Characterization of the mechanism of inhibin α-subunit gene in mouse anterior pituitary cells by RNA interference.

Inhibin, a member of the transforming growth factor-β [TGF-β] superfamily, is a suppressor of follicle-stimulating hormone [FSH] release through pituitary-gonadal negative feedback loop to regulate follicular development. In this study, Inhibin α-subunit [Inha] gene was knocked down successfully in...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Li Han, Canjie Wu, Hasan Riaz, Liya Bai, Jianguo Chen, Yanhong Zhen, Aizhen Guo, Liguo Yang
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/79ff71a12c97426a97d339011e7ac555
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:79ff71a12c97426a97d339011e7ac555
record_format	dspace
spelling	oai:doaj.org-article:79ff71a12c97426a97d339011e7ac5552021-11-18T08:52:35ZCharacterization of the mechanism of inhibin α-subunit gene in mouse anterior pituitary cells by RNA interference.1932-620310.1371/journal.pone.0074596https://doaj.org/article/79ff71a12c97426a97d339011e7ac5552013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24098340/?tool=EBIhttps://doaj.org/toc/1932-6203Inhibin, a member of the transforming growth factor-β [TGF-β] superfamily, is a suppressor of follicle-stimulating hormone [FSH] release through pituitary-gonadal negative feedback loop to regulate follicular development. In this study, Inhibin α-subunit [Inha] gene was knocked down successfully in mice primary anterior pituitary cells at both transcriptional and translational levels by RNAi-Ready pSIREN-RetroQ-ZsGreen Vector mediated recombinant pshRNA vectors. The results indicated that inhibin silencing significantly promoted apoptosis by up-regulating Caspase-3, Bax and Bcl-2 genes without affecting p53 both at transcriptional and translational levels. Furthermore, it markedly impaired the progression of G1 phase of cell cycle and decreased the amount of cells in S phase [as detected by flow cytometry]. Inhibin silencing resulted in significant up-regulation of mRNA and protein expressions of Gondotropin releasing hormone receptors [GnRHR] and down-regulated mRNA levels of β-glycans with parellel change in the amount of its protein expression. Silencing of inhibin-a significantly increased [P<0.05] activin-β concentration without affecting FSH and LH levels in anterior pituitary cells. These findings revealed that up regulation of GnRH receptors by silencing inhibin a-subunit gene might increase the concentration of activin-β in the culture medium. Inhibin a silencing resulted in increased mRNA and protein expressions of inhibinβ which may demonstrate that both inhibin subunits co-participate in the regulation of reproductive events in anterior pituitary cells. This study concludes that inhibin is a broad regulatory marker in anterior pituitary cells by regulating apoptosis, cellular progression and simultaneously by vital fluctuations in the hormonal signaling.Li HanCanjie WuHasan RiazLiya BaiJianguo ChenYanhong ZhenAizhen GuoLiguo YangPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 10, p e74596 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Li Han Canjie Wu Hasan Riaz Liya Bai Jianguo Chen Yanhong Zhen Aizhen Guo Liguo Yang Characterization of the mechanism of inhibin α-subunit gene in mouse anterior pituitary cells by RNA interference.
description	Inhibin, a member of the transforming growth factor-β [TGF-β] superfamily, is a suppressor of follicle-stimulating hormone [FSH] release through pituitary-gonadal negative feedback loop to regulate follicular development. In this study, Inhibin α-subunit [Inha] gene was knocked down successfully in mice primary anterior pituitary cells at both transcriptional and translational levels by RNAi-Ready pSIREN-RetroQ-ZsGreen Vector mediated recombinant pshRNA vectors. The results indicated that inhibin silencing significantly promoted apoptosis by up-regulating Caspase-3, Bax and Bcl-2 genes without affecting p53 both at transcriptional and translational levels. Furthermore, it markedly impaired the progression of G1 phase of cell cycle and decreased the amount of cells in S phase [as detected by flow cytometry]. Inhibin silencing resulted in significant up-regulation of mRNA and protein expressions of Gondotropin releasing hormone receptors [GnRHR] and down-regulated mRNA levels of β-glycans with parellel change in the amount of its protein expression. Silencing of inhibin-a significantly increased [P<0.05] activin-β concentration without affecting FSH and LH levels in anterior pituitary cells. These findings revealed that up regulation of GnRH receptors by silencing inhibin a-subunit gene might increase the concentration of activin-β in the culture medium. Inhibin a silencing resulted in increased mRNA and protein expressions of inhibinβ which may demonstrate that both inhibin subunits co-participate in the regulation of reproductive events in anterior pituitary cells. This study concludes that inhibin is a broad regulatory marker in anterior pituitary cells by regulating apoptosis, cellular progression and simultaneously by vital fluctuations in the hormonal signaling.
format	article
author	Li Han Canjie Wu Hasan Riaz Liya Bai Jianguo Chen Yanhong Zhen Aizhen Guo Liguo Yang
author_facet	Li Han Canjie Wu Hasan Riaz Liya Bai Jianguo Chen Yanhong Zhen Aizhen Guo Liguo Yang
author_sort	Li Han
title	Characterization of the mechanism of inhibin α-subunit gene in mouse anterior pituitary cells by RNA interference.
title_short	Characterization of the mechanism of inhibin α-subunit gene in mouse anterior pituitary cells by RNA interference.
title_full	Characterization of the mechanism of inhibin α-subunit gene in mouse anterior pituitary cells by RNA interference.
title_fullStr	Characterization of the mechanism of inhibin α-subunit gene in mouse anterior pituitary cells by RNA interference.
title_full_unstemmed	Characterization of the mechanism of inhibin α-subunit gene in mouse anterior pituitary cells by RNA interference.
title_sort	characterization of the mechanism of inhibin α-subunit gene in mouse anterior pituitary cells by rna interference.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/79ff71a12c97426a97d339011e7ac555
work_keys_str_mv	AT lihan characterizationofthemechanismofinhibinasubunitgeneinmouseanteriorpituitarycellsbyrnainterference AT canjiewu characterizationofthemechanismofinhibinasubunitgeneinmouseanteriorpituitarycellsbyrnainterference AT hasanriaz characterizationofthemechanismofinhibinasubunitgeneinmouseanteriorpituitarycellsbyrnainterference AT liyabai characterizationofthemechanismofinhibinasubunitgeneinmouseanteriorpituitarycellsbyrnainterference AT jianguochen characterizationofthemechanismofinhibinasubunitgeneinmouseanteriorpituitarycellsbyrnainterference AT yanhongzhen characterizationofthemechanismofinhibinasubunitgeneinmouseanteriorpituitarycellsbyrnainterference AT aizhenguo characterizationofthemechanismofinhibinasubunitgeneinmouseanteriorpituitarycellsbyrnainterference AT liguoyang characterizationofthemechanismofinhibinasubunitgeneinmouseanteriorpituitarycellsbyrnainterference
_version_	1718421211383332864

Characterization of the mechanism of inhibin α-subunit gene in mouse anterior pituitary cells by RNA interference.

Ejemplares similares