Activation of multiple apoptotic pathways in human nasopharyngeal carcinoma cells by the prenylated isoflavone, osajin.

Osajin is a prenylated isoflavone showing antitumor activity in different tumor cell lines. The underlying mechanism of osajin-induced cancer cell death is not clearly understood. In the present study, the mechanisms of osajin-induced cell death of human nasopharyngeal carcinoma (NPC) cells were exp...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Tsung-Teng Huang, Fu-Guo Liu, Chia-Fong Wei, Chia-Chen Lu, Chang-Chieh Chen, Hung-Chi Lin, David M Ojcius, Hsin-Chih Lai
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2011
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/79a04fef5b66441e868d61268e6a3bdd
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:79a04fef5b66441e868d61268e6a3bdd
record_format	dspace
spelling	oai:doaj.org-article:79a04fef5b66441e868d61268e6a3bdd2021-11-18T06:55:55ZActivation of multiple apoptotic pathways in human nasopharyngeal carcinoma cells by the prenylated isoflavone, osajin.1932-620310.1371/journal.pone.0018308https://doaj.org/article/79a04fef5b66441e868d61268e6a3bdd2011-04-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/21532751/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203Osajin is a prenylated isoflavone showing antitumor activity in different tumor cell lines. The underlying mechanism of osajin-induced cancer cell death is not clearly understood. In the present study, the mechanisms of osajin-induced cell death of human nasopharyngeal carcinoma (NPC) cells were explored. Osajin was found to significantly induce apoptosis of NPC cells in a dose- and time-dependent manner. Multiple molecular effects were observed during osajin treatment including a significant loss of mitochondrial transmembrane potential, release of cytochrome c into the cytosol, enhanced expression of Fas ligand (FasL), suppression of glucose-regulated protein 78 kDa (GRP78), and activation of caspases-9, -8, -4 and -3. In addition, up-regulation of proapoptotic Bax protein and down-regulation of antiapoptotic Bcl-2 protein were also observed. Taken together, osajin induces apoptosis in human NPC cells through multiple apoptotic pathways, including the extrinsic death receptor pathway, and intrinsic pathways relying on mitochondria and endoplasmic reticulum stress. Thus, osajin could be developed as a new effective and chemopreventive compound for human NPC.Tsung-Teng HuangFu-Guo LiuChia-Fong WeiChia-Chen LuChang-Chieh ChenHung-Chi LinDavid M OjciusHsin-Chih LaiPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 6, Iss 4, p e18308 (2011)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Tsung-Teng Huang Fu-Guo Liu Chia-Fong Wei Chia-Chen Lu Chang-Chieh Chen Hung-Chi Lin David M Ojcius Hsin-Chih Lai Activation of multiple apoptotic pathways in human nasopharyngeal carcinoma cells by the prenylated isoflavone, osajin.
description	Osajin is a prenylated isoflavone showing antitumor activity in different tumor cell lines. The underlying mechanism of osajin-induced cancer cell death is not clearly understood. In the present study, the mechanisms of osajin-induced cell death of human nasopharyngeal carcinoma (NPC) cells were explored. Osajin was found to significantly induce apoptosis of NPC cells in a dose- and time-dependent manner. Multiple molecular effects were observed during osajin treatment including a significant loss of mitochondrial transmembrane potential, release of cytochrome c into the cytosol, enhanced expression of Fas ligand (FasL), suppression of glucose-regulated protein 78 kDa (GRP78), and activation of caspases-9, -8, -4 and -3. In addition, up-regulation of proapoptotic Bax protein and down-regulation of antiapoptotic Bcl-2 protein were also observed. Taken together, osajin induces apoptosis in human NPC cells through multiple apoptotic pathways, including the extrinsic death receptor pathway, and intrinsic pathways relying on mitochondria and endoplasmic reticulum stress. Thus, osajin could be developed as a new effective and chemopreventive compound for human NPC.
format	article
author	Tsung-Teng Huang Fu-Guo Liu Chia-Fong Wei Chia-Chen Lu Chang-Chieh Chen Hung-Chi Lin David M Ojcius Hsin-Chih Lai
author_facet	Tsung-Teng Huang Fu-Guo Liu Chia-Fong Wei Chia-Chen Lu Chang-Chieh Chen Hung-Chi Lin David M Ojcius Hsin-Chih Lai
author_sort	Tsung-Teng Huang
title	Activation of multiple apoptotic pathways in human nasopharyngeal carcinoma cells by the prenylated isoflavone, osajin.
title_short	Activation of multiple apoptotic pathways in human nasopharyngeal carcinoma cells by the prenylated isoflavone, osajin.
title_full	Activation of multiple apoptotic pathways in human nasopharyngeal carcinoma cells by the prenylated isoflavone, osajin.
title_fullStr	Activation of multiple apoptotic pathways in human nasopharyngeal carcinoma cells by the prenylated isoflavone, osajin.
title_full_unstemmed	Activation of multiple apoptotic pathways in human nasopharyngeal carcinoma cells by the prenylated isoflavone, osajin.
title_sort	activation of multiple apoptotic pathways in human nasopharyngeal carcinoma cells by the prenylated isoflavone, osajin.
publisher	Public Library of Science (PLoS)
publishDate	2011
url	https://doaj.org/article/79a04fef5b66441e868d61268e6a3bdd
work_keys_str_mv	AT tsungtenghuang activationofmultipleapoptoticpathwaysinhumannasopharyngealcarcinomacellsbytheprenylatedisoflavoneosajin AT fuguoliu activationofmultipleapoptoticpathwaysinhumannasopharyngealcarcinomacellsbytheprenylatedisoflavoneosajin AT chiafongwei activationofmultipleapoptoticpathwaysinhumannasopharyngealcarcinomacellsbytheprenylatedisoflavoneosajin AT chiachenlu activationofmultipleapoptoticpathwaysinhumannasopharyngealcarcinomacellsbytheprenylatedisoflavoneosajin AT changchiehchen activationofmultipleapoptoticpathwaysinhumannasopharyngealcarcinomacellsbytheprenylatedisoflavoneosajin AT hungchilin activationofmultipleapoptoticpathwaysinhumannasopharyngealcarcinomacellsbytheprenylatedisoflavoneosajin AT davidmojcius activationofmultipleapoptoticpathwaysinhumannasopharyngealcarcinomacellsbytheprenylatedisoflavoneosajin AT hsinchihlai activationofmultipleapoptoticpathwaysinhumannasopharyngealcarcinomacellsbytheprenylatedisoflavoneosajin
_version_	1718424174550056960

Activation of multiple apoptotic pathways in human nasopharyngeal carcinoma cells by the prenylated isoflavone, osajin.

Ejemplares similares