BEaTS-α an open access 3D printed device for in vitro electromechanical stimulation of human induced pluripotent stem cells

Abstract 3D printing was used to develop an open access device capable of simultaneous electrical and mechanical stimulation of human induced pluripotent stem cells in 6-well plates. The device was designed using Computer-Aided Design (CAD) and 3D printed with autoclavable, FDA-approved materials. T...

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Autores principales:	David Cortes, Christopher D. McTiernan, Marc Ruel, Walfre Franco, Cencen Chu, Wenbin Liang, Erik J. Suuronen, Emilio I. Alarcon
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2020
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/79edc5e6e22f4fa2a09f0979fc660122
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spelling	oai:doaj.org-article:79edc5e6e22f4fa2a09f0979fc6601222021-12-02T15:39:40ZBEaTS-α an open access 3D printed device for in vitro electromechanical stimulation of human induced pluripotent stem cells10.1038/s41598-020-67169-12045-2322https://doaj.org/article/79edc5e6e22f4fa2a09f0979fc6601222020-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-020-67169-1https://doaj.org/toc/2045-2322Abstract 3D printing was used to develop an open access device capable of simultaneous electrical and mechanical stimulation of human induced pluripotent stem cells in 6-well plates. The device was designed using Computer-Aided Design (CAD) and 3D printed with autoclavable, FDA-approved materials. The compact design of the device and materials selection allows for its use inside cell incubators working at high humidity without the risk of overheating or corrosion. Mechanical stimulation of cells was carried out through the cyclic deflection of flexible, translucent silicone membranes by means of a vacuum-controlled, open-access device. A rhythmic stimulation cycle was programmed to create a more physiologically relevant in vitro model. This mechanical stimulation was coupled and synchronized with in situ electrical stimuli. We assessed the capabilities of our device to support cardiac myocytes derived from human induced pluripotent stem cells, confirming that cells cultured under electromechanical stimulation presented a defined/mature cardiomyocyte phenotype. This 3D printed device provides a unique high-throughput in vitro system that combines both mechanical and electrical stimulation, and as such, we foresee it finding applications in the study of any electrically responsive tissue such as muscles and nerves.David CortesChristopher D. McTiernanMarc RuelWalfre FrancoCencen ChuWenbin LiangErik J. SuuronenEmilio I. AlarconNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 10, Iss 1, Pp 1-8 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q David Cortes Christopher D. McTiernan Marc Ruel Walfre Franco Cencen Chu Wenbin Liang Erik J. Suuronen Emilio I. Alarcon BEaTS-α an open access 3D printed device for in vitro electromechanical stimulation of human induced pluripotent stem cells
description	Abstract 3D printing was used to develop an open access device capable of simultaneous electrical and mechanical stimulation of human induced pluripotent stem cells in 6-well plates. The device was designed using Computer-Aided Design (CAD) and 3D printed with autoclavable, FDA-approved materials. The compact design of the device and materials selection allows for its use inside cell incubators working at high humidity without the risk of overheating or corrosion. Mechanical stimulation of cells was carried out through the cyclic deflection of flexible, translucent silicone membranes by means of a vacuum-controlled, open-access device. A rhythmic stimulation cycle was programmed to create a more physiologically relevant in vitro model. This mechanical stimulation was coupled and synchronized with in situ electrical stimuli. We assessed the capabilities of our device to support cardiac myocytes derived from human induced pluripotent stem cells, confirming that cells cultured under electromechanical stimulation presented a defined/mature cardiomyocyte phenotype. This 3D printed device provides a unique high-throughput in vitro system that combines both mechanical and electrical stimulation, and as such, we foresee it finding applications in the study of any electrically responsive tissue such as muscles and nerves.
format	article
author	David Cortes Christopher D. McTiernan Marc Ruel Walfre Franco Cencen Chu Wenbin Liang Erik J. Suuronen Emilio I. Alarcon
author_facet	David Cortes Christopher D. McTiernan Marc Ruel Walfre Franco Cencen Chu Wenbin Liang Erik J. Suuronen Emilio I. Alarcon
author_sort	David Cortes
title	BEaTS-α an open access 3D printed device for in vitro electromechanical stimulation of human induced pluripotent stem cells
title_short	BEaTS-α an open access 3D printed device for in vitro electromechanical stimulation of human induced pluripotent stem cells
title_full	BEaTS-α an open access 3D printed device for in vitro electromechanical stimulation of human induced pluripotent stem cells
title_fullStr	BEaTS-α an open access 3D printed device for in vitro electromechanical stimulation of human induced pluripotent stem cells
title_full_unstemmed	BEaTS-α an open access 3D printed device for in vitro electromechanical stimulation of human induced pluripotent stem cells
title_sort	beats-α an open access 3d printed device for in vitro electromechanical stimulation of human induced pluripotent stem cells
publisher	Nature Portfolio
publishDate	2020
url	https://doaj.org/article/79edc5e6e22f4fa2a09f0979fc660122
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BEaTS-α an open access 3D printed device for in vitro electromechanical stimulation of human induced pluripotent stem cells

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