Isolation of bacteria from Apis cerana hive, their antibacterial potency and cytotoxicity

Abstract. Yasmin Y, Fitri L, Fauziah, Wasliyah F. 2019. Isolation of bacteria from Apis cerana hive, their antibacterial potency and cytotoxicity. Biodiversitas 20: 2733-2738. This study aimed to identify bacteria from Apis cerana hive; to determine their antibacterial activity, and cytotoxic effect...

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Autores principales:	YEKKI YASMIN, Lenni Fitri, FAUZIAH, FITRI WASLIYAH
Formato:	article
Lenguaje:	EN
Publicado:	MBI & UNS Solo 2019
Materias:	antibacterial, apis cerana, bslt, hive bacteria, lc50 Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/79c0a64ce41248ee95b547e387cb4abf
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spelling	oai:doaj.org-article:79c0a64ce41248ee95b547e387cb4abf2021-11-21T21:54:25ZIsolation of bacteria from Apis cerana hive, their antibacterial potency and cytotoxicity1412-033X2085-472210.13057/biodiv/d200938https://doaj.org/article/79c0a64ce41248ee95b547e387cb4abf2019-08-01T00:00:00Zhttps://smujo.id/biodiv/article/view/4193https://doaj.org/toc/1412-033Xhttps://doaj.org/toc/2085-4722Abstract. Yasmin Y, Fitri L, Fauziah, Wasliyah F. 2019. Isolation of bacteria from Apis cerana hive, their antibacterial potency and cytotoxicity. Biodiversitas 20: 2733-2738. This study aimed to identify bacteria from Apis cerana hive; to determine their antibacterial activity, and cytotoxic effect of hive extract as well as bacterial extract. Identification of bacteria isolated from the hive was carried out based on morphological and biochemical characters. The antibacterial assay of beehive bacteria isolates was done by disk diffusion method against Staphylococcus aureus and Escherichia coli. Toxicity test of beehive extract and beehive bacterial extract were carried out using Brine Shrimp Lethality Test (BSLT) with six final concentrations (32 ppm, 64 ppm, 126 ppm, 250 ppm, 500 ppm, and 1000 ppm). The LC50 value was determined by probit analysis using SPSS. Bacterial isolation showed there were four bacterial isolates from A. cerana hive were the genus Bacillus (BSL1, and BSL3), the genus Micrococcus (BSL2) and the genus Neisseria (BSL4). The antibacterial assay showed that BSL4 isolate has the highest antibacterial activity against S. aureus with the diameter of the inhibitory zone was 32.6 mm. Toxicity test showed that A. cerana hive extract and bacterial ethanol extract from A. cerana hive had LC50 value of 67,744 ppm and 86.985 ppm respectively and categorized as toxic.YEKKI YASMINLenni FitriFAUZIAHFITRI WASLIYAHMBI & UNS Soloarticleantibacterial, apis cerana, bslt, hive bacteria, lc50Biology (General)QH301-705.5ENBiodiversitas, Vol 20, Iss 9 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	antibacterial, apis cerana, bslt, hive bacteria, lc50 Biology (General) QH301-705.5
spellingShingle	antibacterial, apis cerana, bslt, hive bacteria, lc50 Biology (General) QH301-705.5 YEKKI YASMIN Lenni Fitri FAUZIAH FITRI WASLIYAH Isolation of bacteria from Apis cerana hive, their antibacterial potency and cytotoxicity
description	Abstract. Yasmin Y, Fitri L, Fauziah, Wasliyah F. 2019. Isolation of bacteria from Apis cerana hive, their antibacterial potency and cytotoxicity. Biodiversitas 20: 2733-2738. This study aimed to identify bacteria from Apis cerana hive; to determine their antibacterial activity, and cytotoxic effect of hive extract as well as bacterial extract. Identification of bacteria isolated from the hive was carried out based on morphological and biochemical characters. The antibacterial assay of beehive bacteria isolates was done by disk diffusion method against Staphylococcus aureus and Escherichia coli. Toxicity test of beehive extract and beehive bacterial extract were carried out using Brine Shrimp Lethality Test (BSLT) with six final concentrations (32 ppm, 64 ppm, 126 ppm, 250 ppm, 500 ppm, and 1000 ppm). The LC50 value was determined by probit analysis using SPSS. Bacterial isolation showed there were four bacterial isolates from A. cerana hive were the genus Bacillus (BSL1, and BSL3), the genus Micrococcus (BSL2) and the genus Neisseria (BSL4). The antibacterial assay showed that BSL4 isolate has the highest antibacterial activity against S. aureus with the diameter of the inhibitory zone was 32.6 mm. Toxicity test showed that A. cerana hive extract and bacterial ethanol extract from A. cerana hive had LC50 value of 67,744 ppm and 86.985 ppm respectively and categorized as toxic.
format	article
author	YEKKI YASMIN Lenni Fitri FAUZIAH FITRI WASLIYAH
author_facet	YEKKI YASMIN Lenni Fitri FAUZIAH FITRI WASLIYAH
author_sort	YEKKI YASMIN
title	Isolation of bacteria from Apis cerana hive, their antibacterial potency and cytotoxicity
title_short	Isolation of bacteria from Apis cerana hive, their antibacterial potency and cytotoxicity
title_full	Isolation of bacteria from Apis cerana hive, their antibacterial potency and cytotoxicity
title_fullStr	Isolation of bacteria from Apis cerana hive, their antibacterial potency and cytotoxicity
title_full_unstemmed	Isolation of bacteria from Apis cerana hive, their antibacterial potency and cytotoxicity
title_sort	isolation of bacteria from apis cerana hive, their antibacterial potency and cytotoxicity
publisher	MBI & UNS Solo
publishDate	2019
url	https://doaj.org/article/79c0a64ce41248ee95b547e387cb4abf
work_keys_str_mv	AT yekkiyasmin isolationofbacteriafromapisceranahivetheirantibacterialpotencyandcytotoxicity AT lennifitri isolationofbacteriafromapisceranahivetheirantibacterialpotencyandcytotoxicity AT fauziah isolationofbacteriafromapisceranahivetheirantibacterialpotencyandcytotoxicity AT fitriwasliyah isolationofbacteriafromapisceranahivetheirantibacterialpotencyandcytotoxicity
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Isolation of bacteria from Apis cerana hive, their antibacterial potency and cytotoxicity

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