Rat macrophage C-type lectin is an activating receptor expressed by phagocytic cells.

Macrophage C-type lectin (MCL) is a membrane surface receptor encoded by the Antigen Presenting Lectin-like gene Complex (APLEC). We generated a mouse monoclonal antibody for the study of this receptor in the rat. We demonstrate that rat MCL is expressed on blood monocytes and neutrophils, as well a...

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Autores principales:	Ana Lobato-Pascual, Per Christian Saether, Maria K Dahle, Peter Gaustad, Erik Dissen, Sigbjørn Fossum, Michael R Daws
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/79c2ba5e3d4e45a992f0f7c29a41370e
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spelling	oai:doaj.org-article:79c2ba5e3d4e45a992f0f7c29a41370e2021-11-18T07:55:32ZRat macrophage C-type lectin is an activating receptor expressed by phagocytic cells.1932-620310.1371/journal.pone.0057406https://doaj.org/article/79c2ba5e3d4e45a992f0f7c29a41370e2013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23468983/?tool=EBIhttps://doaj.org/toc/1932-6203Macrophage C-type lectin (MCL) is a membrane surface receptor encoded by the Antigen Presenting Lectin-like gene Complex (APLEC). We generated a mouse monoclonal antibody for the study of this receptor in the rat. We demonstrate that rat MCL is expressed on blood monocytes and neutrophils, as well as on several tissue macrophage populations, including alveolar and peritoneal cavity macrophages. We also demonstrate MCL expression on a subset of resident spleen macrophages. Immunohistochemistry analysis of the spleen showed staining specifically in the marginal zone and red pulp. Exposure to pro-inflammatory mediators or to yeast cell wall extract (zymosan) increased surface MCL expression on peritoneal macrophages. We characterized a rat myeloid cell line, RMW, which expresses high levels of MCL. We found that MCL co-immunoprecipitated with the activating adaptor protein FcεRIγ in these cells. Moreover, beads coated with anti-MCL antibody increased phagocytosis in the RMW cells. Together, these observations indicate that rat MCL is a receptor that activates phagocytosis in myeloid cells under inflammatory conditions.Ana Lobato-PascualPer Christian SaetherMaria K DahlePeter GaustadErik DissenSigbjørn FossumMichael R DawsPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 2, p e57406 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Ana Lobato-Pascual Per Christian Saether Maria K Dahle Peter Gaustad Erik Dissen Sigbjørn Fossum Michael R Daws Rat macrophage C-type lectin is an activating receptor expressed by phagocytic cells.
description	Macrophage C-type lectin (MCL) is a membrane surface receptor encoded by the Antigen Presenting Lectin-like gene Complex (APLEC). We generated a mouse monoclonal antibody for the study of this receptor in the rat. We demonstrate that rat MCL is expressed on blood monocytes and neutrophils, as well as on several tissue macrophage populations, including alveolar and peritoneal cavity macrophages. We also demonstrate MCL expression on a subset of resident spleen macrophages. Immunohistochemistry analysis of the spleen showed staining specifically in the marginal zone and red pulp. Exposure to pro-inflammatory mediators or to yeast cell wall extract (zymosan) increased surface MCL expression on peritoneal macrophages. We characterized a rat myeloid cell line, RMW, which expresses high levels of MCL. We found that MCL co-immunoprecipitated with the activating adaptor protein FcεRIγ in these cells. Moreover, beads coated with anti-MCL antibody increased phagocytosis in the RMW cells. Together, these observations indicate that rat MCL is a receptor that activates phagocytosis in myeloid cells under inflammatory conditions.
format	article
author	Ana Lobato-Pascual Per Christian Saether Maria K Dahle Peter Gaustad Erik Dissen Sigbjørn Fossum Michael R Daws
author_facet	Ana Lobato-Pascual Per Christian Saether Maria K Dahle Peter Gaustad Erik Dissen Sigbjørn Fossum Michael R Daws
author_sort	Ana Lobato-Pascual
title	Rat macrophage C-type lectin is an activating receptor expressed by phagocytic cells.
title_short	Rat macrophage C-type lectin is an activating receptor expressed by phagocytic cells.
title_full	Rat macrophage C-type lectin is an activating receptor expressed by phagocytic cells.
title_fullStr	Rat macrophage C-type lectin is an activating receptor expressed by phagocytic cells.
title_full_unstemmed	Rat macrophage C-type lectin is an activating receptor expressed by phagocytic cells.
title_sort	rat macrophage c-type lectin is an activating receptor expressed by phagocytic cells.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/79c2ba5e3d4e45a992f0f7c29a41370e
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Rat macrophage C-type lectin is an activating receptor expressed by phagocytic cells.

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