Chitosan oligosaccharides alleviate acute heat stress-induced oxidative damage by activating ERK1/2-mediated HO-1 and GSH-Px gene expression in breast muscle of broilers

ABSTRACT: The purpose of this study was to evaluate the effects of chitosan oligosaccharides (COS) on acute heat stress (AHS) induced poor meat quality by alleviating oxidative damage through mitogen-activated protein kinase-nuclear factor-erythroid 2-related factor 2-antioxidant responsive element...

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Autores principales:	Qingqing Chang, Haoan Cai, Linlin Wei, Ruixia Lan
Formato:	article
Lenguaje:	EN
Publicado:	Elsevier 2022
Materias:	chitosan oligosaccharides acute heat stress meat quality antioxidant status MAPK-Nrf2-ARE signaling pathway Animal culture SF1-1100
Acceso en línea:	https://doaj.org/article/79e0678df4444215a04cd06f2d735f8a
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spelling	oai:doaj.org-article:79e0678df4444215a04cd06f2d735f8a2021-11-26T04:23:04ZChitosan oligosaccharides alleviate acute heat stress-induced oxidative damage by activating ERK1/2-mediated HO-1 and GSH-Px gene expression in breast muscle of broilers0032-579110.1016/j.psj.2021.101515https://doaj.org/article/79e0678df4444215a04cd06f2d735f8a2022-01-01T00:00:00Zhttp://www.sciencedirect.com/science/article/pii/S003257912100537Xhttps://doaj.org/toc/0032-5791ABSTRACT: The purpose of this study was to evaluate the effects of chitosan oligosaccharides (COS) on acute heat stress (AHS) induced poor meat quality by alleviating oxidative damage through mitogen-activated protein kinase-nuclear factor-erythroid 2-related factor 2-antioxidant responsive element (MAPK-Nrf2-ARE) signaling pathway. A total of 108 thirty-five-day-old Chinese indigenous broilers (Luhua chicken) was used for this 42-d experiment. The broilers were randomly allocated to 3 treatments: control group (CON), AHS group, and AHS with 400 mg/kg COS supplementation (AHS-C) group. Both CON and AHS groups given the basal diet, and the AHS-C group given the basal diet with 400 mg/kg COS supplementation. On d 42, broilers in the AHS and AHS-C groups treated with AHS (increasing temperature from 24 to 34°C in 2-h and held for another 2-h), and the CON group under normal temperature (24°C). AHS exposure elevated (P < 0.05) body temperature (rectal, comb, eyelids, and feet) of broilers, increased (P < 0.05) breast muscle lightness (L), drip loss, share force, hydrogen peroxide (H2O2) scavenging activity, reactive oxygen species (ROS) production, malondialdehyde (MDA) content, and catalase (CAT) activity, however, decreased (P < 0.05) pH45min, pH24h, redness (a), and relative expression of heme oxygenase-1 (HO-1). Compared to the AHS group, dietary COS supplementation increased (P < 0.05) breast muscle pH45min, pH24h, and a*, H2O2 scavenging activity, as well as relative expression of HO-1 and glutathione peroxidase (GSH-Px), however, decreased (P < 0.05) drip loss, share force, superoxide anion free radicals (O2•−) scavenging activity, ROS production, and MDA content. It was concluded that AHS impaired meat quality, which may be related to oxidative damage, as evidenced by increasing ROS production, MDA content, and decreasing the relative expression of HO-1. Dietary COS supplementation could effectively elevate the meat quality of broilers exposed to AHS via decreasing ROS production, activating the Nrf2 pathway, and Nrf2-mediated HO-1 and GSH-Px gene expression.Qingqing ChangHaoan CaiLinlin WeiRuixia LanElsevierarticlechitosan oligosaccharidesacute heat stressmeat qualityantioxidant statusMAPK-Nrf2-ARE signaling pathwayAnimal cultureSF1-1100ENPoultry Science, Vol 101, Iss 1, Pp 101515- (2022)
institution	DOAJ
collection	DOAJ
language	EN
topic	chitosan oligosaccharides acute heat stress meat quality antioxidant status MAPK-Nrf2-ARE signaling pathway Animal culture SF1-1100
spellingShingle	chitosan oligosaccharides acute heat stress meat quality antioxidant status MAPK-Nrf2-ARE signaling pathway Animal culture SF1-1100 Qingqing Chang Haoan Cai Linlin Wei Ruixia Lan Chitosan oligosaccharides alleviate acute heat stress-induced oxidative damage by activating ERK1/2-mediated HO-1 and GSH-Px gene expression in breast muscle of broilers
description	ABSTRACT: The purpose of this study was to evaluate the effects of chitosan oligosaccharides (COS) on acute heat stress (AHS) induced poor meat quality by alleviating oxidative damage through mitogen-activated protein kinase-nuclear factor-erythroid 2-related factor 2-antioxidant responsive element (MAPK-Nrf2-ARE) signaling pathway. A total of 108 thirty-five-day-old Chinese indigenous broilers (Luhua chicken) was used for this 42-d experiment. The broilers were randomly allocated to 3 treatments: control group (CON), AHS group, and AHS with 400 mg/kg COS supplementation (AHS-C) group. Both CON and AHS groups given the basal diet, and the AHS-C group given the basal diet with 400 mg/kg COS supplementation. On d 42, broilers in the AHS and AHS-C groups treated with AHS (increasing temperature from 24 to 34°C in 2-h and held for another 2-h), and the CON group under normal temperature (24°C). AHS exposure elevated (P < 0.05) body temperature (rectal, comb, eyelids, and feet) of broilers, increased (P < 0.05) breast muscle lightness (L), drip loss, share force, hydrogen peroxide (H2O2) scavenging activity, reactive oxygen species (ROS) production, malondialdehyde (MDA) content, and catalase (CAT) activity, however, decreased (P < 0.05) pH45min, pH24h, redness (a), and relative expression of heme oxygenase-1 (HO-1). Compared to the AHS group, dietary COS supplementation increased (P < 0.05) breast muscle pH45min, pH24h, and a*, H2O2 scavenging activity, as well as relative expression of HO-1 and glutathione peroxidase (GSH-Px), however, decreased (P < 0.05) drip loss, share force, superoxide anion free radicals (O2•−) scavenging activity, ROS production, and MDA content. It was concluded that AHS impaired meat quality, which may be related to oxidative damage, as evidenced by increasing ROS production, MDA content, and decreasing the relative expression of HO-1. Dietary COS supplementation could effectively elevate the meat quality of broilers exposed to AHS via decreasing ROS production, activating the Nrf2 pathway, and Nrf2-mediated HO-1 and GSH-Px gene expression.
format	article
author	Qingqing Chang Haoan Cai Linlin Wei Ruixia Lan
author_facet	Qingqing Chang Haoan Cai Linlin Wei Ruixia Lan
author_sort	Qingqing Chang
title	Chitosan oligosaccharides alleviate acute heat stress-induced oxidative damage by activating ERK1/2-mediated HO-1 and GSH-Px gene expression in breast muscle of broilers
title_short	Chitosan oligosaccharides alleviate acute heat stress-induced oxidative damage by activating ERK1/2-mediated HO-1 and GSH-Px gene expression in breast muscle of broilers
title_full	Chitosan oligosaccharides alleviate acute heat stress-induced oxidative damage by activating ERK1/2-mediated HO-1 and GSH-Px gene expression in breast muscle of broilers
title_fullStr	Chitosan oligosaccharides alleviate acute heat stress-induced oxidative damage by activating ERK1/2-mediated HO-1 and GSH-Px gene expression in breast muscle of broilers
title_full_unstemmed	Chitosan oligosaccharides alleviate acute heat stress-induced oxidative damage by activating ERK1/2-mediated HO-1 and GSH-Px gene expression in breast muscle of broilers
title_sort	chitosan oligosaccharides alleviate acute heat stress-induced oxidative damage by activating erk1/2-mediated ho-1 and gsh-px gene expression in breast muscle of broilers
publisher	Elsevier
publishDate	2022
url	https://doaj.org/article/79e0678df4444215a04cd06f2d735f8a
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Chitosan oligosaccharides alleviate acute heat stress-induced oxidative damage by activating ERK1/2-mediated HO-1 and GSH-Px gene expression in breast muscle of broilers

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