Assessing the severity of laparotomy and partial hepatectomy in male rats-A multimodal approach.

This study assessed the postoperative severity after three different visceral surgical interventions in rats by using objective parameters pertaining to various disciplines. The objective was to evaluate whether the degree of severity increases with the invasiveness of the intervention and whether t...

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Autores principales:	Leonie Zieglowski, Anna Maria Kümmecke, Lisa Ernst, Rupert Palme, Ralf Weiskirchen, Steven R Talbot, René H Tolba
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/7a04a59c683c4f4a83c8e46397d655be
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spelling	oai:doaj.org-article:7a04a59c683c4f4a83c8e46397d655be2021-12-02T20:18:55ZAssessing the severity of laparotomy and partial hepatectomy in male rats-A multimodal approach.1932-620310.1371/journal.pone.0255175https://doaj.org/article/7a04a59c683c4f4a83c8e46397d655be2021-01-01T00:00:00Zhttps://doi.org/10.1371/journal.pone.0255175https://doaj.org/toc/1932-6203This study assessed the postoperative severity after three different visceral surgical interventions in rats by using objective parameters pertaining to various disciplines. The objective was to evaluate whether the degree of severity increases with the invasiveness of the intervention and whether this is in accordance with the EU Directive 2010/63. 136 adult male WistarHan rats were assigned to three groups: Sham-laparotomy (Sham) [7 days post-surgical survival time]; 50% partial hepatectomy (PH); 70% PH [PH groups with 1, 3, or 7 days post-surgical survival times]. Post-surgical severity assessment was performed via several multimodal assessment tools: I) model-specific score sheet focusing on body weight, general condition, spontaneous behavior, and the animals' willingness to move as well as on wound healing; II) Open Field tests evaluating the total distance and velocity an animal moved within 10 minutes and its rearing behavior during the test; III) telemetric data analyzing heart rate and blood pressure; and IV) analysis of blood (AST, ALT, and hemogram) and fecal samples (fecal corticosterone metabolites). Significant differences among the experimental groups and models were observed. We demonstrated that the Open Field test can detect significant changes in severity levels. Sham-laparotomy and removal of 50% of the liver mass were associated with comparable severity (mild-moderate); the severity parameters returned to baseline levels within seven days. Removal of 70% of the liver tissue seemed to be associated with a moderate severity grade and entailed a longer recovery period (>7 days) for complete regeneration. We recommend the use of Open Field tests as part of multimodal objective severity assessment.Leonie ZieglowskiAnna Maria KümmeckeLisa ErnstRupert PalmeRalf WeiskirchenSteven R TalbotRené H TolbaPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 16, Iss 8, p e0255175 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Leonie Zieglowski Anna Maria Kümmecke Lisa Ernst Rupert Palme Ralf Weiskirchen Steven R Talbot René H Tolba Assessing the severity of laparotomy and partial hepatectomy in male rats-A multimodal approach.
description	This study assessed the postoperative severity after three different visceral surgical interventions in rats by using objective parameters pertaining to various disciplines. The objective was to evaluate whether the degree of severity increases with the invasiveness of the intervention and whether this is in accordance with the EU Directive 2010/63. 136 adult male WistarHan rats were assigned to three groups: Sham-laparotomy (Sham) [7 days post-surgical survival time]; 50% partial hepatectomy (PH); 70% PH [PH groups with 1, 3, or 7 days post-surgical survival times]. Post-surgical severity assessment was performed via several multimodal assessment tools: I) model-specific score sheet focusing on body weight, general condition, spontaneous behavior, and the animals' willingness to move as well as on wound healing; II) Open Field tests evaluating the total distance and velocity an animal moved within 10 minutes and its rearing behavior during the test; III) telemetric data analyzing heart rate and blood pressure; and IV) analysis of blood (AST, ALT, and hemogram) and fecal samples (fecal corticosterone metabolites). Significant differences among the experimental groups and models were observed. We demonstrated that the Open Field test can detect significant changes in severity levels. Sham-laparotomy and removal of 50% of the liver mass were associated with comparable severity (mild-moderate); the severity parameters returned to baseline levels within seven days. Removal of 70% of the liver tissue seemed to be associated with a moderate severity grade and entailed a longer recovery period (>7 days) for complete regeneration. We recommend the use of Open Field tests as part of multimodal objective severity assessment.
format	article
author	Leonie Zieglowski Anna Maria Kümmecke Lisa Ernst Rupert Palme Ralf Weiskirchen Steven R Talbot René H Tolba
author_facet	Leonie Zieglowski Anna Maria Kümmecke Lisa Ernst Rupert Palme Ralf Weiskirchen Steven R Talbot René H Tolba
author_sort	Leonie Zieglowski
title	Assessing the severity of laparotomy and partial hepatectomy in male rats-A multimodal approach.
title_short	Assessing the severity of laparotomy and partial hepatectomy in male rats-A multimodal approach.
title_full	Assessing the severity of laparotomy and partial hepatectomy in male rats-A multimodal approach.
title_fullStr	Assessing the severity of laparotomy and partial hepatectomy in male rats-A multimodal approach.
title_full_unstemmed	Assessing the severity of laparotomy and partial hepatectomy in male rats-A multimodal approach.
title_sort	assessing the severity of laparotomy and partial hepatectomy in male rats-a multimodal approach.
publisher	Public Library of Science (PLoS)
publishDate	2021
url	https://doaj.org/article/7a04a59c683c4f4a83c8e46397d655be
work_keys_str_mv	AT leoniezieglowski assessingtheseverityoflaparotomyandpartialhepatectomyinmaleratsamultimodalapproach AT annamariakummecke assessingtheseverityoflaparotomyandpartialhepatectomyinmaleratsamultimodalapproach AT lisaernst assessingtheseverityoflaparotomyandpartialhepatectomyinmaleratsamultimodalapproach AT rupertpalme assessingtheseverityoflaparotomyandpartialhepatectomyinmaleratsamultimodalapproach AT ralfweiskirchen assessingtheseverityoflaparotomyandpartialhepatectomyinmaleratsamultimodalapproach AT stevenrtalbot assessingtheseverityoflaparotomyandpartialhepatectomyinmaleratsamultimodalapproach AT renehtolba assessingtheseverityoflaparotomyandpartialhepatectomyinmaleratsamultimodalapproach
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Assessing the severity of laparotomy and partial hepatectomy in male rats-A multimodal approach.

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