Investigating the Transcriptome of Candida albicans in a Dual-Species Staphylococcus aureus Biofilm Model

Candida albicans is an opportunistic pathogen found throughout multiple body sites and is frequently co-isolated from infections of the respiratory tract and oral cavity with Staphylococcus aureus. Herein we present the first report of the effects that S. aureus elicits on the C. albicans transcript...

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Autores principales:	Bryn Short, Christopher Delaney, Emily McKloud, Jason L. Brown, Ryan Kean, Gary J. Litherland, Craig Williams, S. Lorraine Martin, William G. MacKay, Gordon Ramage
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	Candida Staphylococcus biofilm transcriptomics interkingdom interactions Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/79c41a5b8cea492a9446c40ef919642d
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spelling	oai:doaj.org-article:79c41a5b8cea492a9446c40ef919642d2021-11-30T14:09:34ZInvestigating the Transcriptome of Candida albicans in a Dual-Species Staphylococcus aureus Biofilm Model2235-298810.3389/fcimb.2021.791523https://doaj.org/article/79c41a5b8cea492a9446c40ef919642d2021-11-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fcimb.2021.791523/fullhttps://doaj.org/toc/2235-2988Candida albicans is an opportunistic pathogen found throughout multiple body sites and is frequently co-isolated from infections of the respiratory tract and oral cavity with Staphylococcus aureus. Herein we present the first report of the effects that S. aureus elicits on the C. albicans transcriptome. Dual-species biofilms containing S. aureus and C. albicans mutants defective in ALS3 or ECE1 were optimised and characterised, followed by transcriptional profiling of C. albicans by RNA-sequencing (RNA-seq). Altered phenotypes in C. albicans mutants revealed specific interaction profiles between fungus and bacteria. The major adhesion and virulence proteins Als3 and Ece1, respectively, were found to have substantial effects on the Candida transcriptome in early and mature biofilms. Despite this, deletion of ECE1 did not adversely affect biofilm formation or the ability of S. aureus to interact with C. albicans hyphae. Upregulated genes in dual-species biofilms corresponded to multiple gene ontology terms, including those attributed to virulence, biofilm formation and protein binding such as ACE2 and multiple heat-shock protein genes. This shows that S. aureus pushes C. albicans towards a more virulent genotype, helping us to understand the driving forces behind the increased severity of C. albicans-S. aureus infections.Bryn ShortBryn ShortBryn ShortChristopher DelaneyChristopher DelaneyEmily McKloudEmily McKloudJason L. BrownJason L. BrownRyan KeanRyan KeanGary J. LitherlandCraig WilliamsCraig WilliamsS. Lorraine MartinWilliam G. MacKayWilliam G. MacKayGordon RamageGordon RamageFrontiers Media S.A.articleCandidaStaphylococcusbiofilmtranscriptomicsinterkingdom interactionsMicrobiologyQR1-502ENFrontiers in Cellular and Infection Microbiology, Vol 11 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Candida Staphylococcus biofilm transcriptomics interkingdom interactions Microbiology QR1-502
spellingShingle	Candida Staphylococcus biofilm transcriptomics interkingdom interactions Microbiology QR1-502 Bryn Short Bryn Short Bryn Short Christopher Delaney Christopher Delaney Emily McKloud Emily McKloud Jason L. Brown Jason L. Brown Ryan Kean Ryan Kean Gary J. Litherland Craig Williams Craig Williams S. Lorraine Martin William G. MacKay William G. MacKay Gordon Ramage Gordon Ramage Investigating the Transcriptome of Candida albicans in a Dual-Species Staphylococcus aureus Biofilm Model
description	Candida albicans is an opportunistic pathogen found throughout multiple body sites and is frequently co-isolated from infections of the respiratory tract and oral cavity with Staphylococcus aureus. Herein we present the first report of the effects that S. aureus elicits on the C. albicans transcriptome. Dual-species biofilms containing S. aureus and C. albicans mutants defective in ALS3 or ECE1 were optimised and characterised, followed by transcriptional profiling of C. albicans by RNA-sequencing (RNA-seq). Altered phenotypes in C. albicans mutants revealed specific interaction profiles between fungus and bacteria. The major adhesion and virulence proteins Als3 and Ece1, respectively, were found to have substantial effects on the Candida transcriptome in early and mature biofilms. Despite this, deletion of ECE1 did not adversely affect biofilm formation or the ability of S. aureus to interact with C. albicans hyphae. Upregulated genes in dual-species biofilms corresponded to multiple gene ontology terms, including those attributed to virulence, biofilm formation and protein binding such as ACE2 and multiple heat-shock protein genes. This shows that S. aureus pushes C. albicans towards a more virulent genotype, helping us to understand the driving forces behind the increased severity of C. albicans-S. aureus infections.
format	article
author	Bryn Short Bryn Short Bryn Short Christopher Delaney Christopher Delaney Emily McKloud Emily McKloud Jason L. Brown Jason L. Brown Ryan Kean Ryan Kean Gary J. Litherland Craig Williams Craig Williams S. Lorraine Martin William G. MacKay William G. MacKay Gordon Ramage Gordon Ramage
author_facet	Bryn Short Bryn Short Bryn Short Christopher Delaney Christopher Delaney Emily McKloud Emily McKloud Jason L. Brown Jason L. Brown Ryan Kean Ryan Kean Gary J. Litherland Craig Williams Craig Williams S. Lorraine Martin William G. MacKay William G. MacKay Gordon Ramage Gordon Ramage
author_sort	Bryn Short
title	Investigating the Transcriptome of Candida albicans in a Dual-Species Staphylococcus aureus Biofilm Model
title_short	Investigating the Transcriptome of Candida albicans in a Dual-Species Staphylococcus aureus Biofilm Model
title_full	Investigating the Transcriptome of Candida albicans in a Dual-Species Staphylococcus aureus Biofilm Model
title_fullStr	Investigating the Transcriptome of Candida albicans in a Dual-Species Staphylococcus aureus Biofilm Model
title_full_unstemmed	Investigating the Transcriptome of Candida albicans in a Dual-Species Staphylococcus aureus Biofilm Model
title_sort	investigating the transcriptome of candida albicans in a dual-species staphylococcus aureus biofilm model
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/79c41a5b8cea492a9446c40ef919642d
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Investigating the Transcriptome of Candida albicans in a Dual-Species Staphylococcus aureus Biofilm Model

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