Verification Of Molecular And Conventional Techniques Used In The Diagnosis Of Equine Herpes Virus In Some Egyptian Governorates

Equid herpesviruses (EHVs) affect equine health and can cause signiﬁcant economic losses to the equine industry worldwide. In the current study, the circulation of two infectious equid herpesviruses (EHV-1 and EHV-4) among different horse populations in some farms was monitored. In the present study...

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Autores principales:	Morcos Ibrahim Yanni, Ebtsam, A. Abouelyazeed, Hala Abdelrehim Ali, Nadia Maher Hanna
Formato:	article
Lenguaje:	EN
Publicado:	Egyptian Society for Animal Management 2021
Materias:	dna elisa ehv-1 equine herpesvirus Zoology QL1-991 Veterinary medicine SF600-1100 Animal biochemistry QP501-801
Acceso en línea:	https://dx.doi.org/10.21608/javs.2021.140052 https://doaj.org/article/7a135de79e4d4ca8a23a645b84a5af80
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spelling	oai:doaj.org-article:7a135de79e4d4ca8a23a645b84a5af802021-12-02T11:45:16ZVerification Of Molecular And Conventional Techniques Used In The Diagnosis Of Equine Herpes Virus In Some Egyptian Governorateshttps://dx.doi.org/10.21608/javs.2021.1400521687-40722090-3308https://doaj.org/article/7a135de79e4d4ca8a23a645b84a5af802021-01-01T00:00:00Zhttps://javs.journals.ekb.eg/article_140052.htmlhttps://doaj.org/toc/1687-4072https://doaj.org/toc/2090-3308Equid herpesviruses (EHVs) affect equine health and can cause signiﬁcant economic losses to the equine industry worldwide. In the current study, the circulation of two infectious equid herpesviruses (EHV-1 and EHV-4) among different horse populations in some farms was monitored. In the present study, 50 samples of nasal secretions and tissue homogenates from neurological disease cases, abortion, neonatal foal deaths, and 36 serum samples. Samples of swabs and organs inoculated in embryonated chiken egg and Madin darby bovine kidney cell line. 29samples were positive in egg injection but no detected CPE in cell line for three passages. DNA was extracted and subjected to conventional PCR to detect the two herpesviruses' presence using speciﬁc primers. Three isolates of EHV-1 and four were detected. One EHV-1 and two EHV-4 were subjected to phylogenetic analysis. Phylogenetic analysis confirmed the existence of the isolated EHV-1 and EHV-4. They were more closely related to other previously isolated EHV-1 and EHV-4 from Egypt and other countries. Antibodies against EHV-1 and EHV-4 were tested using ELISA. The results showed that EHV-1 and EHV-4 are endemic and can be a continuous threat for horses in the absence of vaccination programs and frequent virus reactivation.Morcos Ibrahim YanniEbtsam, A. AbouelyazeedHala Abdelrehim AliNadia Maher HannaEgyptian Society for Animal Managementarticlednaelisaehv-1equineherpesvirusZoologyQL1-991Veterinary medicineSF600-1100Animal biochemistryQP501-801ENJournal of Applied Veterinary Sciences, Vol 6, Iss 1, Pp 1-8 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	dna elisa ehv-1 equine herpesvirus Zoology QL1-991 Veterinary medicine SF600-1100 Animal biochemistry QP501-801
spellingShingle	dna elisa ehv-1 equine herpesvirus Zoology QL1-991 Veterinary medicine SF600-1100 Animal biochemistry QP501-801 Morcos Ibrahim Yanni Ebtsam, A. Abouelyazeed Hala Abdelrehim Ali Nadia Maher Hanna Verification Of Molecular And Conventional Techniques Used In The Diagnosis Of Equine Herpes Virus In Some Egyptian Governorates
description	Equid herpesviruses (EHVs) affect equine health and can cause signiﬁcant economic losses to the equine industry worldwide. In the current study, the circulation of two infectious equid herpesviruses (EHV-1 and EHV-4) among different horse populations in some farms was monitored. In the present study, 50 samples of nasal secretions and tissue homogenates from neurological disease cases, abortion, neonatal foal deaths, and 36 serum samples. Samples of swabs and organs inoculated in embryonated chiken egg and Madin darby bovine kidney cell line. 29samples were positive in egg injection but no detected CPE in cell line for three passages. DNA was extracted and subjected to conventional PCR to detect the two herpesviruses' presence using speciﬁc primers. Three isolates of EHV-1 and four were detected. One EHV-1 and two EHV-4 were subjected to phylogenetic analysis. Phylogenetic analysis confirmed the existence of the isolated EHV-1 and EHV-4. They were more closely related to other previously isolated EHV-1 and EHV-4 from Egypt and other countries. Antibodies against EHV-1 and EHV-4 were tested using ELISA. The results showed that EHV-1 and EHV-4 are endemic and can be a continuous threat for horses in the absence of vaccination programs and frequent virus reactivation.
format	article
author	Morcos Ibrahim Yanni Ebtsam, A. Abouelyazeed Hala Abdelrehim Ali Nadia Maher Hanna
author_facet	Morcos Ibrahim Yanni Ebtsam, A. Abouelyazeed Hala Abdelrehim Ali Nadia Maher Hanna
author_sort	Morcos Ibrahim Yanni
title	Verification Of Molecular And Conventional Techniques Used In The Diagnosis Of Equine Herpes Virus In Some Egyptian Governorates
title_short	Verification Of Molecular And Conventional Techniques Used In The Diagnosis Of Equine Herpes Virus In Some Egyptian Governorates
title_full	Verification Of Molecular And Conventional Techniques Used In The Diagnosis Of Equine Herpes Virus In Some Egyptian Governorates
title_fullStr	Verification Of Molecular And Conventional Techniques Used In The Diagnosis Of Equine Herpes Virus In Some Egyptian Governorates
title_full_unstemmed	Verification Of Molecular And Conventional Techniques Used In The Diagnosis Of Equine Herpes Virus In Some Egyptian Governorates
title_sort	verification of molecular and conventional techniques used in the diagnosis of equine herpes virus in some egyptian governorates
publisher	Egyptian Society for Animal Management
publishDate	2021
url	https://dx.doi.org/10.21608/javs.2021.140052 https://doaj.org/article/7a135de79e4d4ca8a23a645b84a5af80
work_keys_str_mv	AT morcosibrahimyanni verificationofmolecularandconventionaltechniquesusedinthediagnosisofequineherpesvirusinsomeegyptiangovernorates AT ebtsamaabouelyazeed verificationofmolecularandconventionaltechniquesusedinthediagnosisofequineherpesvirusinsomeegyptiangovernorates AT halaabdelrehimali verificationofmolecularandconventionaltechniquesusedinthediagnosisofequineherpesvirusinsomeegyptiangovernorates AT nadiamaherhanna verificationofmolecularandconventionaltechniquesusedinthediagnosisofequineherpesvirusinsomeegyptiangovernorates
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Verification Of Molecular And Conventional Techniques Used In The Diagnosis Of Equine Herpes Virus In Some Egyptian Governorates

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