Effects of ulinastatin on global ischemia via brain pro-inflammation signal

Ulinastatin [urinary trypsin inhibitor (UTI)] plays an important role in the protection of organs against ischemic injury during severe inflammation. The purposes of this study were to examine the effects of UTI on the levels of pro-inflammatory cytokines (PICs) and protein expression of PIC recepto...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Jiang Xiao-Ming, Hu Jing-Hai, Wang Lu-Lu, Ma Chi, Wang Xu, Liu Xiao-Liang
Formato:	article
Lenguaje:	EN
Publicado:	De Gruyter 2016
Materias:	cardiac arrest cardiopulmonary resuscitation cortex cytokines hippocampus ulinastatin Neurosciences. Biological psychiatry. Neuropsychiatry RC321-571
Acceso en línea:	https://doaj.org/article/79e39bfcbb764dedb1273578e052b80a
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:79e39bfcbb764dedb1273578e052b80a
record_format	dspace
spelling	oai:doaj.org-article:79e39bfcbb764dedb1273578e052b80a2021-12-05T14:11:04ZEffects of ulinastatin on global ischemia via brain pro-inflammation signal2081-693610.1515/tnsci-2016-0023https://doaj.org/article/79e39bfcbb764dedb1273578e052b80a2016-01-01T00:00:00Zhttps://doi.org/10.1515/tnsci-2016-0023https://doaj.org/toc/2081-6936Ulinastatin [urinary trypsin inhibitor (UTI)] plays an important role in the protection of organs against ischemic injury during severe inflammation. The purposes of this study were to examine the effects of UTI on the levels of pro-inflammatory cytokines (PICs) and protein expression of PIC receptors in the neocortex and hippocampus CA1 region of rats after transient global ischemia induced via cardiac arrest (CA). Specifcally, interleukin-1β (IL-1β), interleukin-6 (IL-6) and tumor necrosis factor-α (TNF-α) were analyzed. CA was induced by asphyxia followed by cardiopulmonary resuscitation in rats. ELISA and western blot analysis were employed to determine PICs and their receptors in the neocortex and hippocampus. Our results show that IL-1β, IL-6 and TNF-α were significantly elevated in the neocortex and hippocampal CA1 field after CA. This was accompanied with an increase in PIC receptors, namely IL-1R, IL-6R and TNFR1. Systemic injection of UTI attenuated the amplification of PIC signal pathways in these brain regions. UTI also improved the modified Neurological Severity Score and brain tissue edema in CA rats. Notably, UTI resulted in an increase in survival of CA rats as compared to CA rats without treatment. In conclusion, UTI plays a beneficial role in modulating transient global ischemia induced by CA by altering PIC signal mechanisms, but further studies are needed to draw more firm conclusions.Jiang Xiao-MingHu Jing-HaiWang Lu-LuMa ChiWang XuLiu Xiao-LiangDe Gruyterarticlecardiac arrestcardiopulmonary resuscitationcortexcytokineshippocampusulinastatinNeurosciences. Biological psychiatry. NeuropsychiatryRC321-571ENTranslational Neuroscience, Vol 7, Iss 1, Pp 158-163 (2016)
institution	DOAJ
collection	DOAJ
language	EN
topic	cardiac arrest cardiopulmonary resuscitation cortex cytokines hippocampus ulinastatin Neurosciences. Biological psychiatry. Neuropsychiatry RC321-571
spellingShingle	cardiac arrest cardiopulmonary resuscitation cortex cytokines hippocampus ulinastatin Neurosciences. Biological psychiatry. Neuropsychiatry RC321-571 Jiang Xiao-Ming Hu Jing-Hai Wang Lu-Lu Ma Chi Wang Xu Liu Xiao-Liang Effects of ulinastatin on global ischemia via brain pro-inflammation signal
description	Ulinastatin [urinary trypsin inhibitor (UTI)] plays an important role in the protection of organs against ischemic injury during severe inflammation. The purposes of this study were to examine the effects of UTI on the levels of pro-inflammatory cytokines (PICs) and protein expression of PIC receptors in the neocortex and hippocampus CA1 region of rats after transient global ischemia induced via cardiac arrest (CA). Specifcally, interleukin-1β (IL-1β), interleukin-6 (IL-6) and tumor necrosis factor-α (TNF-α) were analyzed. CA was induced by asphyxia followed by cardiopulmonary resuscitation in rats. ELISA and western blot analysis were employed to determine PICs and their receptors in the neocortex and hippocampus. Our results show that IL-1β, IL-6 and TNF-α were significantly elevated in the neocortex and hippocampal CA1 field after CA. This was accompanied with an increase in PIC receptors, namely IL-1R, IL-6R and TNFR1. Systemic injection of UTI attenuated the amplification of PIC signal pathways in these brain regions. UTI also improved the modified Neurological Severity Score and brain tissue edema in CA rats. Notably, UTI resulted in an increase in survival of CA rats as compared to CA rats without treatment. In conclusion, UTI plays a beneficial role in modulating transient global ischemia induced by CA by altering PIC signal mechanisms, but further studies are needed to draw more firm conclusions.
format	article
author	Jiang Xiao-Ming Hu Jing-Hai Wang Lu-Lu Ma Chi Wang Xu Liu Xiao-Liang
author_facet	Jiang Xiao-Ming Hu Jing-Hai Wang Lu-Lu Ma Chi Wang Xu Liu Xiao-Liang
author_sort	Jiang Xiao-Ming
title	Effects of ulinastatin on global ischemia via brain pro-inflammation signal
title_short	Effects of ulinastatin on global ischemia via brain pro-inflammation signal
title_full	Effects of ulinastatin on global ischemia via brain pro-inflammation signal
title_fullStr	Effects of ulinastatin on global ischemia via brain pro-inflammation signal
title_full_unstemmed	Effects of ulinastatin on global ischemia via brain pro-inflammation signal
title_sort	effects of ulinastatin on global ischemia via brain pro-inflammation signal
publisher	De Gruyter
publishDate	2016
url	https://doaj.org/article/79e39bfcbb764dedb1273578e052b80a
work_keys_str_mv	AT jiangxiaoming effectsofulinastatinonglobalischemiaviabrainproinflammationsignal AT hujinghai effectsofulinastatinonglobalischemiaviabrainproinflammationsignal AT wanglulu effectsofulinastatinonglobalischemiaviabrainproinflammationsignal AT machi effectsofulinastatinonglobalischemiaviabrainproinflammationsignal AT wangxu effectsofulinastatinonglobalischemiaviabrainproinflammationsignal AT liuxiaoliang effectsofulinastatinonglobalischemiaviabrainproinflammationsignal
_version_	1718371429697716224

Effects of ulinastatin on global ischemia via brain pro-inflammation signal

Ejemplares similares