The Role of Phosphorus Limitation in Shaping Soil Bacterial Communities and Their Metabolic Capabilities

ABSTRACT Phosphorus (P) is an essential nutrient that is often in limited supply, with P availability constraining biomass production in many terrestrial ecosystems. Despite decades of work on plant responses to P deficiency and the importance of soil microbes to terrestrial ecosystem processes, how...

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Autores principales:	Angela M. Oliverio, Andrew Bissett, Krista McGuire, Kristin Saltonstall, Benjamin L. Turner, Noah Fierer
Formato:	article
Lenguaje:	EN
Publicado:	American Society for Microbiology 2020
Materias:	phosphorus limitation C-P lyase pathway organophosphonate degradation phosphate starvation soil microbiology Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/79c1a0f370fb49b997fe5c0d24fd6516
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spelling	oai:doaj.org-article:79c1a0f370fb49b997fe5c0d24fd65162021-11-15T16:19:09ZThe Role of Phosphorus Limitation in Shaping Soil Bacterial Communities and Their Metabolic Capabilities10.1128/mBio.01718-202150-7511https://doaj.org/article/79c1a0f370fb49b997fe5c0d24fd65162020-10-01T00:00:00Zhttps://journals.asm.org/doi/10.1128/mBio.01718-20https://doaj.org/toc/2150-7511ABSTRACT Phosphorus (P) is an essential nutrient that is often in limited supply, with P availability constraining biomass production in many terrestrial ecosystems. Despite decades of work on plant responses to P deficiency and the importance of soil microbes to terrestrial ecosystem processes, how soil microbes respond to, and cope with, P deficiencies remains poorly understood. We studied 583 soils from two independent sample sets that each span broad natural gradients in extractable soil P and collectively represent diverse biomes, including tropical forests, temperate grasslands, and arid shrublands. We paired marker gene and shotgun metagenomic analyses to determine how soil bacterial and archaeal communities respond to differences in soil P availability and to detect corresponding shifts in functional attributes. We identified microbial taxa that are consistently responsive to extractable soil P, with those taxa found in low P soils being more likely to have traits typical of oligotrophic life history strategies. Using environmental niche modeling of genes and gene pathways, we found an enriched abundance of key genes in low P soils linked to the carbon-phosphorus (C-P) lyase and phosphonotase degradation pathways, along with key components of the high-affinity phosphate-specific transporter (Pst) and phosphate regulon (Pho) systems. Taken together, these analyses suggest that catabolism of phosphonates is an important strategy used by bacteria to scavenge phosphate in P-limited soils. Surprisingly, these same pathways are important for bacterial growth in P-limited marine waters, highlighting the shared metabolic strategies used by both terrestrial and marine microbes to cope with P limitation.Angela M. OliverioAndrew BissettKrista McGuireKristin SaltonstallBenjamin L. TurnerNoah FiererAmerican Society for Microbiologyarticlephosphorus limitationC-P lyase pathwayorganophosphonate degradationphosphate starvationsoil microbiologyMicrobiologyQR1-502ENmBio, Vol 11, Iss 5 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	phosphorus limitation C-P lyase pathway organophosphonate degradation phosphate starvation soil microbiology Microbiology QR1-502
spellingShingle	phosphorus limitation C-P lyase pathway organophosphonate degradation phosphate starvation soil microbiology Microbiology QR1-502 Angela M. Oliverio Andrew Bissett Krista McGuire Kristin Saltonstall Benjamin L. Turner Noah Fierer The Role of Phosphorus Limitation in Shaping Soil Bacterial Communities and Their Metabolic Capabilities
description	ABSTRACT Phosphorus (P) is an essential nutrient that is often in limited supply, with P availability constraining biomass production in many terrestrial ecosystems. Despite decades of work on plant responses to P deficiency and the importance of soil microbes to terrestrial ecosystem processes, how soil microbes respond to, and cope with, P deficiencies remains poorly understood. We studied 583 soils from two independent sample sets that each span broad natural gradients in extractable soil P and collectively represent diverse biomes, including tropical forests, temperate grasslands, and arid shrublands. We paired marker gene and shotgun metagenomic analyses to determine how soil bacterial and archaeal communities respond to differences in soil P availability and to detect corresponding shifts in functional attributes. We identified microbial taxa that are consistently responsive to extractable soil P, with those taxa found in low P soils being more likely to have traits typical of oligotrophic life history strategies. Using environmental niche modeling of genes and gene pathways, we found an enriched abundance of key genes in low P soils linked to the carbon-phosphorus (C-P) lyase and phosphonotase degradation pathways, along with key components of the high-affinity phosphate-specific transporter (Pst) and phosphate regulon (Pho) systems. Taken together, these analyses suggest that catabolism of phosphonates is an important strategy used by bacteria to scavenge phosphate in P-limited soils. Surprisingly, these same pathways are important for bacterial growth in P-limited marine waters, highlighting the shared metabolic strategies used by both terrestrial and marine microbes to cope with P limitation.
format	article
author	Angela M. Oliverio Andrew Bissett Krista McGuire Kristin Saltonstall Benjamin L. Turner Noah Fierer
author_facet	Angela M. Oliverio Andrew Bissett Krista McGuire Kristin Saltonstall Benjamin L. Turner Noah Fierer
author_sort	Angela M. Oliverio
title	The Role of Phosphorus Limitation in Shaping Soil Bacterial Communities and Their Metabolic Capabilities
title_short	The Role of Phosphorus Limitation in Shaping Soil Bacterial Communities and Their Metabolic Capabilities
title_full	The Role of Phosphorus Limitation in Shaping Soil Bacterial Communities and Their Metabolic Capabilities
title_fullStr	The Role of Phosphorus Limitation in Shaping Soil Bacterial Communities and Their Metabolic Capabilities
title_full_unstemmed	The Role of Phosphorus Limitation in Shaping Soil Bacterial Communities and Their Metabolic Capabilities
title_sort	role of phosphorus limitation in shaping soil bacterial communities and their metabolic capabilities
publisher	American Society for Microbiology
publishDate	2020
url	https://doaj.org/article/79c1a0f370fb49b997fe5c0d24fd6516
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The Role of Phosphorus Limitation in Shaping Soil Bacterial Communities and Their Metabolic Capabilities

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