Aberrant Development of Enteric Glial Cells in the Colon of Hirschsprung's Disease

Objective: The aim of this study was to explore the development of enteric glial cells (EGCs) in different segments of Hirschsprung's disease (HSCR).Methods: Colonic specimens from 35 children with HSCR were selected to analyze the relative expression of glial fibrillary acidic protein and S100...

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Autores principales:	Tingting Zhou, Wei Liu, Xiaofang Yu, Zengcai Cao, Weijing Mu, Peimin Hou, Chuantao Ren, Aiwu Li
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	Hirschsprung's disease enteric glial cells enteric nervous system glial fibrillary acidic protein S100 calcium-binding protein B Pediatrics RJ1-570
Acceso en línea:	https://doaj.org/article/79ad465f798d4370b307edc38081e852
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spelling	oai:doaj.org-article:79ad465f798d4370b307edc38081e8522021-11-05T14:00:59ZAberrant Development of Enteric Glial Cells in the Colon of Hirschsprung's Disease2296-236010.3389/fped.2021.746274https://doaj.org/article/79ad465f798d4370b307edc38081e8522021-11-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fped.2021.746274/fullhttps://doaj.org/toc/2296-2360Objective: The aim of this study was to explore the development of enteric glial cells (EGCs) in different segments of Hirschsprung's disease (HSCR).Methods: Colonic specimens from 35 children with HSCR were selected to analyze the relative expression of glial fibrillary acidic protein and S100 calcium-binding protein B using Western blotting and real-time fluorescence quantitative PCR. Immunofluorescence and immunohistochemical staining were performed to determine the distribution of myenteric EGCs and neuronal cells in different segments of HSCR.Results: There was a trend of diminished protein and mRNA expression of glial fibrillary acidic protein and S100 calcium-binding protein B from the proximal, dilated, and transitional segments to the aganglionic segment (p < 0.05). Immunofluorescence and immunohistochemistry showed that the EGCs in the aganglionic, transitional, and dilated colonic muscles were morphologically abnormal, which was consistent with the dysplasia of myenteric neurons.Conclusion: Aberrant development of myenteric EGCs was observed in the colon of HSCR, which may affect the survival of enteric neurons.Tingting ZhouWei LiuXiaofang YuZengcai CaoWeijing MuPeimin HouChuantao RenChuantao RenAiwu LiFrontiers Media S.A.articleHirschsprung's diseaseenteric glial cellsenteric nervous systemglial fibrillary acidic proteinS100 calcium-binding protein BPediatricsRJ1-570ENFrontiers in Pediatrics, Vol 9 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Hirschsprung's disease enteric glial cells enteric nervous system glial fibrillary acidic protein S100 calcium-binding protein B Pediatrics RJ1-570
spellingShingle	Hirschsprung's disease enteric glial cells enteric nervous system glial fibrillary acidic protein S100 calcium-binding protein B Pediatrics RJ1-570 Tingting Zhou Wei Liu Xiaofang Yu Zengcai Cao Weijing Mu Peimin Hou Chuantao Ren Chuantao Ren Aiwu Li Aberrant Development of Enteric Glial Cells in the Colon of Hirschsprung's Disease
description	Objective: The aim of this study was to explore the development of enteric glial cells (EGCs) in different segments of Hirschsprung's disease (HSCR).Methods: Colonic specimens from 35 children with HSCR were selected to analyze the relative expression of glial fibrillary acidic protein and S100 calcium-binding protein B using Western blotting and real-time fluorescence quantitative PCR. Immunofluorescence and immunohistochemical staining were performed to determine the distribution of myenteric EGCs and neuronal cells in different segments of HSCR.Results: There was a trend of diminished protein and mRNA expression of glial fibrillary acidic protein and S100 calcium-binding protein B from the proximal, dilated, and transitional segments to the aganglionic segment (p < 0.05). Immunofluorescence and immunohistochemistry showed that the EGCs in the aganglionic, transitional, and dilated colonic muscles were morphologically abnormal, which was consistent with the dysplasia of myenteric neurons.Conclusion: Aberrant development of myenteric EGCs was observed in the colon of HSCR, which may affect the survival of enteric neurons.
format	article
author	Tingting Zhou Wei Liu Xiaofang Yu Zengcai Cao Weijing Mu Peimin Hou Chuantao Ren Chuantao Ren Aiwu Li
author_facet	Tingting Zhou Wei Liu Xiaofang Yu Zengcai Cao Weijing Mu Peimin Hou Chuantao Ren Chuantao Ren Aiwu Li
author_sort	Tingting Zhou
title	Aberrant Development of Enteric Glial Cells in the Colon of Hirschsprung's Disease
title_short	Aberrant Development of Enteric Glial Cells in the Colon of Hirschsprung's Disease
title_full	Aberrant Development of Enteric Glial Cells in the Colon of Hirschsprung's Disease
title_fullStr	Aberrant Development of Enteric Glial Cells in the Colon of Hirschsprung's Disease
title_full_unstemmed	Aberrant Development of Enteric Glial Cells in the Colon of Hirschsprung's Disease
title_sort	aberrant development of enteric glial cells in the colon of hirschsprung's disease
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/79ad465f798d4370b307edc38081e852
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Aberrant Development of Enteric Glial Cells in the Colon of Hirschsprung's Disease

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