Susceptibility to <italic toggle="yes">Campylobacter</italic> Infection Is Associated with the Species Composition of the Human Fecal Microbiota

ABSTRACT The gut microbiota is essential for human health, but very little is known about how the composition of this ecosystem can influence and respond to bacterial infections. Here we address this by prospectively studying the gut microbiota composition before, during, and after natural Campyloba...

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Autores principales:	Johan Dicksved, Patrik Ellström, Lars Engstrand, Hilpi Rautelin
Formato:	article
Lenguaje:	EN
Publicado:	American Society for Microbiology 2014
Materias:	Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/79e01add8d2c4d66b15c4c7d3c9a3957
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spelling	oai:doaj.org-article:79e01add8d2c4d66b15c4c7d3c9a39572021-11-15T15:45:54ZSusceptibility to <italic toggle="yes">Campylobacter</italic> Infection Is Associated with the Species Composition of the Human Fecal Microbiota10.1128/mBio.01212-142150-7511https://doaj.org/article/79e01add8d2c4d66b15c4c7d3c9a39572014-10-01T00:00:00Zhttps://journals.asm.org/doi/10.1128/mBio.01212-14https://doaj.org/toc/2150-7511ABSTRACT The gut microbiota is essential for human health, but very little is known about how the composition of this ecosystem can influence and respond to bacterial infections. Here we address this by prospectively studying the gut microbiota composition before, during, and after natural Campylobacter infection in exposed poultry abattoir workers. The gut microbiota composition was analyzed with 16S amplicon sequencing of fecal samples from poultry abattoir workers during the peak season of Campylobacter infection in Sweden. The gut microbiota compositions were compared between individuals who became culture positive for Campylobacter and those who remained negative. Individuals who became Campylobacter positive had a significantly higher abundance of Bacteroides (P = 0.007) and Escherichia (P = 0.002) species than those who remained culture negative. Furthermore, this group had a significantly higher abundance of Phascolarctobacterium (P = 0.017) and Streptococcus (P = 0.034) sequences than the Campylobacter-negative group, which had an overrepresentation of Clostridiales (P = 0.017), unclassified Lachnospiraceae (P = 0.008), and Anaerovorax (P = 0.015) sequences. Intraindividual comparisons of the fecal microbiota compositions yielded small differences over time in Campylobacter-negative participants, but significant long-term changes were found in the Campylobacter-positive group (P < 0.005). The results suggest that the abundance of specific genera in the microbiota reduces resistance to Campylobacter colonization in humans and that Campylobacter infection can have long-term effects on the composition of the human fecal microbiota. IMPORTANCE Studies using mouse models have made important contributions to our understanding of the role of the gut microbiota in resistance to bacterial enteropathogen colonization. The relative abundances of Escherichia coli and Bacteroides species have been pointed out as important determinants of susceptibility to Gram-negative pathogens in general and Campylobacter infection in particular. In this study, we assessed the role of the human gut microbiota in resistance to Campylobacter colonization by studying abattoir workers that are heavily exposed to these bacteria. Individuals with a certain composition of the gut microbiota became culture positive for Campylobacter. As their microbiotas were characterized by high abundances of Bacteroides spp. and E. coli, well in line with the findings with mouse models, these bacterial species likely play an important role in colonization resistance also in humans.Johan DicksvedPatrik EllströmLars EngstrandHilpi RautelinAmerican Society for MicrobiologyarticleMicrobiologyQR1-502ENmBio, Vol 5, Iss 5 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Microbiology QR1-502
spellingShingle	Microbiology QR1-502 Johan Dicksved Patrik Ellström Lars Engstrand Hilpi Rautelin Susceptibility to <italic toggle="yes">Campylobacter</italic> Infection Is Associated with the Species Composition of the Human Fecal Microbiota
description	ABSTRACT The gut microbiota is essential for human health, but very little is known about how the composition of this ecosystem can influence and respond to bacterial infections. Here we address this by prospectively studying the gut microbiota composition before, during, and after natural Campylobacter infection in exposed poultry abattoir workers. The gut microbiota composition was analyzed with 16S amplicon sequencing of fecal samples from poultry abattoir workers during the peak season of Campylobacter infection in Sweden. The gut microbiota compositions were compared between individuals who became culture positive for Campylobacter and those who remained negative. Individuals who became Campylobacter positive had a significantly higher abundance of Bacteroides (P = 0.007) and Escherichia (P = 0.002) species than those who remained culture negative. Furthermore, this group had a significantly higher abundance of Phascolarctobacterium (P = 0.017) and Streptococcus (P = 0.034) sequences than the Campylobacter-negative group, which had an overrepresentation of Clostridiales (P = 0.017), unclassified Lachnospiraceae (P = 0.008), and Anaerovorax (P = 0.015) sequences. Intraindividual comparisons of the fecal microbiota compositions yielded small differences over time in Campylobacter-negative participants, but significant long-term changes were found in the Campylobacter-positive group (P < 0.005). The results suggest that the abundance of specific genera in the microbiota reduces resistance to Campylobacter colonization in humans and that Campylobacter infection can have long-term effects on the composition of the human fecal microbiota. IMPORTANCE Studies using mouse models have made important contributions to our understanding of the role of the gut microbiota in resistance to bacterial enteropathogen colonization. The relative abundances of Escherichia coli and Bacteroides species have been pointed out as important determinants of susceptibility to Gram-negative pathogens in general and Campylobacter infection in particular. In this study, we assessed the role of the human gut microbiota in resistance to Campylobacter colonization by studying abattoir workers that are heavily exposed to these bacteria. Individuals with a certain composition of the gut microbiota became culture positive for Campylobacter. As their microbiotas were characterized by high abundances of Bacteroides spp. and E. coli, well in line with the findings with mouse models, these bacterial species likely play an important role in colonization resistance also in humans.
format	article
author	Johan Dicksved Patrik Ellström Lars Engstrand Hilpi Rautelin
author_facet	Johan Dicksved Patrik Ellström Lars Engstrand Hilpi Rautelin
author_sort	Johan Dicksved
title	Susceptibility to <italic toggle="yes">Campylobacter</italic> Infection Is Associated with the Species Composition of the Human Fecal Microbiota
title_short	Susceptibility to <italic toggle="yes">Campylobacter</italic> Infection Is Associated with the Species Composition of the Human Fecal Microbiota
title_full	Susceptibility to <italic toggle="yes">Campylobacter</italic> Infection Is Associated with the Species Composition of the Human Fecal Microbiota
title_fullStr	Susceptibility to <italic toggle="yes">Campylobacter</italic> Infection Is Associated with the Species Composition of the Human Fecal Microbiota
title_full_unstemmed	Susceptibility to <italic toggle="yes">Campylobacter</italic> Infection Is Associated with the Species Composition of the Human Fecal Microbiota
title_sort	susceptibility to <italic toggle="yes">campylobacter</italic> infection is associated with the species composition of the human fecal microbiota
publisher	American Society for Microbiology
publishDate	2014
url	https://doaj.org/article/79e01add8d2c4d66b15c4c7d3c9a3957
work_keys_str_mv	AT johandicksved susceptibilitytoitalictoggleyescampylobacteritalicinfectionisassociatedwiththespeciescompositionofthehumanfecalmicrobiota AT patrikellstrom susceptibilitytoitalictoggleyescampylobacteritalicinfectionisassociatedwiththespeciescompositionofthehumanfecalmicrobiota AT larsengstrand susceptibilitytoitalictoggleyescampylobacteritalicinfectionisassociatedwiththespeciescompositionofthehumanfecalmicrobiota AT hilpirautelin susceptibilitytoitalictoggleyescampylobacteritalicinfectionisassociatedwiththespeciescompositionofthehumanfecalmicrobiota
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Susceptibility to <italic toggle="yes">Campylobacter</italic> Infection Is Associated with the Species Composition of the Human Fecal Microbiota

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