Effects of Lower Temperature on Expression and Biochemical Characteristics of HCV NS3 Antigen Recombinant Protein
The nonstructural antigen protein 3 of the hepatitis C virus (HCV NS3), commonly-used for HCV ELISA diagnosis, possesses protease and helicase activities. To prevent auto-degradation, a truncated NS3 protein was designed by removing the protease domain. Firstly, it was overexpressed in <i>E. c...
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Main Authors: | Chen-Ji Huang, Hwei-Ling Peng, Anil Kumar Patel, Reeta Rani Singhania, Cheng-Di Dong, Chih-Yu Cheng |
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Format: | article |
Language: | EN |
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MDPI AG
2021
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Online Access: | https://doaj.org/article/79f7a86df1cf4461a15e110a7ecee3f6 |
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