Long non-coding RNA DUXAP8 elevates RCN2 expression and facilitates cell malignant behaviors and angiogenesis in cervical cancer via sponging miR-1297

Abstract Background Cervical cancer (CC) endangers women’s health in the world range. Accumulating studies have revealed the crucial regulatory role of long non-coding RNAs (lncRNAs) in multiple malignancies, including CC. Our study aimed to explore the role of lncRNA double homeobox A pseudogene 8...

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Autores principales:	Jihui Gu, Yi Liu, Ting Qi, Weiwei Qian, Dongdong Hu, Wen Feng
Formato:	article
Lenguaje:	EN
Publicado:	BMC 2021
Materias:	Cervical cancer DUXAP8 miR-1297 RCN2 Pathology RB1-214
Acceso en línea:	https://doaj.org/article/79b4a4faa7a54fde9b7af6ba208d4e12
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spelling	oai:doaj.org-article:79b4a4faa7a54fde9b7af6ba208d4e122021-11-14T12:37:44ZLong non-coding RNA DUXAP8 elevates RCN2 expression and facilitates cell malignant behaviors and angiogenesis in cervical cancer via sponging miR-129710.1186/s13000-021-01145-91746-1596https://doaj.org/article/79b4a4faa7a54fde9b7af6ba208d4e122021-11-01T00:00:00Zhttps://doi.org/10.1186/s13000-021-01145-9https://doaj.org/toc/1746-1596Abstract Background Cervical cancer (CC) endangers women’s health in the world range. Accumulating studies have revealed the crucial regulatory role of long non-coding RNAs (lncRNAs) in multiple malignancies, including CC. Our study aimed to explore the role of lncRNA double homeobox A pseudogene 8 (DUXAP8) in cervical carcinogenesis. Methods Gene expressions in CC were assessed by RT-qPCR. Function experiments and tube formation assays were performed to evaluate the role of DUXAP8 in CC cells. Subcellular fractionation and FISH assays were conducted to determine the subcellular location of DUXAP8. Luciferase reporter, RNA pull down and RIP assays were conducted to investigate the mechanism of DUXAP8. Results DUXAP8 was notably upregulated in CC cells. Downregulation of DUXAP8 repressed cell malignant behaviors and angiogenesis in CC. Mechanically, DUXAP8 boosted the expression of reticulocalbin-2 (RCN2) through relieving the binding of miR-1297 to RCN2 3’-UTR. Moreover, miR-1297 inhibition and RCN2 overexpression could counteract the inhibitory effects of DUXAP8 knockdown on the malignant phenotypes of CC cells. Besides, enhanced RCN2 expression restored the tumor growth in vivo that was inhibited by DUXAP8 repression. Conclusions DUXAP8 promotes malignant behaviors in CC cells via regulating miR-1297/RCN2 axis. Graphical AbstractJihui GuYi LiuTing QiWeiwei QianDongdong HuWen FengBMCarticleCervical cancerDUXAP8miR-1297RCN2PathologyRB1-214ENDiagnostic Pathology, Vol 16, Iss 1, Pp 1-12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Cervical cancer DUXAP8 miR-1297 RCN2 Pathology RB1-214
spellingShingle	Cervical cancer DUXAP8 miR-1297 RCN2 Pathology RB1-214 Jihui Gu Yi Liu Ting Qi Weiwei Qian Dongdong Hu Wen Feng Long non-coding RNA DUXAP8 elevates RCN2 expression and facilitates cell malignant behaviors and angiogenesis in cervical cancer via sponging miR-1297
description	Abstract Background Cervical cancer (CC) endangers women’s health in the world range. Accumulating studies have revealed the crucial regulatory role of long non-coding RNAs (lncRNAs) in multiple malignancies, including CC. Our study aimed to explore the role of lncRNA double homeobox A pseudogene 8 (DUXAP8) in cervical carcinogenesis. Methods Gene expressions in CC were assessed by RT-qPCR. Function experiments and tube formation assays were performed to evaluate the role of DUXAP8 in CC cells. Subcellular fractionation and FISH assays were conducted to determine the subcellular location of DUXAP8. Luciferase reporter, RNA pull down and RIP assays were conducted to investigate the mechanism of DUXAP8. Results DUXAP8 was notably upregulated in CC cells. Downregulation of DUXAP8 repressed cell malignant behaviors and angiogenesis in CC. Mechanically, DUXAP8 boosted the expression of reticulocalbin-2 (RCN2) through relieving the binding of miR-1297 to RCN2 3’-UTR. Moreover, miR-1297 inhibition and RCN2 overexpression could counteract the inhibitory effects of DUXAP8 knockdown on the malignant phenotypes of CC cells. Besides, enhanced RCN2 expression restored the tumor growth in vivo that was inhibited by DUXAP8 repression. Conclusions DUXAP8 promotes malignant behaviors in CC cells via regulating miR-1297/RCN2 axis. Graphical Abstract
format	article
author	Jihui Gu Yi Liu Ting Qi Weiwei Qian Dongdong Hu Wen Feng
author_facet	Jihui Gu Yi Liu Ting Qi Weiwei Qian Dongdong Hu Wen Feng
author_sort	Jihui Gu
title	Long non-coding RNA DUXAP8 elevates RCN2 expression and facilitates cell malignant behaviors and angiogenesis in cervical cancer via sponging miR-1297
title_short	Long non-coding RNA DUXAP8 elevates RCN2 expression and facilitates cell malignant behaviors and angiogenesis in cervical cancer via sponging miR-1297
title_full	Long non-coding RNA DUXAP8 elevates RCN2 expression and facilitates cell malignant behaviors and angiogenesis in cervical cancer via sponging miR-1297
title_fullStr	Long non-coding RNA DUXAP8 elevates RCN2 expression and facilitates cell malignant behaviors and angiogenesis in cervical cancer via sponging miR-1297
title_full_unstemmed	Long non-coding RNA DUXAP8 elevates RCN2 expression and facilitates cell malignant behaviors and angiogenesis in cervical cancer via sponging miR-1297
title_sort	long non-coding rna duxap8 elevates rcn2 expression and facilitates cell malignant behaviors and angiogenesis in cervical cancer via sponging mir-1297
publisher	BMC
publishDate	2021
url	https://doaj.org/article/79b4a4faa7a54fde9b7af6ba208d4e12
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Long non-coding RNA DUXAP8 elevates RCN2 expression and facilitates cell malignant behaviors and angiogenesis in cervical cancer via sponging miR-1297

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