Identification and characterization of microRNAs in ovary and testis of Nile tilapia (Oreochromis niloticus) by using solexa sequencing technology.

MicroRNAs (miRNAs) are endogenous non-coding small RNAs which play important roles in the regulation of gene expression by cleaving or inhibiting the translation of target gene transcripts. Thereinto, some specific miRNAs show regulatory activities in gonad development via translational control. In...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Jun Xiao, Huan Zhong, Yi Zhou, Fan Yu, Yun Gao, Yongju Luo, Zhanyang Tang, Zhongbao Guo, Enyan Guo, Xi Gan, Ming Zhang, Yaping Zhang
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2014
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/79f0e37f76184268b6b6c14575d32ed9
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:79f0e37f76184268b6b6c14575d32ed9
record_format	dspace
spelling	oai:doaj.org-article:79f0e37f76184268b6b6c14575d32ed92021-11-18T08:36:03ZIdentification and characterization of microRNAs in ovary and testis of Nile tilapia (Oreochromis niloticus) by using solexa sequencing technology.1932-620310.1371/journal.pone.0086821https://doaj.org/article/79f0e37f76184268b6b6c14575d32ed92014-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24466258/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203MicroRNAs (miRNAs) are endogenous non-coding small RNAs which play important roles in the regulation of gene expression by cleaving or inhibiting the translation of target gene transcripts. Thereinto, some specific miRNAs show regulatory activities in gonad development via translational control. In order to further understand the role of miRNA-mediated posttranscriptional regulation in Nile tilapia (Oreochromis niloticus) ovary and testis, two small RNA libraries of Nile tilapia were sequenced by Solexa small RNA deep sequencing methods. A total of 9,731,431 and 8,880,497 raw reads, representing 5,407,800 and 4,396,281 unique sequences were obtained from the sexually mature ovaries and testes, respectively. After comparing the small RNA sequences with the Rfam database, 1,432,210 reads in ovaries and 984,146 reads in testes were matched to the genome sequence of Nile tilapia. Bioinformatic analysis identified 764 mature miRNA, 209 miRNA-5p and 202 miRNA-3p were found in the two libraries, of which 525 known miRNAs are both expressed in the ovary and testis of Nile tilapia. Comparison of expression profiles of the testis, miR-727, miR-129 and miR-29 families were highly expressed in tilapia ovary. Additionally, miR-132, miR-212, miR-33a and miR-135b families, showed significant higher expression in testis compared with that in ovary. Furthermore, the expression patterns of the miRNAs were analyzed in different developmental stages of gonad. The result showed different expression patterns were observed during development of testis and ovary. In addition, the identification and characterization of differentially expressed miRNAs in the ovaries and testis of Nile tilapia provides important information on the role of miRNA in the regulation of the ovarian and testicular development and function. This data will be helpful to facilitate studies on the regulation of miRNAs during teleosts reproduction.Jun XiaoHuan ZhongYi ZhouFan YuYun GaoYongju LuoZhanyang TangZhongbao GuoEnyan GuoXi GanMing ZhangYaping ZhangPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 9, Iss 1, p e86821 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Jun Xiao Huan Zhong Yi Zhou Fan Yu Yun Gao Yongju Luo Zhanyang Tang Zhongbao Guo Enyan Guo Xi Gan Ming Zhang Yaping Zhang Identification and characterization of microRNAs in ovary and testis of Nile tilapia (Oreochromis niloticus) by using solexa sequencing technology.
description	MicroRNAs (miRNAs) are endogenous non-coding small RNAs which play important roles in the regulation of gene expression by cleaving or inhibiting the translation of target gene transcripts. Thereinto, some specific miRNAs show regulatory activities in gonad development via translational control. In order to further understand the role of miRNA-mediated posttranscriptional regulation in Nile tilapia (Oreochromis niloticus) ovary and testis, two small RNA libraries of Nile tilapia were sequenced by Solexa small RNA deep sequencing methods. A total of 9,731,431 and 8,880,497 raw reads, representing 5,407,800 and 4,396,281 unique sequences were obtained from the sexually mature ovaries and testes, respectively. After comparing the small RNA sequences with the Rfam database, 1,432,210 reads in ovaries and 984,146 reads in testes were matched to the genome sequence of Nile tilapia. Bioinformatic analysis identified 764 mature miRNA, 209 miRNA-5p and 202 miRNA-3p were found in the two libraries, of which 525 known miRNAs are both expressed in the ovary and testis of Nile tilapia. Comparison of expression profiles of the testis, miR-727, miR-129 and miR-29 families were highly expressed in tilapia ovary. Additionally, miR-132, miR-212, miR-33a and miR-135b families, showed significant higher expression in testis compared with that in ovary. Furthermore, the expression patterns of the miRNAs were analyzed in different developmental stages of gonad. The result showed different expression patterns were observed during development of testis and ovary. In addition, the identification and characterization of differentially expressed miRNAs in the ovaries and testis of Nile tilapia provides important information on the role of miRNA in the regulation of the ovarian and testicular development and function. This data will be helpful to facilitate studies on the regulation of miRNAs during teleosts reproduction.
format	article
author	Jun Xiao Huan Zhong Yi Zhou Fan Yu Yun Gao Yongju Luo Zhanyang Tang Zhongbao Guo Enyan Guo Xi Gan Ming Zhang Yaping Zhang
author_facet	Jun Xiao Huan Zhong Yi Zhou Fan Yu Yun Gao Yongju Luo Zhanyang Tang Zhongbao Guo Enyan Guo Xi Gan Ming Zhang Yaping Zhang
author_sort	Jun Xiao
title	Identification and characterization of microRNAs in ovary and testis of Nile tilapia (Oreochromis niloticus) by using solexa sequencing technology.
title_short	Identification and characterization of microRNAs in ovary and testis of Nile tilapia (Oreochromis niloticus) by using solexa sequencing technology.
title_full	Identification and characterization of microRNAs in ovary and testis of Nile tilapia (Oreochromis niloticus) by using solexa sequencing technology.
title_fullStr	Identification and characterization of microRNAs in ovary and testis of Nile tilapia (Oreochromis niloticus) by using solexa sequencing technology.
title_full_unstemmed	Identification and characterization of microRNAs in ovary and testis of Nile tilapia (Oreochromis niloticus) by using solexa sequencing technology.
title_sort	identification and characterization of micrornas in ovary and testis of nile tilapia (oreochromis niloticus) by using solexa sequencing technology.
publisher	Public Library of Science (PLoS)
publishDate	2014
url	https://doaj.org/article/79f0e37f76184268b6b6c14575d32ed9
work_keys_str_mv	AT junxiao identificationandcharacterizationofmicrornasinovaryandtestisofniletilapiaoreochromisniloticusbyusingsolexasequencingtechnology AT huanzhong identificationandcharacterizationofmicrornasinovaryandtestisofniletilapiaoreochromisniloticusbyusingsolexasequencingtechnology AT yizhou identificationandcharacterizationofmicrornasinovaryandtestisofniletilapiaoreochromisniloticusbyusingsolexasequencingtechnology AT fanyu identificationandcharacterizationofmicrornasinovaryandtestisofniletilapiaoreochromisniloticusbyusingsolexasequencingtechnology AT yungao identificationandcharacterizationofmicrornasinovaryandtestisofniletilapiaoreochromisniloticusbyusingsolexasequencingtechnology AT yongjuluo identificationandcharacterizationofmicrornasinovaryandtestisofniletilapiaoreochromisniloticusbyusingsolexasequencingtechnology AT zhanyangtang identificationandcharacterizationofmicrornasinovaryandtestisofniletilapiaoreochromisniloticusbyusingsolexasequencingtechnology AT zhongbaoguo identificationandcharacterizationofmicrornasinovaryandtestisofniletilapiaoreochromisniloticusbyusingsolexasequencingtechnology AT enyanguo identificationandcharacterizationofmicrornasinovaryandtestisofniletilapiaoreochromisniloticusbyusingsolexasequencingtechnology AT xigan identificationandcharacterizationofmicrornasinovaryandtestisofniletilapiaoreochromisniloticusbyusingsolexasequencingtechnology AT mingzhang identificationandcharacterizationofmicrornasinovaryandtestisofniletilapiaoreochromisniloticusbyusingsolexasequencingtechnology AT yapingzhang identificationandcharacterizationofmicrornasinovaryandtestisofniletilapiaoreochromisniloticusbyusingsolexasequencingtechnology
_version_	1718421570490204160

Identification and characterization of microRNAs in ovary and testis of Nile tilapia (Oreochromis niloticus) by using solexa sequencing technology.

Ejemplares similares