Effects of Inositol Supplementation in Sperm Extender on the Quality of Cryopreserved Mesopotamian Catfish (<i>Silurus triostegus</i>, H. 1843) Sperm

In this study, the effects of supplemented inositol on sperm extenders were examined on the spermatozoa motility rate and duration, total antioxidant and oxidant status, apoptotic spermatozoa and DNA damage, during the sperm post-thaw process of Mesopotamian Catfish (<i>Silurus triostegus</...

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Autores principales:	Zafer Doğu, Erdinç Şahinöz, Faruk Aral, İsmail Koyuncu, Özgür Yüksekdağ
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	apoptosis cryopreservation DNA damage oxidative stress <i>Silurus triostegus</i> sperm Veterinary medicine SF600-1100 Zoology QL1-991
Acceso en línea:	https://doaj.org/article/7a11d84c6ea2422d9d98e841ab333066
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spelling	oai:doaj.org-article:7a11d84c6ea2422d9d98e841ab3330662021-11-25T16:13:53ZEffects of Inositol Supplementation in Sperm Extender on the Quality of Cryopreserved Mesopotamian Catfish (<i>Silurus triostegus</i>, H. 1843) Sperm10.3390/ani111130292076-2615https://doaj.org/article/7a11d84c6ea2422d9d98e841ab3330662021-10-01T00:00:00Zhttps://www.mdpi.com/2076-2615/11/11/3029https://doaj.org/toc/2076-2615In this study, the effects of supplemented inositol on sperm extenders were examined on the spermatozoa motility rate and duration, total antioxidant and oxidant status, apoptotic spermatozoa and DNA damage, during the sperm post-thaw process of Mesopotamian Catfish (<i>Silurus triostegus</i>, H. 1843). The semen was frozen in diluents containing different inositol concentrations (5, 10, 20 and 40 mg). Increasing levels of inositol linearly improved the spermatozoa motility rate and duration significantly (<i>p</i> < 0.05). MDA and TOS were linearly decreased, however, TAS and GSH linearly increased (<i>p</i> < 0.05). The increasing inositol levels resulted in a linear and quadratic decrease in DNA damage in the comet assay, 8-hydroxydeoxyguanosine and the determined percentage of apoptotic spermatozoa (<i>p</i> < 0.05). These results suggest that there are many positive effects of the use of supplemental inositol on enhancing sperm cryopreservation efficiency in <i>Silurus triostegus</i>.Zafer DoğuErdinç ŞahinözFaruk Aralİsmail KoyuncuÖzgür YüksekdağMDPI AGarticleapoptosiscryopreservationDNA damageoxidative stress<i>Silurus triostegus</i>spermVeterinary medicineSF600-1100ZoologyQL1-991ENAnimals, Vol 11, Iss 3029, p 3029 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	apoptosis cryopreservation DNA damage oxidative stress <i>Silurus triostegus</i> sperm Veterinary medicine SF600-1100 Zoology QL1-991
spellingShingle	apoptosis cryopreservation DNA damage oxidative stress <i>Silurus triostegus</i> sperm Veterinary medicine SF600-1100 Zoology QL1-991 Zafer Doğu Erdinç Şahinöz Faruk Aral İsmail Koyuncu Özgür Yüksekdağ Effects of Inositol Supplementation in Sperm Extender on the Quality of Cryopreserved Mesopotamian Catfish (<i>Silurus triostegus</i>, H. 1843) Sperm
description	In this study, the effects of supplemented inositol on sperm extenders were examined on the spermatozoa motility rate and duration, total antioxidant and oxidant status, apoptotic spermatozoa and DNA damage, during the sperm post-thaw process of Mesopotamian Catfish (<i>Silurus triostegus</i>, H. 1843). The semen was frozen in diluents containing different inositol concentrations (5, 10, 20 and 40 mg). Increasing levels of inositol linearly improved the spermatozoa motility rate and duration significantly (<i>p</i> < 0.05). MDA and TOS were linearly decreased, however, TAS and GSH linearly increased (<i>p</i> < 0.05). The increasing inositol levels resulted in a linear and quadratic decrease in DNA damage in the comet assay, 8-hydroxydeoxyguanosine and the determined percentage of apoptotic spermatozoa (<i>p</i> < 0.05). These results suggest that there are many positive effects of the use of supplemental inositol on enhancing sperm cryopreservation efficiency in <i>Silurus triostegus</i>.
format	article
author	Zafer Doğu Erdinç Şahinöz Faruk Aral İsmail Koyuncu Özgür Yüksekdağ
author_facet	Zafer Doğu Erdinç Şahinöz Faruk Aral İsmail Koyuncu Özgür Yüksekdağ
author_sort	Zafer Doğu
title	Effects of Inositol Supplementation in Sperm Extender on the Quality of Cryopreserved Mesopotamian Catfish (<i>Silurus triostegus</i>, H. 1843) Sperm
title_short	Effects of Inositol Supplementation in Sperm Extender on the Quality of Cryopreserved Mesopotamian Catfish (<i>Silurus triostegus</i>, H. 1843) Sperm
title_full	Effects of Inositol Supplementation in Sperm Extender on the Quality of Cryopreserved Mesopotamian Catfish (<i>Silurus triostegus</i>, H. 1843) Sperm
title_fullStr	Effects of Inositol Supplementation in Sperm Extender on the Quality of Cryopreserved Mesopotamian Catfish (<i>Silurus triostegus</i>, H. 1843) Sperm
title_full_unstemmed	Effects of Inositol Supplementation in Sperm Extender on the Quality of Cryopreserved Mesopotamian Catfish (<i>Silurus triostegus</i>, H. 1843) Sperm
title_sort	effects of inositol supplementation in sperm extender on the quality of cryopreserved mesopotamian catfish (<i>silurus triostegus</i>, h. 1843) sperm
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/7a11d84c6ea2422d9d98e841ab333066
work_keys_str_mv	AT zaferdogu effectsofinositolsupplementationinspermextenderonthequalityofcryopreservedmesopotamiancatfishisilurustriostegusih1843sperm AT erdincsahinoz effectsofinositolsupplementationinspermextenderonthequalityofcryopreservedmesopotamiancatfishisilurustriostegusih1843sperm AT farukaral effectsofinositolsupplementationinspermextenderonthequalityofcryopreservedmesopotamiancatfishisilurustriostegusih1843sperm AT ismailkoyuncu effectsofinositolsupplementationinspermextenderonthequalityofcryopreservedmesopotamiancatfishisilurustriostegusih1843sperm AT ozguryuksekdag effectsofinositolsupplementationinspermextenderonthequalityofcryopreservedmesopotamiancatfishisilurustriostegusih1843sperm
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Effects of Inositol Supplementation in Sperm Extender on the Quality of Cryopreserved Mesopotamian Catfish (<i>Silurus triostegus</i>, H. 1843) Sperm

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