Phylogenetic Group B2 Expressed Significant Biofilm Formation among Drug Resistant Uropathogenic Escherichia coli

Biofilm is an important virulent marker attributed to the development of urinary tract infections (UTIs) by uropathogenic E. coli (UPEC). Drug-resistant and biofilm-producing UPEC are highly problematic causing catheter-associated or recurrent UTIs with significant morbidity and mortality. The aim o...

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Autores principales:	Saima Javed, Zulfiqar Ali Mirani, Zaid Ahmed Pirzada
Formato:	article
Lenguaje:	EN
Publicado:	Taylor & Francis Group 2021
Materias:	urinary tract infections (utis) biofilm formation uropathogenic e. coli phylogenetic groups Medicine R
Acceso en línea:	https://doaj.org/article/7a14d63b7f9c4b9f8b63699da33c2cb0
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spelling	oai:doaj.org-article:7a14d63b7f9c4b9f8b63699da33c2cb02021-11-26T11:19:48ZPhylogenetic Group B2 Expressed Significant Biofilm Formation among Drug Resistant Uropathogenic Escherichia coli1993-28201819-635710.1080/19932820.2020.1845444https://doaj.org/article/7a14d63b7f9c4b9f8b63699da33c2cb02021-01-01T00:00:00Zhttp://dx.doi.org/10.1080/19932820.2020.1845444https://doaj.org/toc/1993-2820https://doaj.org/toc/1819-6357Biofilm is an important virulent marker attributed to the development of urinary tract infections (UTIs) by uropathogenic E. coli (UPEC). Drug-resistant and biofilm-producing UPEC are highly problematic causing catheter-associated or recurrent UTIs with significant morbidity and mortality. The aim of the current study was to investigate the prevalence of biofilm formation and phylogenetic groups in drug-resistant UPEC to predict their ability to cause disease. This prospective study was conducted at the Department of Microbiology, University of Karachi from January to June 2019. A total of 50 highly drug-resistant UPEC were selected for this study. UPEC isolates were screened to form biofilm by Congo-red agar (CRA) and microtiter plate (MTP) technique. The representative biofilm-producing isolates were analysed by scanning electron microscopy (SEM) monitoring. Phylogenetic analysis was done by PCR method based on two preserved genes; chuA, yjaA and TspE4-C2 DNA fragment. On CRA 34 (68%) UPEC were slime producers, while on MTP 20 (40%) were strong biofilm producers, 19 (38%) moderate and 11 (22%) were low to negligible biofilm producers. Molecular typing confirmed that phylogenetic group B2 was prevalent in drug resistant UPEC strains. Pathogenic strains belonged to phylogenetic group B2 and D were found to have greater biofilm forming ability as compare to non-pathogenic commensal strains that belonged to phylogenetic group A. Our results indicate that biofilm formation vary in drug resistant UPEC belonged to different phylogenetic groups. This study indicates possible link between in vitro biofilm formation and phylogenetic groups of UPEC, therefore this knowledge might be helpful to predict the pathogenic potential of UPEC and help design strategies for controlling UTIs.Saima JavedZulfiqar Ali MiraniZaid Ahmed PirzadaTaylor & Francis Grouparticleurinary tract infections (utis)biofilm formationuropathogenic e. coliphylogenetic groupsMedicineRENLibyan Journal of Medicine, Vol 16, Iss 1 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	urinary tract infections (utis) biofilm formation uropathogenic e. coli phylogenetic groups Medicine R
spellingShingle	urinary tract infections (utis) biofilm formation uropathogenic e. coli phylogenetic groups Medicine R Saima Javed Zulfiqar Ali Mirani Zaid Ahmed Pirzada Phylogenetic Group B2 Expressed Significant Biofilm Formation among Drug Resistant Uropathogenic Escherichia coli
description	Biofilm is an important virulent marker attributed to the development of urinary tract infections (UTIs) by uropathogenic E. coli (UPEC). Drug-resistant and biofilm-producing UPEC are highly problematic causing catheter-associated or recurrent UTIs with significant morbidity and mortality. The aim of the current study was to investigate the prevalence of biofilm formation and phylogenetic groups in drug-resistant UPEC to predict their ability to cause disease. This prospective study was conducted at the Department of Microbiology, University of Karachi from January to June 2019. A total of 50 highly drug-resistant UPEC were selected for this study. UPEC isolates were screened to form biofilm by Congo-red agar (CRA) and microtiter plate (MTP) technique. The representative biofilm-producing isolates were analysed by scanning electron microscopy (SEM) monitoring. Phylogenetic analysis was done by PCR method based on two preserved genes; chuA, yjaA and TspE4-C2 DNA fragment. On CRA 34 (68%) UPEC were slime producers, while on MTP 20 (40%) were strong biofilm producers, 19 (38%) moderate and 11 (22%) were low to negligible biofilm producers. Molecular typing confirmed that phylogenetic group B2 was prevalent in drug resistant UPEC strains. Pathogenic strains belonged to phylogenetic group B2 and D were found to have greater biofilm forming ability as compare to non-pathogenic commensal strains that belonged to phylogenetic group A. Our results indicate that biofilm formation vary in drug resistant UPEC belonged to different phylogenetic groups. This study indicates possible link between in vitro biofilm formation and phylogenetic groups of UPEC, therefore this knowledge might be helpful to predict the pathogenic potential of UPEC and help design strategies for controlling UTIs.
format	article
author	Saima Javed Zulfiqar Ali Mirani Zaid Ahmed Pirzada
author_facet	Saima Javed Zulfiqar Ali Mirani Zaid Ahmed Pirzada
author_sort	Saima Javed
title	Phylogenetic Group B2 Expressed Significant Biofilm Formation among Drug Resistant Uropathogenic Escherichia coli
title_short	Phylogenetic Group B2 Expressed Significant Biofilm Formation among Drug Resistant Uropathogenic Escherichia coli
title_full	Phylogenetic Group B2 Expressed Significant Biofilm Formation among Drug Resistant Uropathogenic Escherichia coli
title_fullStr	Phylogenetic Group B2 Expressed Significant Biofilm Formation among Drug Resistant Uropathogenic Escherichia coli
title_full_unstemmed	Phylogenetic Group B2 Expressed Significant Biofilm Formation among Drug Resistant Uropathogenic Escherichia coli
title_sort	phylogenetic group b2 expressed significant biofilm formation among drug resistant uropathogenic escherichia coli
publisher	Taylor & Francis Group
publishDate	2021
url	https://doaj.org/article/7a14d63b7f9c4b9f8b63699da33c2cb0
work_keys_str_mv	AT saimajaved phylogeneticgroupb2expressedsignificantbiofilmformationamongdrugresistanturopathogenicescherichiacoli AT zulfiqaralimirani phylogeneticgroupb2expressedsignificantbiofilmformationamongdrugresistanturopathogenicescherichiacoli AT zaidahmedpirzada phylogeneticgroupb2expressedsignificantbiofilmformationamongdrugresistanturopathogenicescherichiacoli
_version_	1718409480976203776

Phylogenetic Group B2 Expressed Significant Biofilm Formation among Drug Resistant Uropathogenic Escherichia coli

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