Automated home-cage monitoring as a potential measure of sickness behaviors and pain-like behaviors in LPS-treated mice.

The use of endotoxin, such as lipopolysaccharide (LPS) as a model of sickness behavior, has attracted recent attention. To objectively investigate sickness behavior along with its pain-like behaviors in LPS-treated mice, the behavioral measurement requires accurate methods, which reflects clinical r...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Hasriadi, Peththa Wadu Dasuni Wasana, Opa Vajragupta, Pornchai Rojsitthisak, Pasarapa Towiwat
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/7a102fa6b6b342e5941ff03457a51d56
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:7a102fa6b6b342e5941ff03457a51d56
record_format	dspace
spelling	oai:doaj.org-article:7a102fa6b6b342e5941ff03457a51d562021-12-02T20:19:23ZAutomated home-cage monitoring as a potential measure of sickness behaviors and pain-like behaviors in LPS-treated mice.1932-620310.1371/journal.pone.0256706https://doaj.org/article/7a102fa6b6b342e5941ff03457a51d562021-01-01T00:00:00Zhttps://doi.org/10.1371/journal.pone.0256706https://doaj.org/toc/1932-6203The use of endotoxin, such as lipopolysaccharide (LPS) as a model of sickness behavior, has attracted recent attention. To objectively investigate sickness behavior along with its pain-like behaviors in LPS-treated mice, the behavioral measurement requires accurate methods, which reflects clinical relevance. While reflexive pain response tests have been used for decades for pain assessment, its accuracy and clinical relevance remain problematic. Hence, we used automated home-cage monitoring LABORAS to evaluate spontaneous locomotive behaviors in LPS-induced mice. LPS-treated mice displayed sickness behaviors including pain-like behaviors in automated home-cage monitoring characterized by decreased mobile behaviors (climbing, locomotion, rearing) and increased immobility compared to that of the control group in both short- and long-term locomotive assessments. Here, in short-term measurement, both in the open-field test and automated home-cage monitoring, mice demonstrated impaired locomotive behaviors. We also assessed 24 h long-term locomotor activity in the home-cage system, which profiled the diurnal behaviors of LPS-stimulated mice. The results demonstrated significant behavioral impairment in LPS-stimulated mice compared to the control mice in both light and dark phases. However, the difference is more evident in the dark phase compared to the light phase owing to the nocturnal activity of mice. In addition, the administration of indomethacin as a pharmacological intervention improved sickness behaviors in the open-field test as well as automated home-cage monitoring, confirming that automated home-cage monitoring could be potentially useful in pharmacological screening. Together, our results demonstrate that automated home-cage monitoring could be a feasible alternative to conventional methods, such as the open-field test and combining several behavioral assessments may provide a better understanding of sickness behavior and pain-like behaviors in LPS-treated mice.HasriadiPeththa Wadu Dasuni WasanaOpa VajraguptaPornchai RojsitthisakPasarapa TowiwatPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 16, Iss 8, p e0256706 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Hasriadi Peththa Wadu Dasuni Wasana Opa Vajragupta Pornchai Rojsitthisak Pasarapa Towiwat Automated home-cage monitoring as a potential measure of sickness behaviors and pain-like behaviors in LPS-treated mice.
description	The use of endotoxin, such as lipopolysaccharide (LPS) as a model of sickness behavior, has attracted recent attention. To objectively investigate sickness behavior along with its pain-like behaviors in LPS-treated mice, the behavioral measurement requires accurate methods, which reflects clinical relevance. While reflexive pain response tests have been used for decades for pain assessment, its accuracy and clinical relevance remain problematic. Hence, we used automated home-cage monitoring LABORAS to evaluate spontaneous locomotive behaviors in LPS-induced mice. LPS-treated mice displayed sickness behaviors including pain-like behaviors in automated home-cage monitoring characterized by decreased mobile behaviors (climbing, locomotion, rearing) and increased immobility compared to that of the control group in both short- and long-term locomotive assessments. Here, in short-term measurement, both in the open-field test and automated home-cage monitoring, mice demonstrated impaired locomotive behaviors. We also assessed 24 h long-term locomotor activity in the home-cage system, which profiled the diurnal behaviors of LPS-stimulated mice. The results demonstrated significant behavioral impairment in LPS-stimulated mice compared to the control mice in both light and dark phases. However, the difference is more evident in the dark phase compared to the light phase owing to the nocturnal activity of mice. In addition, the administration of indomethacin as a pharmacological intervention improved sickness behaviors in the open-field test as well as automated home-cage monitoring, confirming that automated home-cage monitoring could be potentially useful in pharmacological screening. Together, our results demonstrate that automated home-cage monitoring could be a feasible alternative to conventional methods, such as the open-field test and combining several behavioral assessments may provide a better understanding of sickness behavior and pain-like behaviors in LPS-treated mice.
format	article
author	Hasriadi Peththa Wadu Dasuni Wasana Opa Vajragupta Pornchai Rojsitthisak Pasarapa Towiwat
author_facet	Hasriadi Peththa Wadu Dasuni Wasana Opa Vajragupta Pornchai Rojsitthisak Pasarapa Towiwat
author_sort	Hasriadi
title	Automated home-cage monitoring as a potential measure of sickness behaviors and pain-like behaviors in LPS-treated mice.
title_short	Automated home-cage monitoring as a potential measure of sickness behaviors and pain-like behaviors in LPS-treated mice.
title_full	Automated home-cage monitoring as a potential measure of sickness behaviors and pain-like behaviors in LPS-treated mice.
title_fullStr	Automated home-cage monitoring as a potential measure of sickness behaviors and pain-like behaviors in LPS-treated mice.
title_full_unstemmed	Automated home-cage monitoring as a potential measure of sickness behaviors and pain-like behaviors in LPS-treated mice.
title_sort	automated home-cage monitoring as a potential measure of sickness behaviors and pain-like behaviors in lps-treated mice.
publisher	Public Library of Science (PLoS)
publishDate	2021
url	https://doaj.org/article/7a102fa6b6b342e5941ff03457a51d56
work_keys_str_mv	AT hasriadi automatedhomecagemonitoringasapotentialmeasureofsicknessbehaviorsandpainlikebehaviorsinlpstreatedmice AT peththawadudasuniwasana automatedhomecagemonitoringasapotentialmeasureofsicknessbehaviorsandpainlikebehaviorsinlpstreatedmice AT opavajragupta automatedhomecagemonitoringasapotentialmeasureofsicknessbehaviorsandpainlikebehaviorsinlpstreatedmice AT pornchairojsitthisak automatedhomecagemonitoringasapotentialmeasureofsicknessbehaviorsandpainlikebehaviorsinlpstreatedmice AT pasarapatowiwat automatedhomecagemonitoringasapotentialmeasureofsicknessbehaviorsandpainlikebehaviorsinlpstreatedmice
_version_	1718374234576650240

Automated home-cage monitoring as a potential measure of sickness behaviors and pain-like behaviors in LPS-treated mice.

Ejemplares similares