DNA sequence was associated with the resistance to macrolides, the pleuromutilin and lincomycin in the studied <i>A. laidlawii</i> strain.
format	article
author	María M. Tavío Ana S. Ramírez Carlos Poveda Rubén S. Rosales Cristina F. Malla José B. Poveda
author_facet	María M. Tavío Ana S. Ramírez Carlos Poveda Rubén S. Rosales Cristina F. Malla José B. Poveda
author_sort	María M. Tavío
title	Resistance to 16-Membered Macrolides, Tiamulin and Lincomycin in a Swine Isolate of <i>Acholeplasma laidlawii</i>
title_short	Resistance to 16-Membered Macrolides, Tiamulin and Lincomycin in a Swine Isolate of <i>Acholeplasma laidlawii</i>
title_full	Resistance to 16-Membered Macrolides, Tiamulin and Lincomycin in a Swine Isolate of <i>Acholeplasma laidlawii</i>
title_fullStr	Resistance to 16-Membered Macrolides, Tiamulin and Lincomycin in a Swine Isolate of <i>Acholeplasma laidlawii</i>
title_full_unstemmed	Resistance to 16-Membered Macrolides, Tiamulin and Lincomycin in a Swine Isolate of <i>Acholeplasma laidlawii</i>
title_sort	resistance to 16-membered macrolides, tiamulin and lincomycin in a swine isolate of <i>acholeplasma laidlawii</i>
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/79a01b9db65c4b0787c6a01b0c81ad21
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Resistance to 16-Membered Macrolides, Tiamulin and Lincomycin in a Swine Isolate of <i>Acholeplasma laidlawii</i>

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