Rapid detection and subtyping of human influenza A viruses and reassortants by pyrosequencing.

<h4>Background</h4>Given the continuing co-circulation of the 2009 H1N1 pandemic influenza A viruses with seasonal H3N2 viruses, rapid and reliable detection of newly emerging influenza reassortant viruses is important to enhance our influenza surveillance.<h4>Methodology/principal...

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Autores principales:	Yi-Mo Deng, Natalie Caldwell, Ian G Barr
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2011
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/7a009809ceeb47c0ac4c4477e5f29fa9
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spelling	oai:doaj.org-article:7a009809ceeb47c0ac4c4477e5f29fa92021-11-18T06:47:37ZRapid detection and subtyping of human influenza A viruses and reassortants by pyrosequencing.1932-620310.1371/journal.pone.0023400https://doaj.org/article/7a009809ceeb47c0ac4c4477e5f29fa92011-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/21886790/?tool=EBIhttps://doaj.org/toc/1932-6203<h4>Background</h4>Given the continuing co-circulation of the 2009 H1N1 pandemic influenza A viruses with seasonal H3N2 viruses, rapid and reliable detection of newly emerging influenza reassortant viruses is important to enhance our influenza surveillance.<h4>Methodology/principal findings</h4>A novel pyrosequencing assay was developed for the rapid identification and subtyping of potential human influenza A virus reassortants based on all eight gene segments of the virus. Except for HA and NA genes, one universal set of primers was used to amplify and subtype each of the six internal genes. With this method, all eight gene segments of 57 laboratory isolates and 17 original specimens of seasonal H1N1, H3N2 and 2009 H1N1 pandemic viruses were correctly matched with their corresponding subtypes. In addition, this method was shown to be capable of detecting reassortant viruses by correctly identifying the source of all 8 gene segments from three vaccine production reassortant viruses and three H1N2 viruses.<h4>Conclusions/significance</h4>In summary, this pyrosequencing assay is a sensitive and specific procedure for screening large numbers of viruses for reassortment events amongst the commonly circulating human influenza A viruses, which is more rapid and cheaper than using conventional sequencing approaches.Yi-Mo DengNatalie CaldwellIan G BarrPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 6, Iss 8, p e23400 (2011)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Yi-Mo Deng Natalie Caldwell Ian G Barr Rapid detection and subtyping of human influenza A viruses and reassortants by pyrosequencing.
description	<h4>Background</h4>Given the continuing co-circulation of the 2009 H1N1 pandemic influenza A viruses with seasonal H3N2 viruses, rapid and reliable detection of newly emerging influenza reassortant viruses is important to enhance our influenza surveillance.<h4>Methodology/principal findings</h4>A novel pyrosequencing assay was developed for the rapid identification and subtyping of potential human influenza A virus reassortants based on all eight gene segments of the virus. Except for HA and NA genes, one universal set of primers was used to amplify and subtype each of the six internal genes. With this method, all eight gene segments of 57 laboratory isolates and 17 original specimens of seasonal H1N1, H3N2 and 2009 H1N1 pandemic viruses were correctly matched with their corresponding subtypes. In addition, this method was shown to be capable of detecting reassortant viruses by correctly identifying the source of all 8 gene segments from three vaccine production reassortant viruses and three H1N2 viruses.<h4>Conclusions/significance</h4>In summary, this pyrosequencing assay is a sensitive and specific procedure for screening large numbers of viruses for reassortment events amongst the commonly circulating human influenza A viruses, which is more rapid and cheaper than using conventional sequencing approaches.
format	article
author	Yi-Mo Deng Natalie Caldwell Ian G Barr
author_facet	Yi-Mo Deng Natalie Caldwell Ian G Barr
author_sort	Yi-Mo Deng
title	Rapid detection and subtyping of human influenza A viruses and reassortants by pyrosequencing.
title_short	Rapid detection and subtyping of human influenza A viruses and reassortants by pyrosequencing.
title_full	Rapid detection and subtyping of human influenza A viruses and reassortants by pyrosequencing.
title_fullStr	Rapid detection and subtyping of human influenza A viruses and reassortants by pyrosequencing.
title_full_unstemmed	Rapid detection and subtyping of human influenza A viruses and reassortants by pyrosequencing.
title_sort	rapid detection and subtyping of human influenza a viruses and reassortants by pyrosequencing.
publisher	Public Library of Science (PLoS)
publishDate	2011
url	https://doaj.org/article/7a009809ceeb47c0ac4c4477e5f29fa9
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Rapid detection and subtyping of human influenza A viruses and reassortants by pyrosequencing.

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