Screening of Important Factors in the Early Sepsis Stage Based on the Evaluation of ssGSEA Algorithm and ceRNA Regulatory Network
Background: Sepsis is a dysregulated host response to pathogens. Delay in sepsis diagnosis has become a primary cause of patient death. This study determines some factors to prevent septic shock in its early stage, contributing to the early treatment of sepsis. Methods: The sequencing data (RNA- and...
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oai:doaj.org-article:7a267dcaffd740639bbfae2e317467552021-12-01T02:04:02ZScreening of Important Factors in the Early Sepsis Stage Based on the Evaluation of ssGSEA Algorithm and ceRNA Regulatory Network1176-934310.1177/11769343211058463https://doaj.org/article/7a267dcaffd740639bbfae2e317467552021-11-01T00:00:00Zhttps://doi.org/10.1177/11769343211058463https://doaj.org/toc/1176-9343Background: Sepsis is a dysregulated host response to pathogens. Delay in sepsis diagnosis has become a primary cause of patient death. This study determines some factors to prevent septic shock in its early stage, contributing to the early treatment of sepsis. Methods: The sequencing data (RNA- and miRNA-sequencing) of patients with septic shock were obtained from the NCBI GEO database. After re-annotation, we obtained lncRNAs, miRNA, and mRNA information. Then, we evaluated the immune characteristics of the sample based on the ssGSEA algorithm. We used the WGCNA algorithm to obtain genes significantly related to immunity and screen for important related factors by constructing a ceRNA regulatory network. Result: After re-annotation, we obtained 1708 lncRNAs, 129 miRNAs, and 17 326 mRNAs. Also, through the ssGSEA algorithm, we obtained 5 important immune cells. Finally, we constructed a ceRNA regulation network associated with SS pathways. Conclusion: We identified 5 immune cells with significant changes in the early stage of septic shock. We also constructed a ceRNA network, which will help us explore the pathogenesis of septic shock.Liou HuangChunrong WuDan XuYuhui CuiJianguo TangSAGE PublishingarticleEvolutionQH359-425ENEvolutionary Bioinformatics, Vol 17 (2021) |
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Evolution QH359-425 Liou Huang Chunrong Wu Dan Xu Yuhui Cui Jianguo Tang Screening of Important Factors in the Early Sepsis Stage Based on the Evaluation of ssGSEA Algorithm and ceRNA Regulatory Network |
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Background: Sepsis is a dysregulated host response to pathogens. Delay in sepsis diagnosis has become a primary cause of patient death. This study determines some factors to prevent septic shock in its early stage, contributing to the early treatment of sepsis. Methods: The sequencing data (RNA- and miRNA-sequencing) of patients with septic shock were obtained from the NCBI GEO database. After re-annotation, we obtained lncRNAs, miRNA, and mRNA information. Then, we evaluated the immune characteristics of the sample based on the ssGSEA algorithm. We used the WGCNA algorithm to obtain genes significantly related to immunity and screen for important related factors by constructing a ceRNA regulatory network. Result: After re-annotation, we obtained 1708 lncRNAs, 129 miRNAs, and 17 326 mRNAs. Also, through the ssGSEA algorithm, we obtained 5 important immune cells. Finally, we constructed a ceRNA regulation network associated with SS pathways. Conclusion: We identified 5 immune cells with significant changes in the early stage of septic shock. We also constructed a ceRNA network, which will help us explore the pathogenesis of septic shock. |
format |
article |
author |
Liou Huang Chunrong Wu Dan Xu Yuhui Cui Jianguo Tang |
author_facet |
Liou Huang Chunrong Wu Dan Xu Yuhui Cui Jianguo Tang |
author_sort |
Liou Huang |
title |
Screening of Important Factors in the Early Sepsis Stage Based on the Evaluation of ssGSEA Algorithm and ceRNA Regulatory Network |
title_short |
Screening of Important Factors in the Early Sepsis Stage Based on the Evaluation of ssGSEA Algorithm and ceRNA Regulatory Network |
title_full |
Screening of Important Factors in the Early Sepsis Stage Based on the Evaluation of ssGSEA Algorithm and ceRNA Regulatory Network |
title_fullStr |
Screening of Important Factors in the Early Sepsis Stage Based on the Evaluation of ssGSEA Algorithm and ceRNA Regulatory Network |
title_full_unstemmed |
Screening of Important Factors in the Early Sepsis Stage Based on the Evaluation of ssGSEA Algorithm and ceRNA Regulatory Network |
title_sort |
screening of important factors in the early sepsis stage based on the evaluation of ssgsea algorithm and cerna regulatory network |
publisher |
SAGE Publishing |
publishDate |
2021 |
url |
https://doaj.org/article/7a267dcaffd740639bbfae2e31746755 |
work_keys_str_mv |
AT liouhuang screeningofimportantfactorsintheearlysepsisstagebasedontheevaluationofssgseaalgorithmandcernaregulatorynetwork AT chunrongwu screeningofimportantfactorsintheearlysepsisstagebasedontheevaluationofssgseaalgorithmandcernaregulatorynetwork AT danxu screeningofimportantfactorsintheearlysepsisstagebasedontheevaluationofssgseaalgorithmandcernaregulatorynetwork AT yuhuicui screeningofimportantfactorsintheearlysepsisstagebasedontheevaluationofssgseaalgorithmandcernaregulatorynetwork AT jianguotang screeningofimportantfactorsintheearlysepsisstagebasedontheevaluationofssgseaalgorithmandcernaregulatorynetwork |
_version_ |
1718405910993305600 |