Effect of pore diameter on the elution behavior of analytes from thermoresponsive polymer grafted beads packed columns

Abstract Temperature-responsive chromatography using thermoresponsive polymers is innovative and can control analyte retention via column temperature. Analyte elution behavior in this type of chromatography depends on the modified thermoresponsive polymer and the structure of the base materials. In...

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Autores principales:	Kenichi Nagase, Yuta Umemoto, Hideko Kanazawa
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/79f5814de8224b5d8d68919a364a7677
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spelling	oai:doaj.org-article:79f5814de8224b5d8d68919a364a76772021-12-02T16:57:37ZEffect of pore diameter on the elution behavior of analytes from thermoresponsive polymer grafted beads packed columns10.1038/s41598-021-89165-92045-2322https://doaj.org/article/79f5814de8224b5d8d68919a364a76772021-05-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-89165-9https://doaj.org/toc/2045-2322Abstract Temperature-responsive chromatography using thermoresponsive polymers is innovative and can control analyte retention via column temperature. Analyte elution behavior in this type of chromatography depends on the modified thermoresponsive polymer and the structure of the base materials. In the present study, we examine the effect of the pore diameter of silica beads on analyte elution behavior in temperature-responsive chromatography. Poly(N-isopropylacrylamide-co-n-butyl methacrylate) hydrogel was applied to beads of various pore sizes: 7, 12, and 30 nm. Almost the same amount of copolymer hydrogel was applied to all beads, indicating that the efficiency of copolymer modification was independent of pore size. Analyte retention on prepared beads in a packed column was observed using steroids, benzodiazepines, and barbiturates as analytes. Analyte retention times increased with temperature on packed columns of 12- and 30-nm beads, whereas the column packed with 7-nm beads exhibited decreased retention times with increasing temperature. The difference in analyte elution behavior among the various pore sizes was attributed to analyte diffusion into the bead pores. These results demonstrate that bead pore diameter determines temperature-dependent elution behavior.Kenichi NagaseYuta UmemotoHideko KanazawaNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-11 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Kenichi Nagase Yuta Umemoto Hideko Kanazawa Effect of pore diameter on the elution behavior of analytes from thermoresponsive polymer grafted beads packed columns
description	Abstract Temperature-responsive chromatography using thermoresponsive polymers is innovative and can control analyte retention via column temperature. Analyte elution behavior in this type of chromatography depends on the modified thermoresponsive polymer and the structure of the base materials. In the present study, we examine the effect of the pore diameter of silica beads on analyte elution behavior in temperature-responsive chromatography. Poly(N-isopropylacrylamide-co-n-butyl methacrylate) hydrogel was applied to beads of various pore sizes: 7, 12, and 30 nm. Almost the same amount of copolymer hydrogel was applied to all beads, indicating that the efficiency of copolymer modification was independent of pore size. Analyte retention on prepared beads in a packed column was observed using steroids, benzodiazepines, and barbiturates as analytes. Analyte retention times increased with temperature on packed columns of 12- and 30-nm beads, whereas the column packed with 7-nm beads exhibited decreased retention times with increasing temperature. The difference in analyte elution behavior among the various pore sizes was attributed to analyte diffusion into the bead pores. These results demonstrate that bead pore diameter determines temperature-dependent elution behavior.
format	article
author	Kenichi Nagase Yuta Umemoto Hideko Kanazawa
author_facet	Kenichi Nagase Yuta Umemoto Hideko Kanazawa
author_sort	Kenichi Nagase
title	Effect of pore diameter on the elution behavior of analytes from thermoresponsive polymer grafted beads packed columns
title_short	Effect of pore diameter on the elution behavior of analytes from thermoresponsive polymer grafted beads packed columns
title_full	Effect of pore diameter on the elution behavior of analytes from thermoresponsive polymer grafted beads packed columns
title_fullStr	Effect of pore diameter on the elution behavior of analytes from thermoresponsive polymer grafted beads packed columns
title_full_unstemmed	Effect of pore diameter on the elution behavior of analytes from thermoresponsive polymer grafted beads packed columns
title_sort	effect of pore diameter on the elution behavior of analytes from thermoresponsive polymer grafted beads packed columns
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/79f5814de8224b5d8d68919a364a7677
work_keys_str_mv	AT kenichinagase effectofporediameterontheelutionbehaviorofanalytesfromthermoresponsivepolymergraftedbeadspackedcolumns AT yutaumemoto effectofporediameterontheelutionbehaviorofanalytesfromthermoresponsivepolymergraftedbeadspackedcolumns AT hidekokanazawa effectofporediameterontheelutionbehaviorofanalytesfromthermoresponsivepolymergraftedbeadspackedcolumns
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Effect of pore diameter on the elution behavior of analytes from thermoresponsive polymer grafted beads packed columns

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