From metabolome to phenotype: GC-MS metabolomics of developing mutant barley seeds reveals effects of growth, temperature and genotype

Abstract The development of crop varieties tolerant to growth temperature fluctuations and improved nutritional value is crucial due to climate change and global population growth. This study investigated the metabolite patterns of developing barley seed as a function of genotype and growth temperat...

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Autores principales:	Bekzod Khakimov, Morten Arendt Rasmussen, Rubini Maya Kannangara, Birthe Møller Jespersen, Lars Munck, Søren Balling Engelsen
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/7a1c13f3101641938fc35a1308ea83a5
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spelling	oai:doaj.org-article:7a1c13f3101641938fc35a1308ea83a52021-12-02T15:05:53ZFrom metabolome to phenotype: GC-MS metabolomics of developing mutant barley seeds reveals effects of growth, temperature and genotype10.1038/s41598-017-08129-02045-2322https://doaj.org/article/7a1c13f3101641938fc35a1308ea83a52017-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-08129-0https://doaj.org/toc/2045-2322Abstract The development of crop varieties tolerant to growth temperature fluctuations and improved nutritional value is crucial due to climate change and global population growth. This study investigated the metabolite patterns of developing barley seed as a function of genotype and growth temperature for ideal vegetable protein production and for augmented β-glucan production. Seeds from three barley lines (Bomi, lys3.a and lys5.f) were sampled eight times during grain filling and analysed for metabolites using gas chromatography-mass spectrometry (GC-MS). The lys3.a mutation disrupts a regulator gene, causing an increase in proteins rich in the essential amino acid lysine, while lys5.f carries a mutation in an ADP-glucose transporter gene leading to a significant increase in production of mixed-linkage β-glucan at the expense of α-glucan. Unique metabolic patterns associated with the tricarboxylic acid cycle, shikimate-phenylpropanoid pathway, mevalonate, lipid and carbohydrate metabolism were observed for the barley mutants, whereas growth temperature primarily affected shikimate-phenylpropanoid and lipid metabolism. The study applied recently developed GC-MS metabolomics methods and demonstrated their successful application to link genetic and environmental factors with the seed phenotype of unique and agro-economically important barley models for optimal vegetable protein and dietary fibre production.Bekzod KhakimovMorten Arendt RasmussenRubini Maya KannangaraBirthe Møller JespersenLars MunckSøren Balling EngelsenNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-12 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Bekzod Khakimov Morten Arendt Rasmussen Rubini Maya Kannangara Birthe Møller Jespersen Lars Munck Søren Balling Engelsen From metabolome to phenotype: GC-MS metabolomics of developing mutant barley seeds reveals effects of growth, temperature and genotype
description	Abstract The development of crop varieties tolerant to growth temperature fluctuations and improved nutritional value is crucial due to climate change and global population growth. This study investigated the metabolite patterns of developing barley seed as a function of genotype and growth temperature for ideal vegetable protein production and for augmented β-glucan production. Seeds from three barley lines (Bomi, lys3.a and lys5.f) were sampled eight times during grain filling and analysed for metabolites using gas chromatography-mass spectrometry (GC-MS). The lys3.a mutation disrupts a regulator gene, causing an increase in proteins rich in the essential amino acid lysine, while lys5.f carries a mutation in an ADP-glucose transporter gene leading to a significant increase in production of mixed-linkage β-glucan at the expense of α-glucan. Unique metabolic patterns associated with the tricarboxylic acid cycle, shikimate-phenylpropanoid pathway, mevalonate, lipid and carbohydrate metabolism were observed for the barley mutants, whereas growth temperature primarily affected shikimate-phenylpropanoid and lipid metabolism. The study applied recently developed GC-MS metabolomics methods and demonstrated their successful application to link genetic and environmental factors with the seed phenotype of unique and agro-economically important barley models for optimal vegetable protein and dietary fibre production.
format	article
author	Bekzod Khakimov Morten Arendt Rasmussen Rubini Maya Kannangara Birthe Møller Jespersen Lars Munck Søren Balling Engelsen
author_facet	Bekzod Khakimov Morten Arendt Rasmussen Rubini Maya Kannangara Birthe Møller Jespersen Lars Munck Søren Balling Engelsen
author_sort	Bekzod Khakimov
title	From metabolome to phenotype: GC-MS metabolomics of developing mutant barley seeds reveals effects of growth, temperature and genotype
title_short	From metabolome to phenotype: GC-MS metabolomics of developing mutant barley seeds reveals effects of growth, temperature and genotype
title_full	From metabolome to phenotype: GC-MS metabolomics of developing mutant barley seeds reveals effects of growth, temperature and genotype
title_fullStr	From metabolome to phenotype: GC-MS metabolomics of developing mutant barley seeds reveals effects of growth, temperature and genotype
title_full_unstemmed	From metabolome to phenotype: GC-MS metabolomics of developing mutant barley seeds reveals effects of growth, temperature and genotype
title_sort	from metabolome to phenotype: gc-ms metabolomics of developing mutant barley seeds reveals effects of growth, temperature and genotype
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/7a1c13f3101641938fc35a1308ea83a5
work_keys_str_mv	AT bekzodkhakimov frommetabolometophenotypegcmsmetabolomicsofdevelopingmutantbarleyseedsrevealseffectsofgrowthtemperatureandgenotype AT mortenarendtrasmussen frommetabolometophenotypegcmsmetabolomicsofdevelopingmutantbarleyseedsrevealseffectsofgrowthtemperatureandgenotype AT rubinimayakannangara frommetabolometophenotypegcmsmetabolomicsofdevelopingmutantbarleyseedsrevealseffectsofgrowthtemperatureandgenotype AT birthemøllerjespersen frommetabolometophenotypegcmsmetabolomicsofdevelopingmutantbarleyseedsrevealseffectsofgrowthtemperatureandgenotype AT larsmunck frommetabolometophenotypegcmsmetabolomicsofdevelopingmutantbarleyseedsrevealseffectsofgrowthtemperatureandgenotype AT sørenballingengelsen frommetabolometophenotypegcmsmetabolomicsofdevelopingmutantbarleyseedsrevealseffectsofgrowthtemperatureandgenotype
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From metabolome to phenotype: GC-MS metabolomics of developing mutant barley seeds reveals effects of growth, temperature and genotype

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