The circular RNA hsa_circ_000780 as a potential molecular diagnostic target for gastric cancer

Abstract Background The present study aimed to identify a specific circular RNA (circRNA) for early diagnosis of gastric cancer (GC). Methods Totally 82 patients with GC, 30 with chronic nonatrophic gastritis and 30 with chronic atrophic gastritis were included in this study. Four of the 82 GC patie...

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Autores principales:	Jian Song, Shuyong Yu, Dunjing Zhong, Weizhong Yang, Zhen Jia, Guihong Yuan, Ping Li, Ronglin Zhang, Yini Li, Guobing Zhong, Zhaowei Chen
Formato:	article
Lenguaje:	EN
Publicado:	BMC 2021
Materias:	Circular RNA Gastric cancer hsa_circ_000780 Internal medicine RC31-1245 Genetics QH426-470
Acceso en línea:	https://doaj.org/article/79d28168eac44c4c9724d7ffb832557b
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spelling	oai:doaj.org-article:79d28168eac44c4c9724d7ffb832557b2021-11-28T12:05:59ZThe circular RNA hsa_circ_000780 as a potential molecular diagnostic target for gastric cancer10.1186/s12920-021-01096-61755-8794https://doaj.org/article/79d28168eac44c4c9724d7ffb832557b2021-11-01T00:00:00Zhttps://doi.org/10.1186/s12920-021-01096-6https://doaj.org/toc/1755-8794Abstract Background The present study aimed to identify a specific circular RNA (circRNA) for early diagnosis of gastric cancer (GC). Methods Totally 82 patients with GC, 30 with chronic nonatrophic gastritis and 30 with chronic atrophic gastritis were included in this study. Four of the 82 GC patients were selected for screening. Total RNA from malignant and adjacent tissue samples was extracted, and circRNAs in four patients were screened. According to the screening results, the eight most upregulated and downregulated circRNAs with a statistically significant association with GC were identified by real-time fluorescent quantitative polymerase chain reaction (PCR). Then, the most regulated circRNA was selected for further sensitivity and specificity assessments. CircRNA expression was examined by quantitative reverse transcriptase PCR in 78 GC (21 and 57 early and advanced GC, respectively) and adjacent tissue samples, as well as in gastric fluid samples from 30 patients with chronic nonatrophic gastritis, 30 with chronic atrophic gastritis, and 78 GC. Results A total of 445 circRNAs, including 69 upregulated and 376 downregulated circRNAs, showed significantly altered expression in GC tissue samples. Hsa_circ_000780 was significantly downregulated in 80.77% of GC tissue samples, with levels in GC tissue samples correlating with tumor size, tumor stage, T stage, venous invasion, carcinoembryonic antigen amounts, and carbohydrate antigen 19–9 levels. Strikingly, this circRNA was found in the gastric fluid of patients with early and advanced GC. Conclusions The present study uncovered a new circRNA expression profile in human GC, with hsa_circ_000780 significantly downregulated in GC tissue and gastric fluid specimens. These findings indicate that hsa_circ_000780 should be considered a novel biomarker for early GC screening.Jian SongShuyong YuDunjing ZhongWeizhong YangZhen JiaGuihong YuanPing LiRonglin ZhangYini LiGuobing ZhongZhaowei ChenBMCarticleCircular RNAGastric cancerhsa_circ_000780Internal medicineRC31-1245GeneticsQH426-470ENBMC Medical Genomics, Vol 14, Iss 1, Pp 1-10 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Circular RNA Gastric cancer hsa_circ_000780 Internal medicine RC31-1245 Genetics QH426-470
spellingShingle	Circular RNA Gastric cancer hsa_circ_000780 Internal medicine RC31-1245 Genetics QH426-470 Jian Song Shuyong Yu Dunjing Zhong Weizhong Yang Zhen Jia Guihong Yuan Ping Li Ronglin Zhang Yini Li Guobing Zhong Zhaowei Chen The circular RNA hsa_circ_000780 as a potential molecular diagnostic target for gastric cancer
description	Abstract Background The present study aimed to identify a specific circular RNA (circRNA) for early diagnosis of gastric cancer (GC). Methods Totally 82 patients with GC, 30 with chronic nonatrophic gastritis and 30 with chronic atrophic gastritis were included in this study. Four of the 82 GC patients were selected for screening. Total RNA from malignant and adjacent tissue samples was extracted, and circRNAs in four patients were screened. According to the screening results, the eight most upregulated and downregulated circRNAs with a statistically significant association with GC were identified by real-time fluorescent quantitative polymerase chain reaction (PCR). Then, the most regulated circRNA was selected for further sensitivity and specificity assessments. CircRNA expression was examined by quantitative reverse transcriptase PCR in 78 GC (21 and 57 early and advanced GC, respectively) and adjacent tissue samples, as well as in gastric fluid samples from 30 patients with chronic nonatrophic gastritis, 30 with chronic atrophic gastritis, and 78 GC. Results A total of 445 circRNAs, including 69 upregulated and 376 downregulated circRNAs, showed significantly altered expression in GC tissue samples. Hsa_circ_000780 was significantly downregulated in 80.77% of GC tissue samples, with levels in GC tissue samples correlating with tumor size, tumor stage, T stage, venous invasion, carcinoembryonic antigen amounts, and carbohydrate antigen 19–9 levels. Strikingly, this circRNA was found in the gastric fluid of patients with early and advanced GC. Conclusions The present study uncovered a new circRNA expression profile in human GC, with hsa_circ_000780 significantly downregulated in GC tissue and gastric fluid specimens. These findings indicate that hsa_circ_000780 should be considered a novel biomarker for early GC screening.
format	article
author	Jian Song Shuyong Yu Dunjing Zhong Weizhong Yang Zhen Jia Guihong Yuan Ping Li Ronglin Zhang Yini Li Guobing Zhong Zhaowei Chen
author_facet	Jian Song Shuyong Yu Dunjing Zhong Weizhong Yang Zhen Jia Guihong Yuan Ping Li Ronglin Zhang Yini Li Guobing Zhong Zhaowei Chen
author_sort	Jian Song
title	The circular RNA hsa_circ_000780 as a potential molecular diagnostic target for gastric cancer
title_short	The circular RNA hsa_circ_000780 as a potential molecular diagnostic target for gastric cancer
title_full	The circular RNA hsa_circ_000780 as a potential molecular diagnostic target for gastric cancer
title_fullStr	The circular RNA hsa_circ_000780 as a potential molecular diagnostic target for gastric cancer
title_full_unstemmed	The circular RNA hsa_circ_000780 as a potential molecular diagnostic target for gastric cancer
title_sort	circular rna hsa_circ_000780 as a potential molecular diagnostic target for gastric cancer
publisher	BMC
publishDate	2021
url	https://doaj.org/article/79d28168eac44c4c9724d7ffb832557b
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The circular RNA hsa_circ_000780 as a potential molecular diagnostic target for gastric cancer

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