Engineering an efficient and bright split Corynactis californica green fluorescent protein

Abstract Split green fluorescent protein (GFP) has been used in a panoply of cellular biology applications to study protein translocation, monitor protein solubility and aggregation, detect protein–protein interactions, enhance protein crystallization, and even map neuron contacts. Recent work shows...

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Autores principales:	Hau B. Nguyen, Thomas C. Terwilliger, Geoffrey S. Waldo
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/79c928c86b214c6d82d1df592da8ced9
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spelling	oai:doaj.org-article:79c928c86b214c6d82d1df592da8ced92021-12-02T18:02:23ZEngineering an efficient and bright split Corynactis californica green fluorescent protein10.1038/s41598-021-98149-82045-2322https://doaj.org/article/79c928c86b214c6d82d1df592da8ced92021-09-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-98149-8https://doaj.org/toc/2045-2322Abstract Split green fluorescent protein (GFP) has been used in a panoply of cellular biology applications to study protein translocation, monitor protein solubility and aggregation, detect protein–protein interactions, enhance protein crystallization, and even map neuron contacts. Recent work shows the utility of split fluorescent proteins for large scale labeling of proteins in cells using CRISPR, but sets of efficient split fluorescent proteins that do not cross-react are needed for multiplexing experiments. We present a new monomeric split green fluorescent protein (ccGFP) engineered from a tetrameric GFP found in Corynactis californica, a bright red colonial anthozoan similar to sea anemones and scleractinian stony corals. Split ccGFP from C. californica complements up to threefold faster compared to the original Aequorea victoria split GFP and enable multiplexed labeling with existing A. victoria split YFP and CFP.Hau B. NguyenThomas C. TerwilligerGeoffrey S. WaldoNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-15 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Hau B. Nguyen Thomas C. Terwilliger Geoffrey S. Waldo Engineering an efficient and bright split Corynactis californica green fluorescent protein
description	Abstract Split green fluorescent protein (GFP) has been used in a panoply of cellular biology applications to study protein translocation, monitor protein solubility and aggregation, detect protein–protein interactions, enhance protein crystallization, and even map neuron contacts. Recent work shows the utility of split fluorescent proteins for large scale labeling of proteins in cells using CRISPR, but sets of efficient split fluorescent proteins that do not cross-react are needed for multiplexing experiments. We present a new monomeric split green fluorescent protein (ccGFP) engineered from a tetrameric GFP found in Corynactis californica, a bright red colonial anthozoan similar to sea anemones and scleractinian stony corals. Split ccGFP from C. californica complements up to threefold faster compared to the original Aequorea victoria split GFP and enable multiplexed labeling with existing A. victoria split YFP and CFP.
format	article
author	Hau B. Nguyen Thomas C. Terwilliger Geoffrey S. Waldo
author_facet	Hau B. Nguyen Thomas C. Terwilliger Geoffrey S. Waldo
author_sort	Hau B. Nguyen
title	Engineering an efficient and bright split Corynactis californica green fluorescent protein
title_short	Engineering an efficient and bright split Corynactis californica green fluorescent protein
title_full	Engineering an efficient and bright split Corynactis californica green fluorescent protein
title_fullStr	Engineering an efficient and bright split Corynactis californica green fluorescent protein
title_full_unstemmed	Engineering an efficient and bright split Corynactis californica green fluorescent protein
title_sort	engineering an efficient and bright split corynactis californica green fluorescent protein
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/79c928c86b214c6d82d1df592da8ced9
work_keys_str_mv	AT haubnguyen engineeringanefficientandbrightsplitcorynactiscalifornicagreenfluorescentprotein AT thomascterwilliger engineeringanefficientandbrightsplitcorynactiscalifornicagreenfluorescentprotein AT geoffreyswaldo engineeringanefficientandbrightsplitcorynactiscalifornicagreenfluorescentprotein
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Engineering an efficient and bright split Corynactis californica green fluorescent protein

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