A new primate model of hypophyseal dysfunction

Abstract For pituitary regenerative medicine, the creation of a hypophyseal model in monkeys is necessary to conduct future preclinical studies; however, previous studies reported that hypophysectomy in monkeys is not always safe or satisfactory. This study aimed to create a hypophyseal dysfunction...

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Autores principales:	Teppei Kawabata, Hidetaka Suga, Kazuhito Takeuchi, Yuichi Nagata, Mayu Sakakibara, Kaori Ushida, Chikafumi Ozone, Atsushi Enomoto, Ikuo Kawamoto, Iori Itagaki, Hideaki Tsuchiya, Hiroshi Arima, Toshihiko Wakabayashi
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/79f648092c304a05b8480fd4e15793f1
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spelling	oai:doaj.org-article:79f648092c304a05b8480fd4e15793f12021-12-02T16:53:19ZA new primate model of hypophyseal dysfunction10.1038/s41598-021-90209-32045-2322https://doaj.org/article/79f648092c304a05b8480fd4e15793f12021-05-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-90209-3https://doaj.org/toc/2045-2322Abstract For pituitary regenerative medicine, the creation of a hypophyseal model in monkeys is necessary to conduct future preclinical studies; however, previous studies reported that hypophysectomy in monkeys is not always safe or satisfactory. This study aimed to create a hypophyseal dysfunction model in a cynomolgus monkey using a safer surgical technique and establish the protocol of pituitary hormone replacement therapy for this model. Surgical resection of the pituitary gland of a 7.8-year-old healthy adult cynomolgus male monkey weighing 5.45 kg was performed to create a hypophyseal dysfunction model for future regenerative studies. Endoscopic transoral transsphenoidal surgery was used to perform hypophysectomy under navigation support. These procedures were useful for confirming total removal of the pituitary gland without additional bone removal and preventing complications such as cerebrospinal fluid leakage. Total removal was confirmed by pathological examination and computed tomography. Hypopituitarism was verified with endocrinological examinations including stimulation tests. Postoperatively, the monkey’s general condition of hypopituitarism was treated with hormone replacement therapy, resulting in long-term survival. The success of a minimally invasive and safe surgical method and long-term survival indicate the creation of a hypophyseal dysfunction model in a cynomolgus monkey; hence, this protocol can be employed in the future.Teppei KawabataHidetaka SugaKazuhito TakeuchiYuichi NagataMayu SakakibaraKaori UshidaChikafumi OzoneAtsushi EnomotoIkuo KawamotoIori ItagakiHideaki TsuchiyaHiroshi ArimaToshihiko WakabayashiNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-7 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Teppei Kawabata Hidetaka Suga Kazuhito Takeuchi Yuichi Nagata Mayu Sakakibara Kaori Ushida Chikafumi Ozone Atsushi Enomoto Ikuo Kawamoto Iori Itagaki Hideaki Tsuchiya Hiroshi Arima Toshihiko Wakabayashi A new primate model of hypophyseal dysfunction
description	Abstract For pituitary regenerative medicine, the creation of a hypophyseal model in monkeys is necessary to conduct future preclinical studies; however, previous studies reported that hypophysectomy in monkeys is not always safe or satisfactory. This study aimed to create a hypophyseal dysfunction model in a cynomolgus monkey using a safer surgical technique and establish the protocol of pituitary hormone replacement therapy for this model. Surgical resection of the pituitary gland of a 7.8-year-old healthy adult cynomolgus male monkey weighing 5.45 kg was performed to create a hypophyseal dysfunction model for future regenerative studies. Endoscopic transoral transsphenoidal surgery was used to perform hypophysectomy under navigation support. These procedures were useful for confirming total removal of the pituitary gland without additional bone removal and preventing complications such as cerebrospinal fluid leakage. Total removal was confirmed by pathological examination and computed tomography. Hypopituitarism was verified with endocrinological examinations including stimulation tests. Postoperatively, the monkey’s general condition of hypopituitarism was treated with hormone replacement therapy, resulting in long-term survival. The success of a minimally invasive and safe surgical method and long-term survival indicate the creation of a hypophyseal dysfunction model in a cynomolgus monkey; hence, this protocol can be employed in the future.
format	article
author	Teppei Kawabata Hidetaka Suga Kazuhito Takeuchi Yuichi Nagata Mayu Sakakibara Kaori Ushida Chikafumi Ozone Atsushi Enomoto Ikuo Kawamoto Iori Itagaki Hideaki Tsuchiya Hiroshi Arima Toshihiko Wakabayashi
author_facet	Teppei Kawabata Hidetaka Suga Kazuhito Takeuchi Yuichi Nagata Mayu Sakakibara Kaori Ushida Chikafumi Ozone Atsushi Enomoto Ikuo Kawamoto Iori Itagaki Hideaki Tsuchiya Hiroshi Arima Toshihiko Wakabayashi
author_sort	Teppei Kawabata
title	A new primate model of hypophyseal dysfunction
title_short	A new primate model of hypophyseal dysfunction
title_full	A new primate model of hypophyseal dysfunction
title_fullStr	A new primate model of hypophyseal dysfunction
title_full_unstemmed	A new primate model of hypophyseal dysfunction
title_sort	new primate model of hypophyseal dysfunction
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/79f648092c304a05b8480fd4e15793f1
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A new primate model of hypophyseal dysfunction

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