Indigenous endomycorrhizal fungi at salak (Salacca zalacca) plantations in Bali, Indonesia and their colonization of the roots

Abstract. Rai IN, Suada IK, Proborini M, Wiraatmaja IW, Semenov M, Krasnov G. 2019. Indigenous endomycorrhizal fungi at salak (Salacca zalacca) plantations in Bali, Indonesia and their colonization of the roots. Biodiversitas 20: 2410-2416. Cultivation of snake fruit, commonly known as salak usually...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	I Nyoman RAI, I Ketut Suada, Meitini Wahyuni Proborini, I Wayan Wiraatmaja, Mikhail SEMENOV, George Krasnov
Formato:	article
Lenguaje:	EN
Publicado:	MBI & UNS Solo 2019
Materias:	genetics, indigenous endomycorrhiza, infection rates, morphology, spores Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/79c22835aad24ade9896faefece6e062
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:79c22835aad24ade9896faefece6e062
record_format	dspace
spelling	oai:doaj.org-article:79c22835aad24ade9896faefece6e0622021-11-21T21:43:54ZIndigenous endomycorrhizal fungi at salak (Salacca zalacca) plantations in Bali, Indonesia and their colonization of the roots1412-033X2085-472210.13057/biodiv/d200840https://doaj.org/article/79c22835aad24ade9896faefece6e0622019-08-01T00:00:00Zhttps://smujo.id/biodiv/article/view/4001https://doaj.org/toc/1412-033Xhttps://doaj.org/toc/2085-4722Abstract. Rai IN, Suada IK, Proborini M, Wiraatmaja IW, Semenov M, Krasnov G. 2019. Indigenous endomycorrhizal fungi at salak (Salacca zalacca) plantations in Bali, Indonesia and their colonization of the roots. Biodiversitas 20: 2410-2416. Cultivation of snake fruit, commonly known as salak usually done organically on dry land with limited fertilizer in Bali. This research aimed to observe and to identify the indigenous endomycorrhizal fungi on salak roots. The exploration was carried out by collecting soil and root samples in salak producing areas in Bali, i.e. Bebandem and Selat of Karangasem Regency, Payangan of Gianyar, and Pupuan of Tabanan Regency. At each location, 9 random samples were taken, resulting in a total of 36 samples. Spore extraction was carried out using a wet filtration technique followed by centrifugation according to the method by Brunndrett et al. (2009). Morphological identification was carried out at the genus and species level using the Manual for Identification of Mycorrhiza Fungi for identifying Vesicular-Arbuscular-Mycorrhiza (VAM) fungi (Schenk and Perez, 1990), while molecular identification was carried out according to Tedersoo et al. (2014). The percentage of root infections was carried out using the coloring method with trypan blue. The results showed there were only two genera of endomycorrhizae (Glomus and Entrophospora) identified at the locations of study sites. The results also showed that samples from Bebandem and Selat regions had 3 Glomus species, Payangan had 3 Glomus species and 1 Entrophospora species, while in Pupuan had only 2 Glomus species. Identification results based on morphological characters showed that all species in the genus Glomus consisted of 3 species, namely Glomus sp-1, Glomus sp-2, and Glomus sp-3, while one species in the genus Entrophospora was Entrophospora sp. Genetic identification results based on the nucleotide arrangement showed that Glomus sp-1 concluded as Glomus cubence, Glomus sp-2 concluded as Glomus custos, and Glomus sp-3 concluded as Glomus indicum, while Entrophospora species concluded as Entrophospora_sp_SH197095.06FU. The average of root colonization/ infection was very high, reaching 93.33% in Bebandem and Selat, 95.00% in Pupuan, and 100% in Payangan. The very high root infection rates indicated that the indigenous endomycorrhiza found in these areas was very adaptive in salak plantation, so there is an opportunity to be developed as biofertilizers.I Nyoman RAII Ketut SuadaMeitini Wahyuni ProboriniI Wayan WiraatmajaMikhail SEMENOVGeorge KrasnovMBI & UNS Soloarticlegenetics, indigenous endomycorrhiza, infection rates, morphology, sporesBiology (General)QH301-705.5ENBiodiversitas, Vol 20, Iss 8 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	genetics, indigenous endomycorrhiza, infection rates, morphology, spores Biology (General) QH301-705.5
spellingShingle	genetics, indigenous endomycorrhiza, infection rates, morphology, spores Biology (General) QH301-705.5 I Nyoman RAI I Ketut Suada Meitini Wahyuni Proborini I Wayan Wiraatmaja Mikhail SEMENOV George Krasnov Indigenous endomycorrhizal fungi at salak (Salacca zalacca) plantations in Bali, Indonesia and their colonization of the roots
description	Abstract. Rai IN, Suada IK, Proborini M, Wiraatmaja IW, Semenov M, Krasnov G. 2019. Indigenous endomycorrhizal fungi at salak (Salacca zalacca) plantations in Bali, Indonesia and their colonization of the roots. Biodiversitas 20: 2410-2416. Cultivation of snake fruit, commonly known as salak usually done organically on dry land with limited fertilizer in Bali. This research aimed to observe and to identify the indigenous endomycorrhizal fungi on salak roots. The exploration was carried out by collecting soil and root samples in salak producing areas in Bali, i.e. Bebandem and Selat of Karangasem Regency, Payangan of Gianyar, and Pupuan of Tabanan Regency. At each location, 9 random samples were taken, resulting in a total of 36 samples. Spore extraction was carried out using a wet filtration technique followed by centrifugation according to the method by Brunndrett et al. (2009). Morphological identification was carried out at the genus and species level using the Manual for Identification of Mycorrhiza Fungi for identifying Vesicular-Arbuscular-Mycorrhiza (VAM) fungi (Schenk and Perez, 1990), while molecular identification was carried out according to Tedersoo et al. (2014). The percentage of root infections was carried out using the coloring method with trypan blue. The results showed there were only two genera of endomycorrhizae (Glomus and Entrophospora) identified at the locations of study sites. The results also showed that samples from Bebandem and Selat regions had 3 Glomus species, Payangan had 3 Glomus species and 1 Entrophospora species, while in Pupuan had only 2 Glomus species. Identification results based on morphological characters showed that all species in the genus Glomus consisted of 3 species, namely Glomus sp-1, Glomus sp-2, and Glomus sp-3, while one species in the genus Entrophospora was Entrophospora sp. Genetic identification results based on the nucleotide arrangement showed that Glomus sp-1 concluded as Glomus cubence, Glomus sp-2 concluded as Glomus custos, and Glomus sp-3 concluded as Glomus indicum, while Entrophospora species concluded as Entrophospora_sp_SH197095.06FU. The average of root colonization/ infection was very high, reaching 93.33% in Bebandem and Selat, 95.00% in Pupuan, and 100% in Payangan. The very high root infection rates indicated that the indigenous endomycorrhiza found in these areas was very adaptive in salak plantation, so there is an opportunity to be developed as biofertilizers.
format	article
author	I Nyoman RAI I Ketut Suada Meitini Wahyuni Proborini I Wayan Wiraatmaja Mikhail SEMENOV George Krasnov
author_facet	I Nyoman RAI I Ketut Suada Meitini Wahyuni Proborini I Wayan Wiraatmaja Mikhail SEMENOV George Krasnov
author_sort	I Nyoman RAI
title	Indigenous endomycorrhizal fungi at salak (Salacca zalacca) plantations in Bali, Indonesia and their colonization of the roots
title_short	Indigenous endomycorrhizal fungi at salak (Salacca zalacca) plantations in Bali, Indonesia and their colonization of the roots
title_full	Indigenous endomycorrhizal fungi at salak (Salacca zalacca) plantations in Bali, Indonesia and their colonization of the roots
title_fullStr	Indigenous endomycorrhizal fungi at salak (Salacca zalacca) plantations in Bali, Indonesia and their colonization of the roots
title_full_unstemmed	Indigenous endomycorrhizal fungi at salak (Salacca zalacca) plantations in Bali, Indonesia and their colonization of the roots
title_sort	indigenous endomycorrhizal fungi at salak (salacca zalacca) plantations in bali, indonesia and their colonization of the roots
publisher	MBI & UNS Solo
publishDate	2019
url	https://doaj.org/article/79c22835aad24ade9896faefece6e062
work_keys_str_mv	AT inyomanrai indigenousendomycorrhizalfungiatsalaksalaccazalaccaplantationsinbaliindonesiaandtheircolonizationoftheroots AT iketutsuada indigenousendomycorrhizalfungiatsalaksalaccazalaccaplantationsinbaliindonesiaandtheircolonizationoftheroots AT meitiniwahyuniproborini indigenousendomycorrhizalfungiatsalaksalaccazalaccaplantationsinbaliindonesiaandtheircolonizationoftheroots AT iwayanwiraatmaja indigenousendomycorrhizalfungiatsalaksalaccazalaccaplantationsinbaliindonesiaandtheircolonizationoftheroots AT mikhailsemenov indigenousendomycorrhizalfungiatsalaksalaccazalaccaplantationsinbaliindonesiaandtheircolonizationoftheroots AT georgekrasnov indigenousendomycorrhizalfungiatsalaksalaccazalaccaplantationsinbaliindonesiaandtheircolonizationoftheroots
_version_	1718418650147323904

Indigenous endomycorrhizal fungi at salak (Salacca zalacca) plantations in Bali, Indonesia and their colonization of the roots

Ejemplares similares