A portable reverse transcription recombinase polymerase amplification assay for rapid detection of foot-and-mouth disease virus.
Foot-and-mouth disease (FMD) is a trans-boundary viral disease of livestock, which causes huge economic losses and constitutes a serious infectious threat for livestock farming worldwide. Early diagnosis of FMD helps to diminish its impact by adequate outbreak management. In this study, we describe...
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2013
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oai:doaj.org-article:79e59bb5bd2649f3b8b368e6c15847942021-11-18T08:58:49ZA portable reverse transcription recombinase polymerase amplification assay for rapid detection of foot-and-mouth disease virus.1932-620310.1371/journal.pone.0071642https://doaj.org/article/79e59bb5bd2649f3b8b368e6c15847942013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23977101/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203Foot-and-mouth disease (FMD) is a trans-boundary viral disease of livestock, which causes huge economic losses and constitutes a serious infectious threat for livestock farming worldwide. Early diagnosis of FMD helps to diminish its impact by adequate outbreak management. In this study, we describe the development of a real-time reverse transcription recombinase polymerase amplification (RT-RPA) assay for the detection of FMD virus (FMDV). The FMDV RT-RPA design targeted the 3D gene of FMDV and a 260 nt molecular RNA standard was used for assay validation. The RT-RPA assay was fast (4-10 minutes) and the analytical sensitivity was determined at 1436 RNA molecules detected by probit regression analysis. The FMDV RT-RPA assay detected RNA prepared from all seven FMDV serotypes but did not detect classical swine fever virus or swine vesicular disease virus. The FMDV RT-RPA assay was used in the field during the recent FMD outbreak in Egypt. In clinical samples, reverse transcription polymerase chain reaction (RT-PCR) and RT-RPA showed a diagnostic sensitivity of 100% and 98%, respectively. In conclusion, FMDV RT-RPA was quicker and much easier to handle in the field than real-time RT-PCR. Thus RT-RPA could be easily implemented to perform diagnostics at quarantine stations or farms for rapid spot-of-infection detection.Ahmed Abd El WahedAyman El-DeebMohamed El-TholothHanaa Abd El KaderAbeer AhmedSayed HassanBernd HoffmannBernd HaasMohamed A ShalabyFrank T HufertManfred WeidmannPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 8, p e71642 (2013) |
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Medicine R Science Q |
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Medicine R Science Q Ahmed Abd El Wahed Ayman El-Deeb Mohamed El-Tholoth Hanaa Abd El Kader Abeer Ahmed Sayed Hassan Bernd Hoffmann Bernd Haas Mohamed A Shalaby Frank T Hufert Manfred Weidmann A portable reverse transcription recombinase polymerase amplification assay for rapid detection of foot-and-mouth disease virus. |
| description |
Foot-and-mouth disease (FMD) is a trans-boundary viral disease of livestock, which causes huge economic losses and constitutes a serious infectious threat for livestock farming worldwide. Early diagnosis of FMD helps to diminish its impact by adequate outbreak management. In this study, we describe the development of a real-time reverse transcription recombinase polymerase amplification (RT-RPA) assay for the detection of FMD virus (FMDV). The FMDV RT-RPA design targeted the 3D gene of FMDV and a 260 nt molecular RNA standard was used for assay validation. The RT-RPA assay was fast (4-10 minutes) and the analytical sensitivity was determined at 1436 RNA molecules detected by probit regression analysis. The FMDV RT-RPA assay detected RNA prepared from all seven FMDV serotypes but did not detect classical swine fever virus or swine vesicular disease virus. The FMDV RT-RPA assay was used in the field during the recent FMD outbreak in Egypt. In clinical samples, reverse transcription polymerase chain reaction (RT-PCR) and RT-RPA showed a diagnostic sensitivity of 100% and 98%, respectively. In conclusion, FMDV RT-RPA was quicker and much easier to handle in the field than real-time RT-PCR. Thus RT-RPA could be easily implemented to perform diagnostics at quarantine stations or farms for rapid spot-of-infection detection. |
| format |
article |
| author |
Ahmed Abd El Wahed Ayman El-Deeb Mohamed El-Tholoth Hanaa Abd El Kader Abeer Ahmed Sayed Hassan Bernd Hoffmann Bernd Haas Mohamed A Shalaby Frank T Hufert Manfred Weidmann |
| author_facet |
Ahmed Abd El Wahed Ayman El-Deeb Mohamed El-Tholoth Hanaa Abd El Kader Abeer Ahmed Sayed Hassan Bernd Hoffmann Bernd Haas Mohamed A Shalaby Frank T Hufert Manfred Weidmann |
| author_sort |
Ahmed Abd El Wahed |
| title |
A portable reverse transcription recombinase polymerase amplification assay for rapid detection of foot-and-mouth disease virus. |
| title_short |
A portable reverse transcription recombinase polymerase amplification assay for rapid detection of foot-and-mouth disease virus. |
| title_full |
A portable reverse transcription recombinase polymerase amplification assay for rapid detection of foot-and-mouth disease virus. |
| title_fullStr |
A portable reverse transcription recombinase polymerase amplification assay for rapid detection of foot-and-mouth disease virus. |
| title_full_unstemmed |
A portable reverse transcription recombinase polymerase amplification assay for rapid detection of foot-and-mouth disease virus. |
| title_sort |
portable reverse transcription recombinase polymerase amplification assay for rapid detection of foot-and-mouth disease virus. |
| publisher |
Public Library of Science (PLoS) |
| publishDate |
2013 |
| url |
https://doaj.org/article/79e59bb5bd2649f3b8b368e6c1584794 |
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